Analysis of genetic diversity in oilseed brassica germplasm through ISSR markers and isozyme profiling

Abstract

Eleven Brassica germplasm were characterized through the application of 12 oligonucleotide Inter Simple Sequence Repeat (ISSR) primers. A total 1248 bands were amplified through polymorphic chain reaction and were visualized by agarose gel electrophoresis. Among the amplified marker bands 71.47% were polymorphic in nature and 352 bands were found to be monomorphic. The polymorphic bands of the amplified DNAs mostly ranged between 110 bp and 3 kb. Genetic distance among the germplasm ranged between 0.0468 and 0.7189. Moreover, three isozyme systems such as esterase, acid phosphatase and peroxidase were analyzed for allozyme variability that detected distinct 93 isozyme loci of which nearly 61.9% were polymorphic. Two dendrograms were constructed based on the ISSR profiling and isozyme data obtained through electrophoresis to find out the relatedness and phylogenetic relationship among the investigating Brassica germplasm. The clusters of the phylogenetic tree revealed 4 distinct groups of Brassica based upon their ISSR banding patterns and isozyme analysis. Nei’s genetic distance analysis provided strong information about the existence of variability among the germplasm of Brassica. All the germplasm was found to be clustered according to their respective species. Brassica carinata (Ethiopian mustard) showed individuality from all the germplasm studied and made a different branch in the phylogenetic tree suggesting its diverse origin. From the clustering pattern and genetic relationship obtained with ISSR markers and isozyme analysis, breeders can successfully identify the diverse germplasm from different cluster and use them in their future breeding program.

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

“…It signifies a particular, distinct genomic DNA part that was noticed in PCR extension with a pair of definite primers. [93][94][95] These markers have great potential in plant identification at inter-and intra-specific species or population level. 93,96,97 Previously, the linkage mapping using SCAR markers has been linked with 18-carbon fatty acids in rapeseed.…”

“… 93–95 These markers have great potential in plant identification at inter- and intra-specific species or population level. 93 , 96 , 97 Previously, the linkage mapping using SCAR markers has been linked with 18-carbon fatty acids in rapeseed. Developed SCAR marker (L1L9) shows about 25% genetic difference for 18-carbon fatty acids in rapeseed.…”

Morphological Formation, Fatty Acid Profile, and Molecular Identification of Some Landraces of Ethiopian Brassica as a Promising Crop to Support Breeding Programs

“…The results were congruent with those in [ 48 ], which identified and characterized eight species of Cuscuta genes based on molecular (ITS) and morphological data and used the analysis of phylogeny to confirm the diversity among Cuscuta species. Information about genetic distance among the accessions is helpful in a breeding program [ 49 , 50 ].…”

Genetic diversity and phylogenetic analyses of Vitex agnus castus L. populations using ISSR-PCR and chloroplast DNA trnL intron and trnL-F sequences

“…In recent years, PCR-based DNA marker systems such as AFLP, RFLP, RAPD, SSR and ISSR have been common for investigating the genetic makeup of populations, genetic characterization, genetic diversity, divergence and phylogenetic studies (Li and Ge 2001;Gajera et al 2010;Chen et al 2017;Hocaoglu-Ozyigit et al 2020). ISSR markers have been proposed as a new source of genetic markers that are inherited in Mendelian fashion and are scored as dominant markers (Paul et al 2020). These markers have the role of analyzing genetic diversity through the classification of varieties (Kiani and Siahchehreh 2018).…”