Analysis of Human Bradykinin Receptor Gene and Endothelial Nitric Oxide Synthase Gene Polymorphisms in End-Stage Renal Disease Among Malaysians

Abstract

The aim of this study was to determine the association of the c.894G>T; p.Glu298Asp polymorphism and the variable number tandem repeat (VNTR) polymorphism of the endothelial nitric oxide synthase (eNOS) gene and c.181C>T polymorphism of the bradykinin type 2 receptor gene (B2R) in Malaysian end-stage renal disease (ESRD) subjects.A total of 150 ESRD patients were recruited from the National Kidney Foundation’s (NKF)dialysis centers in Malaysia and compared with 150 normal healthy individuals. Genomic DNA was extracted from buccal cells of all the subjects. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was carried out to amplify the products and the restricted fragments were separated by agarose gel electrophoresis. Statistical analyses were carried out using software where a level of p <0.05 was considered to be statistically significant.The genotypic and allelic frequencies of the B2R gene (c.181C>T, 4b/a) and eNOS gene (c.894G>T) polymorphisms were not statistically significant (p >0.05) when compared to the control subjects. The B2R and eNOS gene polymorphisms may not be considered as genetic susceptibility markers for Malaysian ESRD subjects.

Nitride oxide synthase 3 and klotho gene polymorphisms in the pathogenesis of chronic kidney disease and age-related cognitive impairment: a systematic review and meta-analysis

“…Our final paper selection identified 21 papers 22 – 42 assessing NOS3 G894T gene polymorphisms in age-related cognitive impairment; three papers 22 , 43 , 44 assessing NOS3 T786C gene polymorphisms in age-related cognitive impairment; five papers 45 – 49 assessing KL G395A gene polymorphisms in age-related cognitive impairment; ten papers 50 – 59 evaluating NOS3 4b/4a gene polymorphisms in CKD; seven papers 55 – 57 , 59 – 62 evaluating NOS3 G894T gene polymorphisms in CKD; three papers 50 , 55 , 63 assessing NOS3 T786C gene polymorphisms in CKD; six papers 64 – 69 assessing KL G395A gene polymorphisms in CKD; and three papers 66 – 68 assessing NOS3 C1818T gene polymorphisms in CKD. This number of papers were searched in PubMed, Embase, Cochrane, and Web of Science; and papers were selected in accordance with inclusion and exclusion criteria.…”