Analysis of glucose and xylose metabolism in new indigenous Meyerozyma caribbica strains isolated from corn residues

Tadioto, Viviani; Milani, Letícia; Barrilli, Évelyn Taize; Baptista, Cristina W.; Bohn, Letícia Renata; Dresch, Aline Perin; Harakava, Ricardo; Fogolari, Odinei; Mibielli, Guilherme Martinez; Bender, João Paulo; Treichel, Helen; Stambuk, Boris U.; Müller, Caroline; Alves, Sérgio L.

doi:10.1007/s11274-021-03221-0

Cited by 16 publications

(10 citation statements)

References 48 publications

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“…The concept of biorefineries has attracted attention mainly because some of their products may have a higher added value than second‐generation ethanol 46 . The data presented here show that A. cerevisiae and L. brevis , non‐GMOs isolated from food waste, are able to convert xylose efficiently into organic acids, with a high yield of acetic and lactic acids, even using EG liquor from the APT as substrate.…”

Section: Resultsmentioning

confidence: 82%

The use of xylose from elephant grass liquor and coproduction of organic acids by wild‐type bacteria isolated from food waste

Menegussi,

Montipó,

Fontana

et al. 2023

Biofuels Bioprod Bioref

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During the normal production of ethanol from pretreated lignocellulosic biomass, only the solid fraction containing C6 sugars is generally used by unmodified yeasts in the fermentation process – the remaining liquid fraction, rich in xylose, is used almost exclusively by genetically modified organisms (GMOs) or for other purposes. As the liquor can be used in the manufacture of a series of chemical inputs, microorganisms that metabolize C5 sugars from different sources were initially isolated with the aim of converting these sugars into acetic acid (AA) and lactic acid (LA). Experiments were carried out with pretreated elephant grass liquor, evaluating the effect of adding commonly referenced nutrients through experimental design. Thus, the present work involves the isolation, screening, and molecular characterization of bacteria, easily found in nature, with the potential to metabolize xylose and produce AA and LA, with yields similar or superior to those shown in studies employing GMOs. Using wild‐type strains, high yields were obtained with Acetobacter cerevisiae (0.96 g LA and 0.71 g AA per g total sugars) and Levilactobacillus brevis (0.77 g LA and 0.61 g AA per g total sugars) in relation to the organic acids of interest, highlighting the total conversion of the sugar blend (xylose, glucose, and arabinose) in 24 h. Ammonium citrate also significantly influenced the final yields, being responsible for the increase in production. The results are useful for biorefinery platforms and may contribute to the full use of sugars released in the lignocellulosic pretreatment of raw materials.

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Section: Resultsmentioning

confidence: 82%

The use of xylose from elephant grass liquor and coproduction of organic acids by wild‐type bacteria isolated from food waste

Menegussi,

Montipó,

Fontana

et al. 2023

Biofuels Bioprod Bioref

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“…Many wild yeasts have the potential to accumulate xylitol from the xylose available to the cells. In fact, since several yeasts display the redox imbalance mentioned above, yeast-based xylitol production may become a trend in the biorefinery context (Tadioto et al, 2022;Albarello et al, 2023;Vargas et al, 2023).…”

Section: Xylitol Productionmentioning

confidence: 99%

“…On the other hand, these residues can fortunately have much more profitable and environmentally sustainable destinations if used in second-generation (2G) biorefineries. In these industrial environments, several agro-industrial wastes and by-products of agricultural production, including orange peels and pomace, can be transformed into a myriad of bioproducts through the metabolism of microorganisms such as yeast (Fenner et al, 2022;Tadioto et al, 2022;Scapini et al, 2023b). In this context, this review presents the state of the art and analyzes the biotechnological potential of orange waste in 2G biorefineries with fermentative processes driven by yeast.…”

Section: Introductionmentioning

confidence: 99%

Transforming orange waste with yeasts: bioprocess prospects

Minussi,

Santos,

Scapini

et al. 2024

Rev. Bras. Ciênc. Ambient.

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It is mandatory to make the circular economy a reality, developing ways of transforming waste into valuable products. In this context, investigating the biotechnological potential of different residues is most welcome. This review analyzes how orange waste can be used as biorefinery feedstock to produce different bioproducts using yeasts as the major biocatalysts. In addition to the current orange market, its pectin-rich biomass is described in detail, aiming to elucidate how yeast cells can convert it into ethanol, xylitol, polyphenols, and organic acids (some of them, volatile compounds). Genetic, metabolic, and evolutionary engineering are also analyzed as biotechnological tools to improve the existing processes. Finally, this review also addresses the potential employment of fruit-dwelling yeasts in biorefining pectin-rich biomasses such as orange wastes. All the data presented herein lead to the conclusion that these residues could already be used for noble purposes.

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“…This use will depend on the microorganisms with capacity to metabolise this pentose. Several yeast species stand out as capable candidates for producing many of those products [ 18 , 19 , 20 , 21 ], such as the yeast Meyerozyma caribbica [ 22 ]. This is the new epithet of Pichia caribbica and the teleomorph of Candida fermentati (Division Ascomycota, class Saccharomycetes, order Saccharomycetales, family Debaryomycetaceae—Index Fungorum).…”

Section: Introductionmentioning

confidence: 99%

“…This yeast has been isolated from soils around the world [ 23 ] and in the phylloplane of sugarcane leaves in different provinces of Thailand [ 24 ]. It was also isolated from rotting corn samples [ 22 ], among other niches worldwide. It was identified in the yeast population of the industrial fermentation processes for fuel-ethanol production in northeast Brazil that use sugarcane juice and/or molasses as substrate [ 25 ], as well as in the fermentation process to produce cachaça spirit from sugarcane juice substrate in stills in the state of Pernambuco, northern Brazil [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar

Freitas

Guimarães

et al. 2023

JoF

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The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.

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Analysis of glucose and xylose metabolism in new indigenous Meyerozyma caribbica strains isolated from corn residues

Cited by 16 publications

References 48 publications

The use of xylose from elephant grass liquor and coproduction of organic acids by wild‐type bacteria isolated from food waste

The use of xylose from elephant grass liquor and coproduction of organic acids by wild‐type bacteria isolated from food waste

Transforming orange waste with yeasts: bioprocess prospects

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

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