Analysis of LncRNA43234-Associated ceRNA Network Reveals Oil Metabolism in Soybean

Abstract

Soybean [Glycine max (Linn.) Merr.] is an important oil crop. Long noncoding RNAs (lncRNAs) play a variety of functions in plants. However, their function in the soybean oil synthesis pathway is yet to be uncovered. Here, the lncRNA43234 gene related to soybean oil synthesis was screened, and the full-length cDNA sequence of the lncRNA was obtained using rapid amplification of cDNA ends. Overexpression of lncRNA43234 increased the content of crude protein in seeds, decreased the content of oleic acid, and affected the content of alanine and arginine in free amino acids. RNA interference of the lncRNA43234 gene decreased the crude protein content in seeds. Quantitative real-time polymerase chain reaction analysis revealed that lncRNA43234 influenced the expression of XM_014775786.1 associated with phosphatidylinositol metabolism by acting as a decoy for miRNA10420, thereby affecting the content of soybean oil. Our results provide insights into how lncRNA-mediated competing endogenous RNA regulatory networks are involved in soybean oil synthesis.

Identification and Genetic Dissection of Resistance to Red Crown Rot Disease in a Diverse Soybean Germplasm Population

“…Every year, about 40% of yield losses are attributed to pathogens and pests [33], of which fungal diseases are known to cause 10-23% of losses [34], requiring much effort in controlling fungal diseases. Soybean crop is a key source of food for humans, making it valuable [35], and it needs to be protected from fungal diseases. Hence, host resistance in controlling soybean RCR is the most economical and sustainable approach.…”

Exploration of GmDof11-lncRNA13082 Module Regulating Oil Synthesis in Plants

“…The crude protein content was assessed using the crude protein assay (GB5009.5�2016). 32 The method for The anthrone colorimetric method was utilized to determine the levels of soluble sugars and starch. The experiment was performed in triplicate, and the t test statistical method was used for statistical analysis of the assays.…”

Update on functional analysis of long non-coding RNAs in common crops

“…The lncRNA43234-miRNA10420-XM_014775781.1 network related to lipid synthesis was screened out by full-length transcriptome sequencing for Wild type (WT) soybean “JN18” (Jishendou 2006) and low linolenic acid mutant “MT72”. Overexpression of lncRNA43234 resulted in increased protein content and decreased oleic acid content in Arabidopsis thaliana seeds ( Ma et al., 2021 ; Zhang A. et al, 2023 ) ( Figure 3C ).…”