Analysis ofSaussureaspecies from tibet using HPLC-DAD-ESI-MSⁿ

Abstract

An HPLC-DAD-ESI-MS n method has been developed for simultaneous quantification of eight major compounds in eight Saussurea species which have long been used as the traditional Tibetan medicines. The method was validated for sensitivity, precision, and accuracy. LODs were from 0.11 to 5.01 μg mL −1 , overall intra-day and inter-day variation was less than 2.70%, and overall recovery was over 98.0%. The correlation coefficients (r 2) of the calibration plots were >0.991. This newly established method was successfully used to reveal difference among the chemical profiles and analyte content of eight Saussurea species collected in Tibet. In addition, by comparison of UV and mass spectra with those of authentic compounds, a total of fifteen peaks were identified. It can be concluded that this is an effective method for quantification and evaluation of the flavonoids and coumarins in the eight species of the genus Saussurea. It can be used as an efficient reference method for development and use of the eight traditional Tibetan medicines by comparing their different characteristics.

Analysis of the Polyphenols of Tobacco Using Pressurized Liquid Extraction (PLE) and Ultra Performance Liquid Chromatography With Electrospray Ionization – Tandem Mass Spectometric Detection (UPLC-ESI-MS/MS)

“…Additionally, it was found that extraction temperatures above 60 °C should be avoided and degassed extraction solvents should be used with pressurized liquid extraction (PLE) due to the chemical reactivity of the phenolics (17,18). The analysis of polyphenols from botanical sources, particularly solanaceous plants like tobacco, using mass spectrometry has been the subject of a significant amount of research and ESI-MS/MS has been used to generate fragmentation data on major and minor polyphenols found in plant extracts (19)(20)(21)(22)(23)(24)(25)(26). Similarities in the fragmentation of the three isomers of chlorogenic acid are well documented (19)(20)(21)(22) and emphasize the need for the optimized chromatographic separation presented here to both distinguish and quantify these compounds in tobacco extracts.…”

Comparative evaluation of chemical profiles of three representative 'snow lotus' herbs by UPLC‐DAD‐QTOF‐MS combined with principal component and hierarchical cluster analyses

“…[36] Peak 7 was identified as 4-hydroxyacetophenone with a main deprotonated ion at m/z 135.0460, in accordance with the reported spectral data. [22] Peak 20 showed base peaks at m/z 455.1988, 819.3888 and 409.1950, in accordance with fragmentations of involucratolactone-β-D-glucoside. [38] Peak 16 showed a base peak at m/z 461.1116, which was assigned as a deprotonated product of homoplantaginin.…”

“…[38] Peak 16 showed a base peak at m/z 461.1116, which was assigned as a deprotonated product of homoplantaginin. [22] Peak 20 showed base peaks at m/z 455.1988, 819.3888 and 409.1950, in accordance with fragmentations of involucratolactone-β-D-glucoside. [39] Peak 21 was identified as another sesquiternene, displaying a quasi-molecular ion m/z 411.2095 and a formiate ion m/z 457.2153.…”

“…[13,21,22] Due to the inconsistent analyzing conditions and the limitation of marker compounds in these studies, a simultaneous, rapid, sensitive, and informative chemical determination of the three herbs is necessary for overall quality evaluation. [13,21,22] Due to the inconsistent analyzing conditions and the limitation of marker compounds in these studies, a simultaneous, rapid, sensitive, and informative chemical determination of the three herbs is necessary for overall quality evaluation.…”

“…In order to determine chemicals from the three herbs, gas chromatography (GC), high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UPLC) were applied as separation methods, coupled with various detectors including diode array detector (DAD) and electrospray ionization mass spectrometry detector (ESI-MS). [13,21,22] Due to the inconsistent analyzing conditions and the limitation of marker compounds in these studies, a simultaneous, rapid, sensitive, and informative chemical determination of the three herbs is necessary for overall quality evaluation. Liquid chromatographytandem mass spectrometry (LC-MS/MS) has been proved to be able to achieve satisfactory resolution of complex samples, with improved separation capacity and accurate mass information generation.…”