Analysis of IFNγ-Induced Migration of Ovarian Cancer Cells

Gaire, Bijaya; Uddin, Mohammad M.; Zou, Yue; Vancurova, Ivana

doi:10.1007/978-1-0716-0247-8_8

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…The scratch area was monitored under a phase-contrast microscope at 0, 24, 48 and 72 h after transfection. The wound width was measured in five random fields using ImageJ software as described [ 32 ] and the average wound width at 72 h was normalized to the average wound width at 0 h.…”

Section: Experimetal Proceduresmentioning

confidence: 99%

Activated heme synthesis regulates glycolysis and oxidative metabolism in breast and ovarian cancer cells

et al. 2021

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Heme is an essential cofactor for enzymes of the electron transport chain (ETC) and ATP synthesis in mitochondrial oxidative phosphorylation (OXPHOS). Heme also binds to and destabilizes Bach1, a transcription regulator that controls expression of several groups of genes important for glycolysis, ETC, and metastasis of cancer cells. Heme synthesis can thus affect pathways through which cells generate energy and precursors for anabolism. In addition, increased heme synthesis may trigger oxidative stress. Since many cancers are characterized by a high glycolytic rate regardless of oxygen availability, targeting glycolysis, ETC, and OXPHOS have emerged as a potential therapeutic strategy. Here, we report that enhancing heme synthesis through exogenous supplementation of heme precursor 5-aminolevulinic acid (ALA) suppresses oxidative metabolism as well as glycolysis and significantly reduces proliferation of both ovarian and breast cancer cells. ALA supplementation also destabilizes Bach1 and inhibits migration of both cell types. Our data indicate that the underlying mechanisms differ in ovarian and breast cancer cells, but involve destabilization of Bach1, AMPK activation, and induction of oxidative stress. In addition, there appears to be an inverse correlation between the activity of oxidative metabolism and ALA sensitivity. Promoting heme synthesis by ALA supplementation may thus represent a promising new anti-cancer strategy, particularly in cancers that are sensitive to altered redox signaling, or in combination with strategies that target the antioxidant systems or metabolic weaknesses of cancer cells.

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Section: Experimetal Proceduresmentioning

confidence: 99%

Activated heme synthesis regulates glycolysis and oxidative metabolism in breast and ovarian cancer cells

et al. 2021

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“…IFN-γ is one of the most important functional cytokines which plays an important role in defense against intracellular pathogens and also cancers (Liu et al, 2020a;Mehta et al, 2020;Zheng et al, 2020a). The results of various studies have shown that the levels of pre-in ammatory cytokines and IFN-γ in patients with solid cancers are naturally elevated (Gaire et al, 2020;Zhang et al, 2020). The study conducted by Qing et al (2020) reported that the levels of IL-1B, IFN-γ, IP-10, and monocyte chemoattractant protein 1 (MCP-1) in patients with COVID-19 was signi cantly high.…”

Section: Discussionmentioning

confidence: 99%

Different Behavioral Patterns of SARS-COV-2 in Patients with Various Types of Cancers: A Role for Chronic Inflammation Induced by Macrophages

Moghanloo

Esboei

Mahmoodzadeh

et al. 2021

Preprint

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SARS-CoV-2 is a single strand RNA virus that causes ongoing pneumonia-like and severe respiratory disease. This disease is very important in immunosuppressed patients. Since not many studies have been done in people with cancer as high risk group, in this study we examined the different behaviors of this virus in different types of cancer. In this study, patients with a variety of solid and hematologic cancers were screened for SARS-CoV-2. The rates of infection, the rate of mortality, in patients with various cancers, as well as in the families of these patients have been studied. In this study, it was found that the prevalence of SARS-CoV-2 in patients with solid cancers was significantly lower than hematological cancers. Also, the mortality rate in hematological cancers was higher than in solid cancers (P<0.05). Assessments the families of these patients, it was found that patients with solid cancers were also less infected than their family members (P<0.05). Researchers of this study attributed these results to the chronic inflammation and M1 macrophages in these patients. It is essential that additional studies are conducted, especially in terms of biological function.

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“…Ovarian cancer SKOV3 and OVCAR3 cells, obtained from the American Type Culture Collection (Rockville, MD, USA), were cultured (5 × 10 5 cells·mL −1 ) in 6‐well plates as described [ 39 , 40 ]. For treatment with IFNγ, human recombinant IFNγ (285‐IF‐100; R&D Systems, Minneapolis, MN) was reconstituted in sterile water.…”

Section: Methodsmentioning

confidence: 99%

IFNγ induces Bcl3 expression by JAK1/STAT1/p65 signaling, resulting in increased IL‐8 expression in ovarian cancer cells

et al. 2023

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We have recently shown that IFNγ, produced during cancer therapy, induces expression of the Bcl3 proto‐oncogene in ovarian cancer (OC) cells, resulting in their increased proliferation, migration, and invasion, but the mechanisms are unknown. Here, we demonstrate that the IFNγ‐induced Bcl3 expression is dependent on JAK1 and STAT1 signaling, and on p65 NFκB. Furthermore, the IFNγ‐induced Bcl3 expression is associated with an increased occupancy of Ser‐727 phosphorylated STAT1 and acetylated histone H3 at the Bcl3 promoter. Our data indicate that Bcl3 promotes expression of the pro‐inflammatory chemokine interleukin‐8 (IL‐8) in OC cells. These findings identify Bcl3 as a novel target of IFNγ/JAK1/STAT1 signaling and suggest that targeting the JAK1/STAT1 pathway may suppress IFNγ‐induced Bcl3 expression in OC.

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Analysis of IFNγ-Induced Migration of Ovarian Cancer Cells

Cited by 6 publications

References 19 publications

Activated heme synthesis regulates glycolysis and oxidative metabolism in breast and ovarian cancer cells

Activated heme synthesis regulates glycolysis and oxidative metabolism in breast and ovarian cancer cells

Different Behavioral Patterns of SARS-COV-2 in Patients with Various Types of Cancers: A Role for Chronic Inflammation Induced by Macrophages

IFNγ induces Bcl3 expression by JAK1/STAT1/p65 signaling, resulting in increased IL‐8 expression in ovarian cancer cells

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