Analysis of genetic diversity among saffron (Crocus sativus) accessions from different regions of Iran as revealed by SRAP markers

Babaei, Saeid; Talebi, Majid; Bahar, Masoud; Zeinali, Hosein

doi:10.1016/j.scienta.2014.03.033

Cited by 38 publications

(21 citation statements)

References 27 publications

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“…The close relationships among saffron accessions revealed in this study can be due to vegetative propagation, artificial selection by humans. These results are in agreement with those obtained by NAMAYANDEH et al, (2013) and BABAEI et al, (2014).…”

Section: Data Scoring and Analysissupporting

confidence: 94%

“…In this study, PIC values ranged from 0.407 to 0.953 with an overall average of 0.824 per primer (Table 2). The average PIC values in this study is higher than those reported in saffron accessions by BABAEI et al, (2014) with SRAP (0.15), EROL et al, (2014) with AFLP (0.34), NEMATI et al, (2012) with SSR (0.34) and NAMAYANDEH et al, (2013) with SSR (0.33). Primers 2298, 2229 and 2393 with 0.953, 0.943 and 0.943 PIC, respectively were identified as the most informative primers.…”

Section: Data Scoring and Analysiscontrasting

confidence: 70%

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Diversity and phylogeny of saffron (Crocus sativus L.) accessions based on iPBS markers

et al. 2018

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This study sought to measure genetic diversity and phylogenetic structure among 196 individuals of saffron from 20 geographically separate accessions from Iran, Spain and Turkey using retrotransposon derived iPBS markers. Twenty-eight primers amplified a total of 179 polymorphic alleles with an average of 6.4 bands per primer. The average of parameters shannon's information index, genetic distance and gene diversity was 0.483, 0.286 and 0.841 respectively. Polymorphic information index ranged from 0.407 to 0.953 with an average of 0.824. Primers 2298, 2229 and 2393 with 0.953, 0.943 and 0.943 PIC respectively, identified as the most informative primers in this study. The results of phylogenetic trees showed that twenty saffron accessions were placed into four major clusters that matched with their geographical locations completely. These results are supported by principal coordinate analysis. Overall, we can confirm that iPBS markers as low cost and high efficient molecular markers are a powerful DNA fingerprinting for assessing genetic diversity and phylogenetic analysis among saffron accessions originating from different geographical regions.

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Section: Data Scoring and Analysissupporting

confidence: 94%

Section: Data Scoring and Analysiscontrasting

confidence: 70%

Diversity and phylogeny of saffron (Crocus sativus L.) accessions based on iPBS markers

et al. 2018

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“…These results may also indicate that iPBS-retrotransposon are more conserved for laurel compared to guava [41], Cicer species [42] and saffron [43]. Additionally, we came to know that iPBS-retrotransposon markers are more informative as compared to other molecular marker systems, like inter-retrotransposon-amplified polymorphism (IRAP) [44], random amplification of polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) [45] and sequence-related amplified polymorphism (SRAP) [46] and can be suggested for any crop to elucidate genetic diversity.…”

Section: Polymorphism In Turkish Laurel Germplasm Revealed By Ipb-retmentioning

confidence: 99%

Exploring the Genetic Diversity and Population Structure of Turkish Laurel Germplasm by the iPBS-Retrotransposon Marker System

Karik¹,

Nadeem

Habyarimana

et al. 2019

Agronomy

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Laurel is a medicinally important plant and is known to the world for its essential oil. Turkey is the main market in the laurel leaf trade by sharing about 90% of the world trade. Here we made an effort to elucidate genetic diversity and population structure of 94 Turkish laurel genotypes collected from 26 provinces and four geographical regions using inter-primer binding site (iPBS) retrotransposon markers. A total of 13 most polymorphic primers were selected which yielded 195 total bands, of which 84.10% were found polymorphic. Mean polymorphism information content (PIC) was (0.361) and diversity indices including mean effective number of alleles (1.36), mean Shannon's information index (0.35) and overall gene diversity (0.22) revealed the existence of sufficient amount of genetic diversity in the studied plant material. Most diversity was found in genotypes collected from the Mediterranean region. Analysis of molecular variance (AMOVA) revealed that most of the variation (85%) in Turkish laurel germplasm is due to differences within populations. Model-based structure, principal coordinate analysis (PCoA) and neighbor-joining algorithms were found in agreement and clustered the studied germplasm according to their collection provinces and regions. This is a very first study exploring the genetic diversity and population structure of laurel germplasm using iPBS-retrotransposon marker system. We believe that information provided in this work will be helpful for the scientific community to take more interest in this forgotten but the medicinally important plant.

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“…The previous studies showed contradictory results RUBIO-MORAGA et al (2009), and ALAVI-KIA et al (2008) could not detect any polymorphism among saffron accessions. The previous results led to classifying saffron as a monomorphic species, while SIK et al (2008), BEIKI et al (2010), NAMAYANDEH et al (2013), BABAEI et al (2014) and EROL et al (2014; reported some diversities among saffron accessions.…”

Section: Resultsmentioning

confidence: 97%

Identification of markers associated with traits for use in marker-assisted selection in saffron

Bayat¹,

Amirnia²,

Özkan³

et al. 2018

Genetika

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Determination of association between molecular markers and agronomic traits provides an excellent tool for marker-assisted selection. In this study, multivariate stepwise regression analysis was used to estimate associations between SSR markers and some agronomic traits in saffron ecotypes. Two-year average values for the measured traits were used for association analyses. The results of stepwise regression analysis revealed significant associations between the traits and some of the studied loci. More than one informative marker was detected for most of the traits. Totally 25 informative SSR markers were identified in two years. Markers SCA382, SCA15 and SCD219 were associated with most traits under both years. These markers are considered to be relatively more reliable. Among the SSR primers, special attention should be drawn to primers SCA382, SCA15, and SCD219, which had the highest associated fragments with most traits in two years and could be considered for use as candidate markers in markerassisted selection.

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Analysis of genetic diversity among saffron (Crocus sativus) accessions from different regions of Iran as revealed by SRAP markers

Cited by 38 publications

References 27 publications

Diversity and phylogeny of saffron (Crocus sativus L.) accessions based on iPBS markers

Diversity and phylogeny of saffron (Crocus sativus L.) accessions based on iPBS markers

Exploring the Genetic Diversity and Population Structure of Turkish Laurel Germplasm by the iPBS-Retrotransposon Marker System

Identification of markers associated with traits for use in marker-assisted selection in saffron

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