Analysis of in vitro Translation of Bean Yellow Mosaic Virus RNA: Inhibition of Proteolytic Processing by Antiserum to the 49K Nuclear Inclusion Protein

Chang, Chih Han; Hiebert, E.; Purcifull, D. E.

doi:10.1099/0022-1317-69-5-1117

Cited by 21 publications

(10 citation statements)

References 17 publications

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“…Many isolates of BYMV are recognized and have been divided into a BYMV subgroup along with the closely related potyviruses on the basis of their overlapping host ranges and common biological and immunological properties (Guyatt et al, 1996; Randles et al, 1980 of linear, positive-sense, single-stranded ribonucleic acid (ssRNA), about 9.6 kb in size, which has a poly (A) tract at the 3'-end (Shukla et al, 1994). Based on the results of some studies (Chang et al, 1988a;1988b), BYMV genome is initially translated into a single polyprotein which is processed by viral-encoded proteases into its mature functional products. This gene expression strategy is defined as a general scheme of potyviral gene expression based on the analyses of other members in the genus Potyvirus (Allison et al, 1986;Dougherty et al, 1989;Shukla et al, 1994;Vance et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

Choi¹,

Yoon²,

Ryu³

et al. 2013

Research in Plant Disease

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Bean yellow mosaic virus (BYMV; genus Potyvirus, family Potyviridae) causes severe losses to various legume species and a number of non-legume species, particularly freesia plants. In a survey of virus diseases in Gyeonggi province, Korea, BYMV isolates were identified from many cultivated freesia species. Here, we determined the complete nucleotide sequences of a BYMV freesia isolate (BYMV-Fr; accession number FJ492961). BYMV-Fr genome consists of 9,545 nucleotides (nt) excluding the poly (A) tail and encodes 3,057 amino acid (aa), with an AUG start and UAG stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of BYMV-Fr was divided to ten proteins and the cleavage sites of each protein were determined. The coat protein (CP) and polyprotein of BYMV-Fr were compared at the aa level with those of the previously reported 4 BYMV isolates. BYMV-Fr shared 90.1 to 97.1 and 91.0 to 92.5% at the CP and polyprotein homology. Interestingly, BYMV-Fr showed identities of a lower level at the nt level of 5' noncoding region (61.4 to 67.6%) and at the aa level of P1 (71.4 to 72.8%), comparing with four BYMV isolates. Based on the aa sequence diversity of CP and polyprotein, phylogenetic analysis with the four BYMV isolates showed two distinct groups and BYMV-Fr and most BYMV isolates were most closely related to the clover yellow vein virus among 52 potyviruses. To our knowledge, this is the first report of the complete genome sequence of BYMV freesia strain.Keywords : Bean yellow mosaic virus, Freesia, Genetic diversity, Potyvirus, RT-PCR, Sequence analysis IntroductionFreesia is a small genus of southern African Iridaceae subfamily Ixioideae, which has been familiar to horticulturists and valued by them for the beauty and fragrance of the flowers. Freesia was first grown in Europe in the mid-18th century and become one of the most popular plants in horticulture in the last half of the 19th century. Appealing shapers of freesias make them suitable line flowering for any arrangement, and their wide range of color increases their versatility (Wang, 2006), resulting that freesias are excellent cut flowers. The flowers are popular used for weddings and make fragrant additions to bouquets and body flowers. Freesias are known to be infected by Bean yellow mosaic virus (BYMV), Freesia mosaic virus, Cucumber mosaic virus, Tobacco rattle virus and ophioviruses (Brunt, 1995;Vaira et al., 2006). BYMV belongs to the genus Potyvirus in the family Potyviridae and the virus is distributed worldwide (Bos, 1970). In comparison with the majority of potyviruses, BYMV has a relatively broad range of plant species. BYMV infects beans, peas, lupins, clovers and dicotyledonous ornamental crops such as gladiolus, bulbous iris, gentians, and some orchids. In particular, BYMV severely affects plant growth, yield and quality of flowers and corms in freesias (Derks et al., 1980;Magie and Poe, 1972). Many isolates of BYMV are recognized and have been divided into a BYMV subgroup along with the closely related ...

