Analysis of genetic relationship in mutant silkworm strains of Bombyx mori using inter simple sequence repeat (ISSR) markers

Velu, Dhanikachalam; Ponnuvel, Kangayam M.; Muthulakshmi, Murugiah; Sinha, R. K.; Qadri, S. M. H.

doi:10.1016/s1673-8527(08)60042-9

Cited by 24 publications

(10 citation statements)

References 28 publications

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“…The number of fragments by ISSR primer may vary considerably, depending on the species. Some authors [3,[30][31][32][33], observed a total number of bands per primer lower than 10 (about five) with the primers UBC-807, UBC-810, UBC-812, UBC-813, and UBC-856, which were relatively uninformative in their respective analyses. However, other authors, such as NAN et al [27,[34][35][36][37] reported an abundant generation of bands for these same primers, showing an average of 20 fragments per primer.…”

Section: Resultsmentioning

confidence: 99%

ISSR Primer Selection for Genetic Variability Analyses with Jaborandi (Pilocarpus microphyllus Stapf ex Wardlew., Rutaceae)

Rocha¹,

Vasconcelos²,

Silva³

et al. 2014

Forest Res

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Pilocarpus microphyllus Stapf ex Wardlew. (jaborandi) is native to the North and Northeast regions of Brazil. Intensive exploitation for the extraction of pilocarpine, a valuable alkaloid for the pharmaceutical industry, has killed off or brought about a loss of vigor of naturally occurring plant populations. As a result, the species is officially listed as an endangered species of the Brazilian flora. Genetic diversity is required for the adaptation of populations to environmental changes, and maintaining it is a central objective for biological conservation. ISSRs are dominant markers widely used in genetic diversity studies of endangered species, allowing for the identification of genotypes and cultivars, as well as helping in phylogenetic studies based on DNA fingerprinting. This study presents an ISSR primer selection for genetic structure analyses of natural populations and cultivated collections of P. microphyllus.

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Section: Resultsmentioning

confidence: 99%

ISSR Primer Selection for Genetic Variability Analyses with Jaborandi (Pilocarpus microphyllus Stapf ex Wardlew., Rutaceae)

Rocha¹,

Vasconcelos²,

Silva³

et al. 2014

Forest Res

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“…Estimates of gene diversity in populations were higher in total (Ht) as compared to those within population diversity (Hs). [142] 2 0 a n d 6 I S S R…”

Section: Causes For Loss Of Genetic Diversitymentioning

confidence: 99%

Genetic Divergence, Implication of Diversity, and Conservation of Silkworm,Bombyx mori

Bindroo

Moorthy

2014

International Journal of Biodiversity

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Genetic diversity is critical to success in any crop breeding and it provides information about the quantum of genetic divergence and serves a platform for specific breeding objectives. It is one of the three forms of biodiversity recognized by the World Conservation Union (IUCN) as deserving conservation. Silkworm Bombyx mori, an economically important insect, reported to be domesticated over 5000 years ago by human to meet his requirements. Genetic diversity is a particular concern because greater genetic uniformity in silkworm can increase vulnerability to pests and diseases. Hence, maintenance of genetic diversity is a fundamental component in long-term management strategies for genetic improvement of silkworm which is cultivated by millions of people around the worlds for its lusture silk. In this paper genetic diversity studies carried out in silkworm using divergent methods (quantitative traits and biochemical and molecular markers) and present level of diversity and factors responsible for loss of diversity are discussed.

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“…The genomic DNA was isolated from silk moths using a standard protocol (Nagaraja and Nagaraju, 1995) as this method yields good-quality genomic DNA (Velu et al, 2008), which was used as a template in the PCRs. …”

Section: Pcr and Amplified Product Analysismentioning

confidence: 99%

Variation in the coding and non-coding sequences of the DH-PBAN gene of diapause and non-diapause silkworm races

Saravanakumar

Ponnuvel

Kamble

2008

JTI

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Diapause hormone (DH) and pheromone biosynthesis-activating neuropeptide (PBAN) code for the diapause gene (DH-PBAN) of Bombyx mori Linnaeus. The DH-PBAN gene sequence from the BLAST database was searched against B. mori genomic gene sequences for similarity. Results showed maximum homology with a genomic contig (accession no. D16230). Primers were designed for the intron, exon and promoter regions of the DH-PBAN gene sequence. The corresponding regions were amplified using genomic DNA as template and products of diapause and non-diapause silkworm races were compared. There was no variation in the 425 bp intron and 254 bp exon regions. However, 1.3% nucleotide sequence variation was observed in selected silkworm races. The promoter region showed 4.2% single nucleotide variation in the diapause and nondiapause silkworm races. The promoter region of the diapause gene showed variations in the sequence of the POU (Pit 1, Oct 1 and Unc-86)-binding site of non-diapause and diapause races of B. mori. In phylogenetic analysis, B. mori formed a cluster with other silkworms of the Bombycidae family, whereas the Noctuidae family formed a separate cluster, indicating that the diapause gene is conserved across the insect taxa.

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Analysis of genetic relationship in mutant silkworm strains of Bombyx mori using inter simple sequence repeat (ISSR) markers

Cited by 24 publications

References 28 publications

ISSR Primer Selection for Genetic Variability Analyses with Jaborandi (Pilocarpus microphyllus Stapf ex Wardlew., Rutaceae)

ISSR Primer Selection for Genetic Variability Analyses with Jaborandi (Pilocarpus microphyllus Stapf ex Wardlew., Rutaceae)

Genetic Divergence, Implication of Diversity, and Conservation of Silkworm,Bombyx mori

Variation in the coding and non-coding sequences of the DH-PBAN gene of diapause and non-diapause silkworm races

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