Analysis of genetic diversity among Onobrychis accessions with high agronomic performance by simple sequence repeat (SSR) markers

Sutcu, Tugba; Bilgen, Behiye Banu; Tuna, Metin

doi:10.1007/s11033-022-07584-x

Cited by 3 publications

(6 citation statements)

References 30 publications

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“…Polymorphism information content (PIC) of the loci ranged from 0.124 to 0.244 for 8 SSR loci. It was observed that the results were compatible with each other in the comparison of PIC values using the same SSR markers with our study (Table 3) (Kempf et al, 2016;Ozkan and Bilgen, 2019;Sutcu, 2020;Sutcu et al, 2022). Similar PIC values were calculated in studies conducted by different researchers.…”

Section: Resultssupporting

confidence: 90%

“…The lowest number of allele was obtained from OVK174 whereas the highest number of allele was obtained from the OVK094 marker. When the studies using the same SSR markers were examined, it was observed that the number of alleles was similar to each other (Kempf et al, 2016;Ozkan and Bilgen, 2019;Sutcu, 2020;Sutcu et al, 2022). It can be said that the small differences determined as a result of the comparison are due to the variety in the number and type of Onobrychis species used in these studies (Table 3).…”

Section: Resultsmentioning

confidence: 63%

“…Ozkan and Bilgen (2019) reported the unbiased genetic diversity value as 0.222. Sutcu et al (2022) found the mean uh value as 0.243. When the h value obtained in different studies using various types of markers on sainfoin was examined, similar results were observed.…”

Section: Resultsmentioning

confidence: 98%

“…Shannon's information index (I) was calculated as 0.212 for OVK174 and 0.380 for OVK046 marker. In a previous study conducted with different Onobrychis genotypes by same SSR loci, the mean I value of 0.375 was reported (Sutcu et al, 2022). Ozkan and Bilgen (2019) studied the molecular characterization of two Turkish cultivars and 3 populations of O. viciifolia using same SSRs, and I value was calculated as 0.322.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Yılmaz

Bilgen

Tuna

2023

Tekirdağ Ziraat Fakültesi Dergisi

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Turkey is in a very convenient position for animal husbandry in terms of both natural resources and ecological conditions. Forage crops, which has a very important place in agricultural activities, is the insurance of plant and animal production. Sainfoin is a perennial forage legume species that grown in the northern temperate regions of the world from the Mediterranean region and the Caucasus, and to Central Asia. In this study the genetic diversity of 100 genotypes representing 44 accessions from 18 different Onobrychis species (O. arenaria subsp. arenaria, O. inermis, O. petraea, O. cyri, O. iberica, O. altissima, O. vassilczenkoi, O. conferta subsp. argentea, O. alba subsp. laconica, O. biebersteinii, O. grandis, O. kachetica, O. kemulariae, O. oxyodonta, O. megataphros, O. pallasii, Onobrychis spp., and O. viciifolia) were evaluated using 8 simple sequence repeat (microsatellite) markers. Based on the results, OVK036, OVK094, OVK125, OVM033, OVK161, OVK046, OVM061, and OVK174 loci were polymorphic. The observed number of alleles per SSR locus ranged from 6 to 21 alleles (mean of 11.625). Maximum allele frequency ranged from 0.51 to 0.93 with a mean value of 0.73. The PIC value ranged from 0.124 to 0.244. The mean polymorphism information content of loci was 0.188. Genetic diversity coefficients according to the UPGMA ranged from 0.000 to 0.9375. Cluster analysis divided the 100 sainfoin genotypes into two main groups (Cluster-I and Cluster-II). All diploid genotypes (except for 1 diploid genotype) used in the study formed a separate group within Cluster-I. The results revealed that SSR markers used in this study are useful for molecular characterization and assessing genetic diversity of sainfoin accessions. The obtained SSR alleles and genetic variability in a studied certain loci provided significant information about the genetic structure of sainfoin accessions that could be used as parental lines in sainfoin breeding programs.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Yılmaz

Bilgen

Tuna

2023

Tekirdağ Ziraat Fakültesi Dergisi

Self Cite

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“…In order to increase productivity in forest trees, it is necessary to determine and improve the genotypes that are fast-growing and resistant to biotic and abiotic factors. Genetic diversity is the main resource for establishing genetic breeding programs (Sütcü et al, 2022). Species with high genetic diversity should be determined for breeding studies to reach the desired goal.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Markers: The Efficient Method for the Determination of Pollen Contamination in Conifer Seed Orchards

Bilgen,

Kaya

2023

IJIAAR

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Seed orchards are specialized forest plantations of genetically superior candidate parents selected to produce genetically superior seeds and/or seedlings. Pollen contamination is one of the most important factors affecting the yield, adaptation, and genetic quality of seeds produced from seed orchards in forest tree breeding programs. Potential pollen from forests surrounding the seed orchard is a major concern in tree breeding because it contributes to the loss in genetic gains expected from seed orchard crops.Microsatellite markers are among the most effective markers that are frequently used for creating genetic maps of many species, determining genetic diversity, identifying genetic diseases, population genetic studies, linkage analysis, fingerprint analysis, genotyping, and parental identification. In this study, a bibliometric analysis was performed to quantitatively and qualitatively evaluate the articles published in the last 25 years on seed orchards and pollen contamination. Searching the Web of Science (WOS) with the criteria of "forest trees" and "seed orchards" revealed that 820 articles were published in the last 25 years. It is seen that 77 of these articles are related to pollen contamination. Canada, China, Japan, Sweden, and the USA have been the top contributors to research on pollen contamination in seed orchards of forest trees in the last 25 years, respectively. According to the data obtained, it has been shown that the genetic contamination level of forest tree species in seed orchards is generally between 5% and 90%. It has been determined that microsatellite markers are more widely used in recent years to determine the degree of pollen migration and genetic contamination. It was concluded that studies on pollen contamination were carried out in only two Turkish red pine orchards in Türkiye, which has a total of 189 seed orchards, the majority of which belong to conifers, and that similar studies should be planned in other seed orchards.

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First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques

Yücel,

Kolano,

Cabi

et al. 2024

Journal of Advanced Research in Natural and Applied Sciences

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The origin and genome composition of tetraploid cultivated Onobrychis viciifolia (2n = 4x = 28) were analyzed using dot-blot and genomic in situ hybridization (GISH) techniques. Dot-blot hybridization was used to find a genomic affinity between O. viciifolia and 16 diploid Onobrychis species. The hypothesis on the origin of the O. viciifolia was tested using GISH. Dot-blot analyses suggested a genomic affinity between O. viciifolia and four diploid Onobrychis species (O. kachetica, O. supina, O. pallasii, and O. vaginalis). Hybridization signals were observed on O. viciifolia chromosomes when gDNA of O. kachetica, O. supina, O. pallasi, and O. hypargyrea were used as probes. However, the observed chromosomal distribution of hybridization signals did not resemble GISH results. The observed signals colocalized with 35S rDNA or dispearse signals on all chromosomes were observed depending on the probe. Further investigations using more comprehensive and comparative analysis with both coding and repetitive DNA regions may provide a better understanding of the genome composition and evolution of O. viciifolia.

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Analysis of genetic diversity among Onobrychis accessions with high agronomic performance by simple sequence repeat (SSR) markers

Cited by 3 publications

References 30 publications

Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Microsatellite Markers: The Efficient Method for the Determination of Pollen Contamination in Conifer Seed Orchards

First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques

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