Analysis of<i>KIT</i>mutations and c-KIT expression in Chinese Uyghur and Han patients with melanoma

Kang, Xiaojing; Shi, Xiuyan; Chen, W.-J.; Pu, Xiongming; Sun, Zhenzhu; Halifu, Yilinuer; Wu, Xiujuan; Yu, Shi-Rong; Liu, W.-X.; Liang, Jianli; Luo, Dingcun; Ren, Danyang

doi:10.1111/ced.12659

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…However, knowledge of melanoma in Asian patients is scant. In Chinese patients, mucosal and acral melanomas account for the majority of melanoma cases among the 4 subtypes [5]. A retrospective analysis showed that the 5-year overall and disease-free survival (DFS) rates and median survival time were 41.6% and 43 months, and 12.3% and 20 months in 522 ethnic Chinese patients respectively, which were substantially worse than those in Caucasian patients [6].…”

Section: Introductionmentioning

confidence: 99%

Methylation-Mediated Silencing of MicroRNA-211 Decreases the Sensitivity of Melanoma Cells to Cisplatin

Liu

Pang

et al. 2019

Med Sci Monit

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Background Malignant melanoma is recalcitrant to most existing chemotherapies, and aberrant expression of miR-211 plays prominent roles in progression of melanoma. However, the trigger mechanism of aberrant miR-211 expression in melanoma is still elusive. Material/Methods We used qRT-PCR to test miR-211 expression. Cell viability assay and mouse xenograft assay were performed to examine the role of miR-211 on the sensitivity of melanoma cells to cisplatin. The epigenetic modification of miR-211 promoter was assess by DNA methylation analysis and DAC treatment. Results In this study, decreased miR-211 expression was detected. Bisulfite sequencing PCR showed that DNA hypermethylation contributed to the downregulation of miR-211 in melanoma tissues. In melanoma cells, overexpressed 211 could enhance the anticancer effect of cisplatin and restoration of miR-211 rendered susceptibility to cisplatin in cisplatin-resistant cells. And the same result was showed in vivo by mouse xenograft assay. What is more, DAC treatment could increase miR-211 expression and EZH2 expression was increased in cisplatin-resistant cells. MiR-211 could be transcriptionally repressed by EZH2 mediated promoter methylation. Conclusions Taken together, our findings revealed that epigenetic modification of miR-211 governed melanoma cell chemosensitivity and were involved in the progression of tumorigenesis.

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Section: Introductionmentioning

confidence: 99%

Methylation-Mediated Silencing of MicroRNA-211 Decreases the Sensitivity of Melanoma Cells to Cisplatin

Liu

Pang

et al. 2019

Med Sci Monit

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“…CSD MM is the most prevalent MM among Chinese Uyghur patients, whereas acral and mucosal MMs are the most prevalent in Chinese Han patients. [ 7 ] CSD (25/60) was found to be the most common type of MM in Uyghur patients in Xinjiang, which was reported by a previous study. [ 7 ] The incidence of BRAF mutations was only 32% (8/25).…”

Section: Discussionmentioning

confidence: 56%

“…[ 7 ] CSD (25/60) was found to be the most common type of MM in Uyghur patients in Xinjiang, which was reported by a previous study. [ 7 ] The incidence of BRAF mutations was only 32% (8/25). The present study was the first to examine the Uyghur patients with MM.…”

Section: Discussionmentioning

confidence: 64%

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Aberrations and clinical significance of BRAF in malignant melanoma

