Analysis of HLA‐DQB1 gene polymorphisms in asymptomatic HBV carriers and chronic hepatitis B patients in the Chinese Han population

Abstract

Host genetic factors and environment factors including hepatitis B virus (HBV) genotype are widely viewed as common basis of the different outcomes of HBV infection. Human leucocyte antigen (HLA) plays an important role in immunological reaction to HBV infection. The study aimed to explore whether the HLA-DQB1 allele polymorphisms are associated with the outcomes of HBV infection in the Chinese Han population. One hundred and thirty-four asymptomatic HBV carriers and 139 chronic hepatitis B patients were recruited in this case-control study in Beijing, China. Sequence-specific primers-polymerase chain reaction was used to detect 13 alleles of HLA-DQB1 gene. The frequency distributions of alleles in two groups were analysed using sas 9.1.2 software. After adjustment of confounders the frequencies of HLA-DQB1*0503 allele and *0303 allele in chronic hepatitis B group were statistically significant lower than those in asymptomatic HBV carrier group (P = 0.04; P = 0.05), and the frequency of exposure to alcohol consumption in patients with chronic hepatitis B was clearly higher than that in asymptomatic HBV carrier group (P = 0.004). HLA-DQB1*0503 allele and *0303 allele are independently resistant genetic factors to chronic hepatitis B, and alcohol consumption is the risk factor of chronic hepatitis B.

HLA-DQA1 & DQB1 variants associated with hepatitis B virus-related chronic hepatitis, cirrhosis & hepatocellular carcinoma

“…Hence, to overcome this error, the P value was corrected by Bonferroni inequality method21. In various other studies, DQA1*0102, DQA1*03012627 and DQB1*0302, DQB1*0609, DQB1*0503, DQB1*03031228 alleles have been reported to be showing protective effect from developing chronic HBV infection.…”

Association of polymorphisms of human leucocyte antigen‐DQA1 and DQB1 alleles with chronic hepatitis B virus infection, liver cirrhosis and hepatocellular carcinoma in Chinese

“…However, these associations were no longer significant after Bonferroni correction. In other studies, HLA‐DQA1*0301,*0302, *0501 and DQB1*0301, *0303, *0503 had also been reported to have associations with chronic HBV infection (Thio et al ., 1999; Jiang et al ., 2003; Lu et al ., 2006; Xi‐Lin et al , 2006). However, in our study, we did not ascertain these associations.…”

Hepatitis B virus genotypes, expression quantitative trait loci for ZNRD1-AS1 and their interactions in hepatocellular carcinoma

“…Several studies have demonstrated that the up-regulation of ZNRD1 may inhibit DNA damage and enhance DNA repair capacity, which has been identified as a novel negative modifier in carcinogenesis [11, 12]. As we all kown, genes in the HLA region on chromosome 6 (such as HLA-DP and HLA-DQ , which were expressed as surface antigens and determine the immune response to virus infection) were associated with HBV infection and progression [25]. Which is more evidence that ZNRD1 and HLA play alike roles in cancer risk and virus infection.…”