Analysis of Glutathione, Glutathione Disulfide, Cysteine, Homocysteine, and Other Biological Thiols by High-Performance Liquid Chromatography Following Derivatization by N-(1-Pyrenyl)maleimide

Winters, Roger A.; Żukowski, Janusz; Erçal, Nuran; Matthews, Richard H.; Spitz, Douglas R.

doi:10.1006/abio.1995.1246

Cited by 202 publications

(91 citation statements)

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“…GSH and oxidized glutathione (GSSG) determinations were performed using the HPLC method developed by Winters et al 17 Malondialdehyde levels. Malondialdehyde (MDA) levels, an index of lipid peroxidation, were determined by HPLC methods according to Draper et al 18 and Templar et al 19 Evaluation of oxidative and antioxidative parameters S Sabuncuoglu et al with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of oxidative and antioxidative parameters in pediatric hematopoietic SCT patients

Sabuncuoğlu

Kuşkonmaz

Çetinkaya

et al. 2011

Bone Marrow Transplant

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Conditioning regimens preceding hematopoietic SCT (HSCT) usually consist of high-dose chemotherapy. Chemotherapy and radiation therapy are associated with increased formation of free radicals and depletion of critical plasma and tissue antioxidants. Oxidative stress and antioxidant depletion have been described during the transplantation period in HSCT patients. In a limited number of studies, it was observed that the conditioning regimen resulted in oxidative stress and antioxidant depletion in HSCT patients. The objective of this study was to look for further evidence of oxidative stress and antioxidant status in pediatric HSCT patients. In this study, blood samples were collected from 21 pediatric allo-HSCT patients before and after conditioning therapy. Erythrocyte and plasma malondialdehyde (MDA) levels, erythrocyte reduced and oxidized glutathione (GSH) levels, erythrocyte antioxidant enzymes activities, plasma a-tocopherol and b-carotene levels were determined. After high-dose chemotherapy, erythrocyte and plasma MDA levels increased. Reduced GSH levels decreased whereas oxidized GSH levels increased first and then decreased significantly compared with the values before the chemotherapy regimen. It was also observed that catalase, superoxide dismutase and GSH-S-transferase activities decreased, but there was no change in GSH peroxidase activity. On the other hand, plasma a-tocopherol levels increased, but b-carotene levels did not change.

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Section: Methodsmentioning

confidence: 99%

Evaluation of oxidative and antioxidative parameters in pediatric hematopoietic SCT patients

Sabuncuoğlu

Kuşkonmaz

Çetinkaya

et al. 2011

Bone Marrow Transplant

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“…GSH Determination: GSH levels were determined using the method developed by Winters et al (1995). Briefly, tissue samples were homogenized in serine-borate buffer (100 mM Tris-HCl, 10 mM boric acid, 5 mM L-serine, 1 mM DETAPAC, pH 7.4) and derivatized with 1.0 mM N-(1-pyrenyl)maleimide (NPM) in acetonitrile.…”

Section: Animal Studiesmentioning

confidence: 99%

Antioxidant Effect of Taurine Against Lead-Induced Oxidative Stress

Gürer

Özgüneş

Saygın

et al. 2001

Arch. Environ. Contam. Toxicol.

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139

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Abstract. Oxidative stress is proposed as a molecular mechanism in lead toxicity, which suggests that antioxidants might play a role in the treatment of lead poisoning. The present study was designed to investigate whether taurine has a beneficial effect both on Chinese hamster ovary (CHO) cells and on Fisher 344 (F344) rats following lead exposure. Therefore, oxidative stress parameters (glutathione, malondialdehyde levels, catalase, and glucose-6-phosphate dehydrogenase [G6PD] activities) of lead-exposed CHO cells and F344 rats were determined following taurine treatment. Taurine was found to be effective in (1) increasing glutathione levels that had been diminished by lead; (2) reducing malondialdehyde levels, an end-product of lipid peroxidation; (3) decreasing catalase and erythrocyte G6PD activity, which had been increased by lead exposure; and (4) improving cell survival of CHO cells. However, taurine had no effect on blood and tissue lead levels when 1.1 g/kg/day taurine was administered to F344 rats for 7 days, following 5 weeks of lead exposure (2,000 ppm lead acetate). As a result, taurine seems to be capable of fortifying cells against lead-induced oxidative attack without decreasing lead levels. Therefore, administration of taurine, accompanied by a chelating agent, might increase its effectiveness in the treatment of lead poisoning.

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“…The lower detection limit by HPLC was found to be 50fmol of glutathione following derivatization by N-(1-pyrenyl) maleimide. Various new and other methods have been described for glutathione estimation [27].…”

Section: Discussionmentioning

confidence: 99%

Estimation of plasma glutathione levels in NIDDM patients with and without cardiovascular disease

Vani

Hemanthkumar

2015

Int Jour of Biomed Res

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Diabetes mellitus is a group of metabolic derangements characterised by hyperglycemia resulting from defective insulin secretion or action or both. Persistent hyperglycemia generates reactive oxygen species causing oxidative damage to cells and various biomolecules. In this study the levels of reduced glutathione antioxidant were estimated in NIDDM patients with cardiovascular disease (n=50) by HPLC method and compared to healthy controls (n=50). The results indicated that plasma glutathione levels are significantly decreased showing that antioxidant defence is lowered in diabetic patients with cardiovascular complications.

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Analysis of Glutathione, Glutathione Disulfide, Cysteine, Homocysteine, and Other Biological Thiols by High-Performance Liquid Chromatography Following Derivatization by N-(1-Pyrenyl)maleimide

Cited by 202 publications

References 0 publications

Evaluation of oxidative and antioxidative parameters in pediatric hematopoietic SCT patients

Evaluation of oxidative and antioxidative parameters in pediatric hematopoietic SCT patients

Antioxidant Effect of Taurine Against Lead-Induced Oxidative Stress

Estimation of plasma glutathione levels in NIDDM patients with and without cardiovascular disease

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