Kang

Zeng²,

Liang³

et al. 2018

Medicine

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Malignant melanoma (MM) is a highly malignant melanocytic tumor, it occurs mostly in the skin, the mucous membrane close to the skin, but also in the tunicae rhagoides and the pia mater. The Uyghur is the largest ethnic group living in the Xinjiang Uyghur Autonomous Region of China, accounting for 46% of the total population of 20 million. Large-scale studies on MMs in Asian countries are limited. This study aimed to investigate BRAF mRNA expression and mutations in Chinese Uyghur patients with MMs and to identify the clinical features associated with these parameters.Formalin-fixed, paraffin wax-embedded tumor sections from 60 MMs were analyzed for BRAF expression using reverse transcription polymerase chain reaction (RT-PCR). Exons 11 and 15 of BRAF were analyzed for the presence of mutations using PCR and DNA sequencing. Sixty MMs were followed by mobile phone for survival analysis.BRAF mRNA expression was higher in MMs than in pigmented moles and normal skin tissues. Fourteen of 60 MMs had BRAF mutations. The frequency of BRAF mutations was significantly higher in patients younger than 60 years (10/28, 4/32, P = .02). A significant difference was observed in the frequency of BRAF mutations among specimens of mucosal, acral, chronic sun-induced damage (CSD), and non-CSD MMs (2/10, 3/19, 8/25, 1/6, P = .002). No significant association was found among BRAF mutations, sex, ulceration, or lymph node metastasis. MMs lymph node metastasis (hazard ratio 2.54 [95% confidence interval 1.062 – 6.066], P = .01) affected survival.This study indicated that BRAF mutations and expression might serve as independent adverse prognostic factors in melanoma.

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“…The melanomas of Uyghur are mainly caused by chronic sunlight injury whereas the Han melanomas are still mainly the mucosal type [101]. [ 6 ] Studying the miRNA profiles in different populations can facilitate understanding the mechanism causing the difference in skin cancer incidence in Xinjiang. In this study, the expression profiles of miRNAs in the skin samples from the Han and Uyghur populations in Xinjiang were investigated.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of microRNAs in the normal skin of the Han and Uyghur populations in Xinjiang Province

Zhao²,

Yuan³

et al. 2018

Medicine

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In this study, we investigated the expression of microRNAs (miRNAs) in the skin samples from the Han and Uyghur populations in Xinjiang, China. The miRNA levels of the normal skin samples from 10 individuals of Uyghur or Han were tested by microarray and the expression differentiations were compared. Among the 3100 probes for microarray, a total of 247 miRNAs were differentially expressed in the Han versus Uyghur population, including 76 upregulated miRNAs and 171 downregulated miRNAs. The most significantly upregulated miRNAs were miR-141-3p, miR-1915-5p, kshv-miR-K12-2-5p, and miR-222-3p. And the most significantly downregulated miRNAs included miR-1207-3p and miR-625-3p. We have confirmed the upregulation of miR-141-3p and miR-1915-5p by qRT-PCR. There were no statistical correlations in the expression of miR-141-3p or miR-1915-5p with the age or gender within each group. Interestingly, the differentially expressed miRNAs were enriched in some cancer-related pathways, such as p53, mitogen-activated protein kinase, and WNT signal pathways. Collectively, these dysregulated expressions of the miRNAs may provide a better understanding of the differences in the incidence and mortality of skin-related carcinoma between the Uyghur and Han populations in Xinjiang.

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Analysis ofKITmutations and c-KIT expression in Chinese Uyghur and Han patients with melanoma

Abstract: In the Xinjiang Uyghur Autonomous Region in China, chronic sun-induced damage MM is the most prevalent MM among Chinese Uyghur patients, whereas acral and mucosal MMs are the most prevalent in Uyghur patients. Mutations in the KIT gene do not correlate with c-KIT expression.

Cited by 10 publications

References 22 publications

Methylation-Mediated Silencing of MicroRNA-211 Decreases the Sensitivity of Melanoma Cells to Cisplatin

Methylation-Mediated Silencing of MicroRNA-211 Decreases the Sensitivity of Melanoma Cells to Cisplatin

Aberrations and clinical significance of BRAF in malignant melanoma

Differential expression of microRNAs in the normal skin of the Han and Uyghur populations in Xinjiang Province

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