Analysis of gossypol by high performance liquid chromatography

Wang, Mu-Zou

doi:10.1016/0378-8741(87)90112-7

Cited by 15 publications

(6 citation statements)

References 6 publications

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“…The chromatogram generated by UHPLC, which requires a shorter run time and offers enhanced sensitivity [ 73 ], showed very narrow well-defined peaks, allowing quantification and detection limits of 0.5 and 0.2 μg/mL, respectively. When compared to Cai et al [ 41 ] for example, who reported a minimum detection limit of 3 μg/mL for gossypol, the present method demonstrated an improved performance for free gossypol analysis.…”

Section: Discussionmentioning

confidence: 58%

“…Previous studies have reported that the stability of gossypol can be affected by the extraction solvent employed [ 40 , 41 ]. Here, 70% acetone was used successfully for efficient extraction.…”

Section: Discussionmentioning

confidence: 99%

“…Here, 70% acetone was used successfully for efficient extraction. At this concentration, solubility of free gossypol is known to be high [ 41 ], with the compound also remaining stable [ 58 ]. When testing 100% acetone to extract spiked gossypol on CSC ( Fig 1B ), by contrast, the presence of an extra peak on the chromatogram indicated the likely conversion of gossypol to another organic group.…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, the KINETEX ® RP-C18 column gradient elution with methanol:water (TFA 0.1%) resulted in a sharper peak, with high sensitivity for gossypol detection. Although alcoholic solvents are known to increase the instability of gossypol [ 58 ], elution in alcoholic systems using short retention times does not negatively affect accurate gossypol detection [ 40 , 41 ]. Under such conditions, with gradient elution, methanol was successfully employed as mobile phase in this method.…”

Section: Discussionmentioning

confidence: 99%

“…Such care has not been adopted in many previously published extraction methodologies for FG. In addition to the risk of FG degradation, others factors, such as the employment of complex solvent mixtures [ 58 , 59 ] and time-consuming extraction methods [ 41 , 42 , 60 ] limits the uptake of a considerable number of published procedures for FG extraction and detection. The official AOCS method indicated to determine free and total gossypol in cottonseed, cottonseed meats and cake is based on High Performance Liquid Chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

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Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake

et al. 2018

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Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%