Analysis of in vitro transcription products of intracellular vesicular stomatitis virus RNA polymerase

Abstract

The intracellular transcriptase complex of vesicular stomatitis virus-infected L cells synthesized RNA complementary to the entire infectious virus genome at either 370C or 280C in vitro. Not all sequences were present at the same frequency, however; copies of that segment of the genome common to the LT defective particles were present at 20 to 100 times higher frequently than copies of the genome segment common to the ST defective particle. The less frequent region was transcribed somewhat more effectively at 28°C than at 370C. The results suggest that transcriptional regulation rather than selective degradation is responsible for the differential accumulation of RNA.

In vitro synthesis of a unique RNA species by a T particle of vesicular stomatitis virus

“…This mRNA is capped at the 5' end with 7mG and is polyadenylated at the 3' end (2,7,26). Most of the mRNA is synthesized from the region of the genome coding for the N, NS, M, and G proteins; the region of the genome coding for the L protein does not appear to be efficiently transcribed in vitro (9,16). The in vitro synthesis of RNA by T particles has also been investigated by a number of laboratories (8,13,20,23).…”