The turbine disk-drum is one of the key components of an aero-engine and its assembly is connected with high-strength refined screws. But due to the uncoordinated rotation and deformation, the screws have abnormal wear damage. Through detailed contact stress analysis of screw body and component level using the finite element method, combined with experimental observation, the mechanism of wear damage of screw surface in screws is determined. It mainly includes the following: Firstly, the finite element method is used to calculate the deformation and stress distribution of the connecting screw of the turbine disk-drum assembly. Then, after the overspeed test, the morphology of the screws disassembled from the disk-drum assembly is evaluated. It was found that the wear degree in the circumferential direction and axial direction of the screw was quite different, that is, the screw wear experiment was consistent with the finite element analysis results. Finally, the influence of different rotation states and screw tightening states on screw wear was compared and analyzed. Conclusions obtained in this paper will be helpful to improve the assembly reliability of turbine drum.