Analysis of human immunodeficiency virus type 1 vector cis- and trans-acting elements production by means of Semliki Forest virus

Vecchio, Claudia Del; Calistri, Arianna; Lombardi, Giuseppe; Celegato, Marta; Biasolo, Maria Angela; Palù, Giorgio; Parolin, Cristina

doi:10.1038/gt.2008.159

Cited by 1 publication

(1 citation statement)

References 68 publications

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“…In a more recent study Semliki Forest virus (SFV) was used to produce recombinant pseudotyped HIV-1 virions. In the SFV/HIV hybrid system SFVderived packaging cassettes allowed encapsidation of SFV/HIV-1 vectors bearing both cis and trans HIV elements, and enabled transfected cells to produce transducing-competent chimeric SFV/HIV particles (Del Vecchio et al, 2009).…”

Section: Packaging Expression Cassette(s) (Helper Plasmid)mentioning

confidence: 99%

Use of HIV as a gene transfer vector.

Pluta

Kacprzak

2009

Acta Biochim Pol

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Despite the extensive research efforts over the past 25 years that have focused on HIV, there is still no cure for AIDS. However, tremendous progress in the understanding of the structure and biology of the HIV virus led to the development of safe and potent HIV-based transgene delivery vectors. These genetic vehicles are referred to as lentiviral vectors. They appear to be better suited for particular applications, such as transgene delivery into stem cells, compared to other viral- and non-viral vectors. This is because Lentivirus-based vectors can efficiently infect nondividing and slowly dividing cells. In the present review article, the current state of understanding of HIV-1 is discussed and the main characteristics that had an impact on vector design are outlined. A historical view on the vector concept is presented to facilitate discussion of recent results in vector engineering in a broader context. Subsequently, a state of the art overview concerning vector construction and vector production is given. This review also touches upon the subject of lentiviral vector safety and related topics that can be helpful in addressing this issue are discussed. Finally, examples of Lentivirus-based gene delivery systems and their applications are presented, with emphasis on animal transgenesis and human gene therapy.

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Section: Packaging Expression Cassette(s) (Helper Plasmid)mentioning

confidence: 99%