Analysis of genetic diversity of the heat shock protein 70 gene on the basis of abundant sequence polymorphisms in chicken breeds

Abstract

ABSTRACT. This study was designed to detect the sequence variation of the chicken heat shock protein 70 (HSP70) gene. A total of 102 individuals from 8 native Chinese breeds together with Dwarf White Chicken and Red Junglefowl were used to detect sequence variations. The coding regions of the chicken HSP70 gene from 102 individuals were cloned and sequenced. Thirty-six variations were identified, which included 34 single nucleotide polymorphisms and 2 indel mutations. Fifty-seven haplotypes were observed, of which, 43 were breed-specific and 14 were shared. There were 7 Red Junglefowl-specific haplotypes, while Haidong and Silkie only had 2 specific haplotypes. Eleven and 3 haplotypes were shared between and within species, respectively. The variation in nucleotide diversity (Pi) and average number of nucleotide (2015) differences (K) among species were consistent. The total Pi of HSP70 was 0.0016, and the total K was 4.1998. The Pi value of Red Junglefowl was the highest (0.0018) and K was 4.8000, while the Pi of Silkie was the lowest (0.0010) and K was 2.5000. These results demonstrated that variation in chicken HSP70 was abundant between and within species.

Molecular Characterization of Heat Shock Protein 70-1 Gene of Capra aegagrus blythi

“…Therefore our study describes, for the first time, polymorphism data on Sindh ibex, which is a vulnerable species, on the basis of HSP70.1 gene. HSP70 has been studied before for the genetic analysis of chicken (Gan et al, 2015). The results of our study showed high haplotype diversity in the population of Sindh ibex at Kirthar National Park.…”

Genetic Diversity of the Structure of HSP70 Gene in Kampung Unggul Balitbangtan (KUB), Walik, and Kate Walik Chickens

“…The nucleotide g.69A>G transition reported by Gan et al (2015) and Chen et al (2016) is identical to the position of g.44A>G transition in the 5′ UTR of the HSP70 gene of chicken (Morimoto et al, 1986). It is because the number reported by Gan et al (2015) and Chen et al (2016) is based on translational start and the number reported by Morimoto et al (1986) is based on transcriptional start.…”

“…Research on the genetic effect of nucleotide substitution in g.69A>G, SNP (single nucleotide polymorphism) in the 5′-flanking region and its association with thermotolerance trait show that only one genotype (GG) proved useful as a marker of heat stress resistance in local Chinese chickens (Gan et al, 2015;Chen et al, 2016). The nucleotide g.69A>G transition reported by Gan et al (2015) and Chen et al (2016) is identical to the position of g.44A>G transition in the 5′ UTR of the HSP70 gene of chicken (Morimoto et al, 1986). It is because the number reported by Gan et al (2015) and Chen et al (2016) is based on translational start and the number reported by Morimoto et al (1986) is based on transcriptional start.…”

“…DNA amplification was performed using the polymerase chain reaction (PCR) with a primary pair of forward, 5′-CGATCTGGCTGCAATCTACG-3′ (Gan et al, 2015) and reverse, 5′-CTGGGAGTCGTTGAAGT AAGCG-3′ (Mazzi et al, 2003) primers based on the chicken HSP70 gene (Morimoto et al, 1986). The amplified PCR target product is 787 bp long, consisting of a 210 bp promoter region, a 112 bp 5′ untranslated region (UTR), and a 465 bp coding region ( Figure 1).…”

Research Note: Possible influence of thermal selection on patterns of HSP70 and HSP90 gene polymorphisms in Thai indigenous and local chicken breeds and red junglefowls

“…Both genes in chickens are located on the fifth chromosome. A recent study on the association between HSP70 and HSP90 genes and cell survival under thermal stress in chickens identified a nucleotide polymorphism in the coding regions of both genes ( Gan et al, 2015 ; Galal and Radwan, 2020 ). A large-scale population genetics study of genetic diversity has been performed on Thai indigenous and local chicken breeds and red junglefowls using microsatellites, showing large genetic variations ( Budi et al, 2023 ).…”