Analysis of genetic variation of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Central China

Liu, Can; Yi-bao, Ning; Xu, Bin; Gong, Wenzhi; Zhang, Dongdong

doi:10.1292/jvms.15-0570

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…North American genotype (genotype 2) PRRSV was first reported in China in 1995 [ 16 ]. After that, PRRS has become endemic in pig herds nationwide [ 17 , 18 ]. These PRRSV strains were classified as classical PRRSV (C-PRRSV) that clinically characterized by reproductive failure of sows and decreased growth performance and increased mortality in weaning pigs [ 19 , 20 ].…”

Section: General Situation Of Prrsv In Chinamentioning

confidence: 99%

NADC30-Like Porcine Reproductive and Respiratory Syndrome in China

Tian

2017

TOVJ

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NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) has widely spread in China and become locally dominant virus strain in some provinces. Although they are not pathogenic as highly pathogenic PRRSV (HP-RRRSV) that outbreaks since 2006, NADC30-like PRRSVs distinguished themselves by high incidence of recombination with other virus strains which lead to change of virulence. The outbreaks of NADC30-like PRRSV in the vaccinated pig herds suggested that current commercial PRRSV vaccines cannot provide complete protection to the infection. In this review, we have described in detail the current situation of NADC30 PRRSV including epidemiology, genomic characterization, pathogenicity, and efficacy of current commercial vaccines in China.

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Section: General Situation Of Prrsv In Chinamentioning

confidence: 99%

NADC30-Like Porcine Reproductive and Respiratory Syndrome in China

Tian

2017

TOVJ

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“…Representative strains of genotypes 1 and 2 are Lelystad virus and VR-2332, respectively. PRRSV can also be divided into conventional PRRSV (C-PRRSV), special mutant PRRSV (S-PRRSV) and HP-PRRSV strains [ 23 ]. C-PRRSV was first recognized in China in 1996 and the reference strains are CH-1a, HN1, and S1 [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014

Zhang

et al. 2017

J Vet Sci

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Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007–2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%–99.7% and 80.9%–100%, respectively. Phylogenetic analysis based on the NSP2 gene showed that highly pathogenic (HP)-PRRSV was still the dominant virus in southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499–510. Phylogenetic analysis based on the ORF5 gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in southern China from 2007 to 2014, suggesting that, in recent years, the type 2 PRRSV was more diverse in southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution and used to study evolutionary relationships among PRRSV strains in China.

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“…The solid samples were homogenized using QIAGEN TissueLyser II (Qiagen, Germany) followed by total RNA extraction using an OMEGA Total RNA kit I (Omega, USA). Viral cDNA, used as the template in the RT-PCR (Li et al, 2015), was synthesized using MMLV reverse transcriptase (Takara, Japan). A pair of primers targeting the ORF5 gene (forward sequence from 5' to 3': GAG GTGGGCAACYGTTTTAG; reverse sequence from 5' to 3': CAMGMGTAGCGCCAGGACA) was designed and synthesized (BGI, China).…”

mentioning

confidence: 99%

RT-PCR detection of porcine reproductive and respiratory syndrome virus based on the ORF5 gene in mainland China, 2012–2015

Peng¹,

Zhao²,

Liang³

et al. 2017

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Between January 2012 and December 2015, 13,567 clinical samples were collected from healthy pigs and pigs with a history of respiratory symptoms and/or reproductive disorders in 29 provinces of mainland China to detect porcine reproductive and respiratory syndrome virus (PRRSV). From these samples, 7490 were PRRSV-positive (average value, 55.21%). The annual PRRSV detection rate from 2012 to 2015 for each year is 45.67%, 55.99%, 56.91%, and 59.07%, respectively. Phylogenetic analyses revealed that the percentage of highly pathogenic PRRSV strains had a decreasing yearly trend, whereas the vaccine-like strains showed the opposite trend during the years 2012-2015. These data indicate that while the vaccine contributes to PRRSV prevention in China, it might also lead to serious problems. Specifically, we identified 12 NADC30-like PRRSVs during the years 2014-2015, suggesting potential transmission of the newly emerged PRRSV strains in China. Our data contribute to new information about the epidemiology of PRRSV.

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Analysis of genetic variation of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Central China

Cited by 8 publications

References 29 publications

NADC30-Like Porcine Reproductive and Respiratory Syndrome in China

NADC30-Like Porcine Reproductive and Respiratory Syndrome in China

Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014

RT-PCR detection of porcine reproductive and respiratory syndrome virus based on the ORF5 gene in mainland China, 2012–2015

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