Analysis of <i>In vitro</i> Fertilizing Capacity to Evaluate the Freezing Procedures of Boar Semen and to Predict the Subsequent Fertility

Sellés, E.; Gadea, J.; Romar, Raquel; Matás, Carmen; Ruiz, Salvador

doi:10.1046/j.1439-0531.2003.00406.x

Cited by 62 publications

(51 citation statements)

References 35 publications

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“…But there are certain variations in pig fertility explained by the boar and semen parameters. Many researchers have studied the relationship between semen parameters and fertility, with conflicting results (Tardif et al, 1999;Sellés et al, 2003;Popwell and Flowers, 2004;Ruiz-Sánchez et al, 2006;Broekhuijse et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

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-The objective of this study was to evaluate the effects of season, natural photoperiod, and room temperature at the housing facility on boar semen characteristics in Uruguay (34º66'S; 56º29'W). For this purpose, 117 ejaculates, obtained from eight adult males collected through 12 consecutive months, were assessed for sperm viability, DNA integrity, abnormalities (total, primary, and secondary), ejaculate volume, and sperm concentration. Viability, total and primary abnormalities, volume, and sperm concentration were affected by season. Sperm viability, volume, and sperm concentration were affected by natural photoperiod. In general, autumn and the decreasing photoperiod had a negative impact on most of the semen characteristics, except for volume. Housing temperature did not affect semen characteristics. In boars living in temperate climates, semen quality is negatively affected during autumn and is related to photoperiod changes; however, the effects of temperature changes in housingdo not affect these seminal characteristics. In this scenario, seasonal differences in semen quality may have a negative effect on sow fertilization. Consequently, semen quality control especially during autumn is imperative for the best boar selection to be used for insemination purposes. Seasonal differences in semen quality may have a negative effect on sow reproductive performance. This issue will be addressed in a future investigation.

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Section: Introductionmentioning

confidence: 99%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

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“…The straws were frozen by placing in contact with nitrogen vapour about 3 cm above the liquid nitrogen level for 20 min in an styrofoam box; and were plunged into liquid nitrogen (-196ºC) for storage. Two weeks later, thawing was done at 50C for 12 sec [26]. Immediately after thawing, the semen was diluted (1:4) with a Modena™ extender.…”

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confidence: 99%

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

2012

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ABSTRACT. The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolkbased extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (P<0.05) and fructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen. Boar semen cryopreservation greatly facilitates the distribution of valuable and desirable genes because the frozen semen can be transported over a long distance; and a stock of frozen semen enables the use of boar that is dead or ill [3]. However, the use of frozen-thawed (FT) boar semen is limited due to the low fertility outcomes compared to the extended fresh semen [9]. During cryopreservation, both physical and chemical factors including the rapid change in temperature or thermal stress, oxidative stress and osmotic stress lead to the damage of the sperm plasma membrane [19]. In general, the egg yolk-based freezing extender of boar semen consists of cryoprotectant, sugar, buffer and antibiotic [16]. Glycerol is the most common cryoprotectant used in the egg yolk-based semen extender for cryopreservation of the boar semen [14] and egg yolk is regarded as a non-permeable cryoprotectant. Sugar is mainly an energy source of the boar sperm. Furthermore, it protects the boar sperm from dehydration and intracellular ice formation during the semen freezing process [27]. Different types of sugar, e.g., trehalose, lactose, fructose, have been used in the freezing extender of boar semen [15,18].Recently, special interest on researches concerning with the energy utilization of the boar sperm have been noticed [13,15,18,20]. In general, the boar sperm is able to utilize a variety of substrates to obtain energy. Generally, monosaccharides are more common energy source used in extended fresh boar semen than disaccharides [20]. However, lactose (disaccharide) is commonly used for the cryopreservation of boar semen by many researchers [8,9,16,17]. In our previous studies [10,17] [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24]. In the boar, information associated with the effect of sugar types used in egg yolk-based semen extender on the FT semen qualities is limited. Therefore, the objective of the present study was to determine the effect of different sugar types (lactose, fructose, glucose and sorbitol) used in egg yolk-based semen extender on the sperm motility, sperm viability, acrosome integrity a...

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“…Then the straws are plunged into liquid nitrogen (-196 ºC) for storage. Thawing is achieved by immersing the straws in water at 50C for 12 sec (Selles et al, 2003). Immediately after thawing, the semen is diluted (1:4) with a Modena™ extender.…”

Section: Semen Freezing and Thawing Proceduresmentioning

confidence: 99%

“…Long term extenders contain complex buffering agent (i.e. HEPES, Tris, TES and MOPs) and antioxidants (i.e., bovine serum albumin (BSA), beta-carotene, cysteine, taurine, vitamin E and ascorbic acid) (Alvarez and Storey, 1995;Gadea, 2003;Funahashi and Sano, 2005), which can maintain semen qualities during cold storage for a longer period than short term extender.…”

Section: Effect Of Long Term Versus Short Term Extenders As Freezing mentioning

confidence: 99%

Cryopreservation of Boar Spermatozoa: An Important Role of Antioxidants

Kaeoket¹

2012

Current Frontiers in Cryopreservation

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Analysis of In vitro Fertilizing Capacity to Evaluate the Freezing Procedures of Boar Semen and to Predict the Subsequent Fertility

Cited by 62 publications

References 35 publications

Seasonal variation in sperm characteristics of boars in southern Uruguay

Seasonal variation in sperm characteristics of boars in southern Uruguay

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

Cryopreservation of Boar Spermatozoa: An Important Role of Antioxidants

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