Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography

Si-Ahmed, Kahina; Tazerouti, Fairouz; Badjah‐Hadj‐Ahmed, Ahmed Yacine; Aturki, Zeineb; D’Orazio, Giovanni; Rocco, Anna; Fanali, Salvatore

doi:10.1016/j.jpba.2009.08.015

Cited by 41 publications

(29 citation statements)

References 25 publications

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“…Thus, products 5 - 7 were identified as hesperetin 3'- O -α-maltoside ( 5 ), hesperetin 5- O -α-maltoside ( 6 ), and hesperetin 7- O -α-maltoside ( 7 ), respectively. The substrate hesperetin used for this experiment was a chiral compound, the stereochemistry of which was 2 S [12,13,14,15,16,17,18,19] (Sigma-Aldrich Co.). On the other hand, it has been reported that hesperidin epimerizes in C2 to form the 2 S and 2 R epimers [20].…”

Section: Resultsmentioning

confidence: 99%

Production of Hesperetin Glycosides by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activities

Shimoda

Hamada

2010

Nutrients

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The production of hesperetin glycosides was investigated using glycosylation with Xanthomonas campestris and cyclodextrin glucanotransferase (CGTase). X. campestris glucosylated hesperetin to its 3'-, 5-, and 7-O-glucosides, and CGTase converted hesperetin glucosides into the corresponding maltosides. The resulting 7-O-glucoside and 7-O-maltoside of hesperetin showed inhibitory effects on IgE antibody production and on O2- generation from rat neutrophils.

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Section: Resultsmentioning

confidence: 99%

Production of Hesperetin Glycosides by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activities

Shimoda

Hamada

2010

Nutrients

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“…1 Citrus fruits and juices are among the most important nutrient-dense foods. 3 In recent years, the grapefruit (Citrus paradise Mact. 2 The well-established benets of citrus products are mainly due to the presence of most important class of secondary plant metabolites, namely avonoids.…”

Section: Introductionmentioning

confidence: 99%

A MEKC method for naringenin from natural and biological samples

et al. 2015

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The work reported describes the development of a micellar electrokinetic chromatographic (MEKC) method for the determination of naringenin in real samples including grapefruit juice and human blood serum using a PDA detector. The effects of different CE parameters such as concentration and pH of the running buffer, voltage, injection time and concentration of sodium dodecyl sulfate (SDS) were optimized. Under the optimized conditions, naringenin could be well determined within 6 min using 40 mM borate buffer, 40 mM SDS at pH 9.0, at an applied voltage of 25 kV. For the quantitative determination of naringenin (flavonoid aglycone) in grapefruit juice, the naringin (flavonoid glycoside)was hydrolysed and the resulting aglycone was identified and quantified. The calibration curve was linear in the studied concentration range of 0.1 to 50 mg mL À1 (R 2 ¼ 0.995). The detection limit and the limit of quantification were found to be 0.05 and 0.19 mg mL À1 , respectively.

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“…The great advantage of this kind of methodology is the low flow rate and corresponding low solvent consumption making this technology environmentally friendly. Nano‐LC has also been successfully applied to the analysis of hesperetin (H) enantiomers in biological fluids .…”

Section: Introductionmentioning

confidence: 99%

A nano‐LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential

et al. 2014

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In this study, a simple and rapid methodology to analyze and quantify principal flavanones in citrus fruit juices through the use of a nano-LC/UV-Vis apparatus, employing a 75 μm id capillary column packed with sub-2 μm particles C18 stationary phase for 10 cm, was developed. All compounds were baseline resolved working with a step gradient elution mode in 10 min. The developed analytical method was validated and the resulting RSD% for intra- and interday repeatability, related to retention time and peak area, were <4.7 and 5.5%, respectively. LOD and LOQ values corresponded to 0.40 and 1.56 μg/mL for didymin, hesperitin, and narinegenin, while for the other flavanones were 0.78 and 3 μg/mL, respectively. Good linearity with acceptable determination coefficients R(2) was obtained in the range between LOQ concentration and 200 μg/mL (500 μg/mL naringin and hesperidin). Good recovery values were also obtained. Then, the method was applied to the analysis of selected hand-squeezed and commercial citrus juices. Further, the nano-LC system was coupled to a mass spectrometer to confirm analyte identification. Antioxidant capacity of selected samples was also evaluated measured by Folin-Ciocalteu assay and Trolox equivalent antioxidant capacity assay. Results were compared to determine total flavanones concentrations.

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Analysis of hesperetin enantiomers in human urine after ingestion of blood orange juice by using nano-liquid chromatography

Cited by 41 publications

References 25 publications

Production of Hesperetin Glycosides by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activities

Production of Hesperetin Glycosides by Xanthomonas campestris and Cyclodextrin Glucanotransferase and Their Anti-allergic Activities

A MEKC method for naringenin from natural and biological samples

A nano‐LC/UV method for the analysis of principal phenolic compounds in commercial citrus juices and evaluation of antioxidant potential

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