Analysis of Herbivore Stress- and Phytohormone-Mediated Urease Expression in Soybean (Glycine max)

Menegassi, Angela; Silva, Roberta da Silva e; Carlini, Célia R.; Mithöfer, Axel; Becker-Ritt, Arlete Beatriz

doi:10.1007/s00344-017-9739-x

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…New activation models suggested that nickel ion bound UreE enhances the GTP-uptake by binding with UreG in the presence of magnesium ion and nickel ion also translocated to the UreG and then an apo-urease complex formed Ni 2+ -(UreDFG) 2. GTP hydrolysis by UreG, specifically catalysed by KHCO 3 /NH 4 CO 3 results in the activation of the urease enzyme (Menegassi et al, 2018). The pathway showing the activation mechanism of urease is shown in Fig.…”

Section: Activation Mechanism and Complex Of Ureasementioning

confidence: 99%

A review on distribution, properties, genetic organization, immobilisation and applications of urease

Kumar

Bhardwaj²,

Yadav³

et al. 2022

JANS

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Urease, a nickel-containing metalloenzyme is getting remarkable attention due to a diverse range of applications for mankind. Urease plays a magnificent role in various field like agriculture, analytical, geological phenomena, beverage industry and is an important diagnostic tool. Urease is mainly present in bacteria, fungi, plants and invertebrates and its manifestation in specific genera may open new vistas for its taxonomic position. Various qualitative and quantitative assays are also reported for the estimation of urease enzyme. Urease based biosensors utilizing green synthesis on nanoparticles are also trending. Recently developed inhibitors against urease were discussed in the review. Inhibitory mechanisms involving the structural similarity of the substrate through modification or derivatization can also help in rational drug design by two possible competitive ways either by mimicking monodentate urea binding or binding as a tetrahedral intermediate. Immobilisation of urease through gel entrapment, using non-covalent and covalent protein tags, cross linkage, covalent bonding, using composite films, Teflon, co-precipitation and coating on nanoparticles is also reported. This review also comprised of various application of urease including enhancement of fertility in the soil, cell to cell organization, protection to predators, treatment of various bladder related diseases and infections, analysis of urea and heavy metal ions, biocementation, pollution control by bioleaching of heavy metals and making beverages urea and ethyl carbamate free. As researchers have a keen interest in urease enzyme at present, most of its aspects were incorporated in the article to make it helpful to the scientific community for further research related to the development of new inhibitors and add on applications of urease for the upliftment of the human as well as environment.

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Section: Activation Mechanism and Complex Of Ureasementioning

confidence: 99%

A review on distribution, properties, genetic organization, immobilisation and applications of urease

Kumar

Bhardwaj²,

Yadav³

et al. 2022

JANS

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“…A soja (Glycine Max) é uma leguminosa importante que contém cerca de 40% de proteína, 34% de carboidratos e 21% de óleo (MENEGASSI et al, 2018) sendo a cultura mais produzida no Brasil que, segundo a CONAB (2023) apresentou uma produção de 154,57 milhões de toneladas na safra 2021/22. Após a colheita dos grãos é imprescindível manter ou minimizar sua qualidade, destacando-se a importância das tecnologias para o armazenamento de grãos na etapa pós-colheita, devido a necessidade de manutenção da qualidade dos grãos durante todo o tempo em que são acondicionados, antes de serem transformadas em diversos subprodutos por meio da industrialização (CARVALHO; RODRIGUES, 2023).…”

Section: Introductionunclassified

Termometria digital no armazenamento de grãos de soja

Ferreira Júnior,

Resende,

Bessa

et al. 2024

Cad. Pedagógico

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Considerando a importância da aeração para o armazenamento seguro dos grãos de soja, objetivou-se identificar a influência de aeração manual e automatizada orientadas por sistema de termometria por termopares e sensores digitais, respectivamente, na qualidade de grãos de soja armazenados em silos metálicos. A aeração nestes silos foi orientada de forma manual no silo contendo termopares e automatizada no silo com sistema de termometria digital. Além dos dados da temperatura da massa de grãos, monitorou-se a qualidade dos grãos de soja durante 30 dias de armazenamento em três alturas do silo nas partes inferior, central e superior avaliando no período inicial, após 15 e 30 dias. O uso de sensores digitais de temperatura e umidade relativa se mostrou eficiente para o controle e o monitoramento da temperatura e para o manejo automatizado da aeração em grãos de soja. Devido a heterogeneidade da massa de grãos não se observou influência direta do manejo de aeração na qualidade dos grãos.

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“…Rechenmacher et al [14] has reported that there are three urease isoforms in soybean: the embryospecific encoded by the Eu1 gene, as well as the ubiquitous urease encoded by Eu4 and Eu5.Pure urease extracted from legumes can produce a kit for clinical diagnosis of urea ammonia in serum, regeneration of the dialysate solution in the wearable kidney machine [15].When urease and urea are present at the same time, it provides N nutrition of high concentration, which is continually beneficial to seed germination, plant development [14] and bioregenerative life support systems [16]. Moreover, ureases play a role in defense against insect and phytopathogenic fungi [17] .It was reported that it can increase the hydrolysis rate of urea up to 10 14 times [15]. However, in the field of agronomy, the hydrolysis rate of urea is related to the bioavailability of nitrogen [15].…”

Section: Introductionmentioning

confidence: 99%

Effect of different legume resource and technical parameters on urea hydrolysis

Lang¹,

Li²,

Qiu³

et al. 2022

Global J. Eng. Tech. Adv.

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Urea hydrolysis is widely used in agriculture, environment and other engineering fields, among which urease contained in beans can catalyze urea hydrolysis. The urea hydrolysis activity of legume plant leaching solution (LPLS) was investigated, including soybeans, black beans, mung beans, red beans as well as soybean hulls, soybean leaves, soybean stems and soybean pods. For the high urea hydrolysis activity and economic efficiency, soybean is most suitable for agricultural engineering and other fields than other beans and soybean-related parts extract. The urea hydrolysis activity increases with the concentration of LPLS, while decreases gradually with reaction time. When the heating temperature reaches 25, 35, 45, 55 and 65 ℃, the urea hydrolysis activity is steady and the enzyme activity is high. Enzyme activity decreases after 65 °C (i.e.75, 90 °C). Meanwhile, the soaking time of LPLS has a little effect on the urea hydrolysis activity compared with other factors. These results make a positive contribution to domestic production urease experimental basis.

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Analysis of Herbivore Stress- and Phytohormone-Mediated Urease Expression in Soybean (Glycine max)

Cited by 5 publications

References 36 publications

A review on distribution, properties, genetic organization, immobilisation and applications of urease

A review on distribution, properties, genetic organization, immobilisation and applications of urease

Termometria digital no armazenamento de grãos de soja

Effect of different legume resource and technical parameters on urea hydrolysis

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