Analysis of human tear fluid by Raman spectroscopy

Filik, Jacob; Stone, Nick

doi:10.1016/j.aca.2008.04.036

Cited by 92 publications

(109 citation statements)

References 27 publications

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“…26 Such differences have also been observed in infrared absorption and Raman spectroscopic studies of extracted meibum samples. 27,28 While these studies confirm the relation between meibomian gland dysfunction and an altered composition of the lipid tear film, they provide no information on how the compositional changes of meibum lipids correlate with the structure of the gland. To establish such a correlation, chemical maps of intact meibomian glands with sufficient selectivity to discriminate several lipid constituents are desirable.…”

Section: Introductionmentioning

confidence: 85%

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

et al. 2011

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Abstract. The lipid distribution in the mouse meibomian gland was examined with picosecond spectral antiStokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH 2 stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Section: Introductionmentioning

confidence: 85%

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

et al. 2011

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“…Although commonly used, the question of sample homogeneity and measurement reproducibility is of concern. Already highlighted in the literature in the case of serum and tears analysis [58,63,64], the deposition of a drop following by air drying (also referred as drop coating) does not necessarily generate a homogenous film of proteins on the substrate. Therefore, the repeatability and accuracy of such studies remains limited, often leading to statistically non relevant results.…”

Section: Biophotonicsmentioning

confidence: 99%

Improved protocols for vibrational spectroscopic analysis of body fluids

Bonnier¹,

Petitjean

Baker

et al. 2013

Journal of Biophotonics

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The applications of vibrational spectroscopy to the examination of human blood serum are explored. Although FTIR spectra can be recorded in aqueous solutions at (gelatin) concentrations as low as 100 mg/L, the high-wavenumber region remains obscured by water absorption. Using Raman spectroscopy, high quality spectra of gelatine solutions as low as 10 mg/L can be achieved, also covering the high-wavenumber regions. In human serum, spectral profiles are weak and partially obscured by water features. Dried deposits are shown to be physically and chemically inhomogeneous resulting in reduced measurement reproducibility. Concentration of the serum using commercially available centrifugal filter devices results in an improvement in the spectral intensity and quality. Additionally, in Raman spectroscopy, reduced background and significantly enhanced signal collection is achievable by measurement in an inverted geometry. The improved protocols for spectroscopic measurement of human serum are applicable to a range of bodily fluids and should accelerate potential clinical applications

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“…3 The potential of human tears for the diagnosis of ocular diseases has been evaluated in preliminary studies, Travo et al have shown signicant discrimination (p < 0.0001) of healthy people from patients with keratoconus (degenerative disorder affecting the cornea) and also between patients at an early or advanced stage of disease by high throughput (HT)-FTIR and principal component analysis (PCA). [4][5][6] A study on synovial uid by Raman spectroscopy and k-means analysis, has shown the discrimination of patients with osteoarthritis of low or high severity (74% sensitivity and 71% selectivity).…”

Section: Introductionmentioning

confidence: 99%