Analysis of humoral immune response and cytokines in chickens vaccinated with Eimeria brunetti apical membrane antigen-1 (EbAMA1) DNA vaccine

Hoan, Tran Duc; Thao, Doan Thi; Gadahi, Javaid Ali; Song, Xiaoling; Xu, Lixin; Ren, Yan; Li, Xiangrui

doi:10.1016/j.exppara.2014.04.015

Cited by 28 publications

(10 citation statements)

References 72 publications

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“…Similar promising results have been seen in previous studies where chickens were vaccinated with AMA1 proteins from E. maxima (DNA, recombinant protein and Bacille Calmette-Guérin -vectored immunisations) or E. brunetti (DNA immunisation) and vaccine efficacy was measured in a variety of ways including improved body weight gains and reduced gut lesions after high dose challenge, or reduced oocyst shedding after low dose challenge (Blake et al, 2011;Li et al, 2013;Hoan et al, 2014).…”

Section: Discussionsupporting

confidence: 82%

“…The most promising antigens are derived from early endogenous stages of the Eimeria lifecycle (sporozoite and first generation schizont), which correlates with the findings observed in naturally infected chickens, where these stages induce the strongest anti-Eimeria immunity (McDonald et al, 1986(McDonald et al, , 1988. Several of these antigens have critical roles in host-parasite interactions including proteins that traffic to the parasite surface and beyond via the secretory microneme (MIC) organelles such as MIC2 (Sathish et al, 2011), MIC3 (Lai et al, 2011), MIC4 (Witcombe et al, 2004), and apical membrane antigen (AMA)1, which achieves around 45% immunoprotective capacity against homologous challenge with E. maxima (Blake et al, 2011;Li et al, 2013) or Eimeria brunetti (Hoan et al, 2014).…”

Section: Introductionmentioning

confidence: 56%

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Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens

Pastor-Fernández

Kim

Billington

et al. 2018

International Journal for Parasitology

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Recently, the availability of protocols supporting genetic complementation of Eimeria has raised the prospect of generating transgenic parasite lines which can function as vaccine vectors, expressing and delivering heterologous proteins. Complementation with sequences encoding immunoprotective antigens from other Eimeria spp. offers an opportunity to reduce the complexity of species/strains in anticoccidial vaccines. Herein, we characterise and evaluate EtAMA1 and EtAMA2, two members of the apical membrane antigen (AMA) family of parasite surface proteins from Eimeria tenella. Both proteins are stage-regulated, and the sporozoite-specific EtAMA1 is effective at inducing partial protection against homologous challenge with E. tenella when used as a recombinant protein vaccine, whereas the merozoite-specific EtAMA2 is not. In order to test the ability of transgenic parasites to confer heterologous protection, E. tenella parasites were complemented with EmAMA1, the sporozoite-specific orthologue of EtAMA1 from E. maxima, coupled with different delivery signals to modify its trafficking and improve antigen exposure to the host immune system. Vaccination of chickens using these transgenic parasites conferred partial protection against E. maxima challenge, with levels of efficacy comparable to those obtained using recombinant protein or DNA vaccines. In the present work we provide evidence for the first known time of the ability of transgenic Eimeria to induce cross protection against different Eimeria spp. Genetically complemented Eimeria provide a powerful tool to streamline the complex multi-valent anticoccidial vaccine formulations that are currently available in the market by generating parasite lines expressing vaccine targets from multiple eimerian species.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 56%

Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens

Pastor-Fernández

Kim

Billington

et al. 2018

International Journal for Parasitology

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“…The role of humoral immunity during coccidiosis was debatable with most evidence pointing to a minor function. However, recent studies have demonstrated that antibodies do play an important role in immunity against coccidiosis [25][26][27]. Our data are in high accordance with the recent results.…”

Section: Discussionsupporting

confidence: 95%

Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge

Huang

Zhang

et al. 2015

Parasitology International

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“…TGF-β has been reported to induce protective immunity and increased TNF-α production [ 49 , 50 ]. Hoan et al [ 51 ] also reported that EbAMA1 could induce significantly higher concentrations of TGF-β1 and IL17 in the vaccinated groups. Likewise, in the current research, birds vaccinated with the rEmSAG protein and pVAX1-EmSAG showed higher concentrations of TGF-β1 than that of control groups.…”

Section: Discussionmentioning

confidence: 98%

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

et al. 2018

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BackgroundCoccidiosis is recognised as a major parasitic disease in chickens. Eimeria maxima is considered as a highly immunoprotective species within the Eimeria spp. family that infects chickens. In the present research, the surface antigen gene of E. maxima (EmSAG) was cloned, and the ability of EmSAG to stimulate protection against E. maxima was evaluated.MethodsProkaryotic and eukaryotic plasmids expressing EmSAG were constructed. The EmSAG transcription and expression in vivo was performed based on the RT-PCR and immunoblot analysis. The expression of EmSAG in sporozoites and merozoites was detected through immunofluorescence analyses. The immune protection was assessed based on challenge experiments. Flow cytometry assays were used to determine the T cell subpopulations. The serum antibody and cytokine levels were evaluated by ELISA.ResultsThe open reading frame (ORF) of EmSAG gene contained 645 bp encoding 214 amino acid residues. The immunoblot and RT-PCR analyses indicated that the EmSAG gene were transcribed and expressed in vivo. The EmSAG proteins were expressed in sporozoite and merozoite stages of E. maxima by the immunofluorescence assay. Challenge experiments showed that both pVAX1-SAG and the recombinant EmSAG (rEmSAG) proteins were successful in alleviating jejunal lesions, decreasing loss of body weight and the oocyst ratio. Additionally, these experiments possessed anticoccidial indices (ACI) of more than 170. Higher percentages of CD4+ and CD8+ T cells were detected in both EmSAG-inoculated birds than those of the negative control groups (P < 0.05). The EmSAG-specific antibody concentrations of both the rEmSAG and pVAX1-EmSAG groups were much higher than those of the negative controls (P < 0.05). Higher concentrations of IL-4, IFN-γ, TGF-β1 and IL-17 were observed more in both the rEmSAG protein and pVAX1-SAG inoculated groups than those of negative controls (P < 0.05).ConclusionsOur findings suggest that EmSAG is capable of eliciting a moderate immune protection and could be used as an effective vaccine candidate against E. maxima.

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Analysis of humoral immune response and cytokines in chickens vaccinated with Eimeria brunetti apical membrane antigen-1 (EbAMA1) DNA vaccine

Cited by 28 publications

References 72 publications

Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens

Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens

Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge

Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

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