Analysis of Genetic Diversity and Phylogenetic Relationships in<i>Crocus Genus</i>of Iran Using Inter-Retrotransposon Amplified Polymorphism

Alavikia, Siamak; Mohammadi, Seyed Abolghasem; Aharizad, Saeid; Moghaddam, Mohammad

doi:10.1080/13102818.2008.10817555

Cited by 74 publications

(55 citation statements)

References 30 publications

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“…It was also used in genetic studies of the diversity or phylogenetic relationships of Oryza L. (Branco et al, 2007), Pisum L. (Smykal, 2006), Diospyros Thunb. (Guo et al, 2006), Musa L. (Teo et al, 2002), and Crocus L. (Alavi-Kia et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic relationships among Secale taxa by using ISSR and IRAP markers and the chromosomal distribution of the AAC microsatellite sequence

Achrem¹,

Kalinka²,

Rogalska³

2014

Turk J Bot

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In most cereals, coding sequences account for less than 20% of the genome (Flavell et al., 1977;Barakat et al., 1997); the remaining part is composed mainly of repetitive sequences, among which microsatellites and retrotransposons are of particular importance. Retrotransposons are ubiquitous and abundant components of grass genomes, constituting a major fraction of repetitive sequences (approximately 10%-60% of the genome). In many species there is a positive correlation between the copy number of retrotransposons and genome size (Pearce et al., 1996;Vicient et al., 2001;Schulman et al., 2004). It is thought that even among individuals within one population there are differences in copy numbers of a given retrotransposon. Retrotransposons are excellent tools for detecting genetic diversity as they are major generators of genomic changes. Sequences generated from retrotransposon-based molecular markers are often more polymorphic than sequences generated from random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), or restriction fragment length polymorphism (RFLP). Detection of inter-retrotransposon amplified polymorphisms (IRAPs) became a retrotransposon-based fingerprinting technique. IRAP markers are generated by amplification of sequences, embedded between 2 retrotransposons, using outward-facing primers annealing to long terminal repeat (LTR) target sequences. This marker system was used for the first time in barley, based on the BARE-1 retrotransposon (Kalendar et al., 1999;Vicient et al., 1999). It was also used in genetic studies of the diversity or phylogenetic relationships of Oryza L.

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Section: Introductionmentioning

confidence: 99%

Assessment of genetic relationships among Secale taxa by using ISSR and IRAP markers and the chromosomal distribution of the AAC microsatellite sequence

Achrem¹,

Kalinka²,

Rogalska³

2014

Turk J Bot

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“…There are few articles that used molecular and biochemical approaches in an attempt to classify and clarify the systematic and phylogeny of this genus (Alavi-Kia et al, 2008;Frizzi et al, 2007;Grilli-Caiola et al, 2004;Keify and Beiki, 2012;Rubio-Moraga et al, 2009;Rubio-Moraga et al, 2010;Sik et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…AFLP has been found an effective tool for identifying genetic variability in saffron (Crocus sativus L.) of different origin (Siracusa et al, 2012). Reterotransposons have also been used for studying the genetic diversity among different saffron species, and genetic variation was observed within and between species, whereas in some cases variation was found among ecotypes of the same species from different geographical regions (Alavi-Kia et al, 2008). Use of molecular markers as a tool for identification of variability among different saffron clones is an important area for improvement of this crop through breeding.…”

Section: Genetic Relationshipmentioning

confidence: 99%

“…This limited genetic diversity in saffron is attributed to its asexual propagation, followed by successive selection during breeding efforts (Alavi Kia et al, 2008). Most of the researchers reveal that saffron is monomorphic in nature by using RAPD, SSR and ISSR markers.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have demonstrated that the genotypic diversity of C. sativus is extremely low (Alavi Kia et al, 2008;Rubio-Moraga et al, 2009). This limited genetic diversity in saffron is attributed to its asexual propagation, followed by successive selection during breeding efforts (Alavi Kia et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Saffron-Potential Candidates for Crop Improvement

et al. 2015

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In this study, thirty one (31) morphologically distinct selections of saffron crop were used for molecular characterization. Molecular characterization was done through SSR, ISSR and RAPD markers. RAPD and ISSR markers showed significant variation; however, SSR markers did not reveal any variation between the selected clones. The Jaccard's similarity coefficient ranged from 0.94 to 1.00 with an average of 0.98 among all 31 selections used. Minimum similarity value (0.94) was observed between CITH-S-107 and PAM-S-116 selections. The study provides sufficient knowledge to identify clones with better stigma characteristics for further crop improvement programs.

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Molecular and chemical characterization of mutant and nonmutant genotypes of saffron grown in Saudi Arabia

Sharafeldin

Alam

Elkholy

2018

Food Science & Nutrition

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Saffron (Crocus sativus L.) is an important spice and medicinal plant that is cultivated in Asia, Europe, North Africa, and North America. Its morphological and biochemical parameters, such as the changes in the floral parts (six tepals, three stamens, three stigmata), biomass, and chlorophyll content, are primarily affected by environmental conditions. A polymerase chain reaction–rapid amplified polymorphic DNA (PCR‐RAPD) approach was used to analyze the extent of the polymorphisms between C. sativus genotypes grown in the Saudi climate. In this research study, the DNA fingerprints of the stigmata of C. sativus genotypes [K1 & K2 = C. sativus var. cashmerianus, C1 = C. sativus (nonmutant), T1 = mutant (T0‐2B), T2 = mutant (T1‐2B), T3 = mutant (T4‐2A)] were determined according to the floral parts, and a total of 10 decamer primers were used for PCR‐RAPD analysis. Only three pairs of arbitrary primers showed polymorphisms (33.3%–88.2%) in the total genomic DNA extracted from these genotypes. Jaccard's similarity index (JSI) ranged from 0.88 to 1.0. An unweighted pair group method with arithmetic mean (UPGMA) similarity and dendrogram matrix showed that two genotypes (T1‐2B and T4‐2A) were closely related to each other and to the strain CM‐cashmerianus, while the T0 of C. sativus genotype showed divergence.

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Analysis of Genetic Diversity and Phylogenetic Relationships inCrocus Genusof Iran Using Inter-Retrotransposon Amplified Polymorphism

Cited by 74 publications

References 30 publications

Assessment of genetic relationships among Secale taxa by using ISSR and IRAP markers and the chromosomal distribution of the AAC microsatellite sequence

Assessment of genetic relationships among Secale taxa by using ISSR and IRAP markers and the chromosomal distribution of the AAC microsatellite sequence

Molecular Characterization of Saffron-Potential Candidates for Crop Improvement

Molecular and chemical characterization of mutant and nonmutant genotypes of saffron grown in Saudi Arabia

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