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Section: Introductionmentioning

confidence: 99%

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

Choi¹,

Yoon²,

Ryu³

et al. 2013

Research in Plant Disease

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“…Potyviral RNA is translated into a polyprotein containing between 3005 (TVMV) and 3206 (PSbMV) amino acids. This polyprotein is proteolytically processed into at least eight mature proteins by three virusencoded proteinases, the N-terminal (P1) protein (Verchot et al, 1991), the helper component-proteinase (HC-Pro) (Carrington et al, 1989;Carrington & Herndon, 1992), which is also involved in aphid transmissibility, and the nuclear inclusion a protein (NIa-Pro) (Carrington & Dougherty, 1987;Chang et al, 1988;Hellmann et aL, 1988;Garcia et al, 1989;Ghabrial et al, 1990). Other viral proteins with known function are the cytoplasmic inclusion protein (CI) which displays an RNA-dependent ATPase activity characteristic of RNA helicases ; VPg which has been determined to constitute the N-terminal domain of NIa-Pro (Murphy et al, 1990;Dougherty & Parks, 1991), the nuclear inclusion b protein (NIb) thought to be the core replicase (Dougherty & Carrington, 1988) and the capsid protein (CP) (Shukla & Ward, 1989).…”

Section: Introductionmentioning

confidence: 99%

The complete nucleotide sequence of turnip mosaic potyvirus RNA

Nicolas

Laliberté²

1992

Journal of General Virology

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The complete RNA genome of turnip mosaic potyvirus (TuMV) was amplified by seven consecutive reverse transcriptase-polymerase chain reactions and cloned into pUC9. The viral RNA is 9830 nucleotides long and contains a single open reading frame (ORF) of 9489 bases encoding a large polyprotein of 3863 amino acids with a calculated Mr of 358 000. The non-coding region (NCR) preceding the ORF is 129 nucleotides long and has a high AU content (70%). Its predicted secondary structure is characterized by a hairpin loop with a free energy loss of-69.9 kJ/mol. The termination codon is followed by an AU-rich NCR of 209 bases, excluding the poly(A) tail. Seven potential nuclear inclusion a proteinase (NIa-Pro) recognition heptapeptides are found in the polyprotein. Their sequences agree with consensus potyviral NIa-Pro cleavage sequences except for that at the 6K-VPg site, which is characterized by a glutamic acid residue preceding the hydrolysed peptide bond. The TuMV proteins are similar to their corresponding potyviral proteins.

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“…10 kb (Hollings & Brunt, 1981). Several investigations have demonstrated that post-translational proteolytic processing of polyproteins is the mechanism of potyviral genome expression (Dougherty et al, 1985;Allison et aL, 1986;Carrington & Dougherty, 1987;Hellmann et al, 1988 ;Chang et al, 1988). The positions of functional virus proteins in the polyprotein have been proposed for tobacco vein mottling virus (TVMV) (Domier et al, 1986), helper component protein (HC), cylindrical inclusion protein (CI), two nuclear inclusion proteins (NIa and NI0 and the coat protein (CP).…”

Section: Introductionmentioning

confidence: 99%

The Complete Nucleotide Sequence of Plum Pox Virus RNA

Maiß¹,

Timpe²,

Brisske³

et al. 1989

Journal of General Virology

209

143

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SUMMARYThe complete nucleotide sequence of the RNA of an aphid non-transmissible plum pox virus (PPV-NAT) isolate has been determined from five overlapping cDNA clones, cDNA prepared by primer extension was used to determine the 5' terminus. The assembled RNA is 9741 nucleotides in length, excluding a 3' terminal poly(A) sequence. One large open reading frame starts at nucleotide positions 36 to 38 and is terminated with an UAG codon at positions 9522 to 9524. The putative start codon is located at positions 147 to 149. The encoded polyprotein has a predicted Mr of 353"8K. Comparison of cistrons from tobacco vein mottling virus and tobacco etch virus with those predicted for PPV-NAT indicated a similar genome organization. A highly conserved sequence of 12 nucleotides was found in the 5' non-coding region of these three potyviruses. The potential polyadenylation signal from yeast (UAUGU) was found in the 3' non-coding region of PPV-NAT and several other members of the potyvirus group.

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Analysis of in vitro Translation of Bean Yellow Mosaic Virus RNA: Inhibition of Proteolytic Processing by Antiserum to the 49K Nuclear Inclusion Protein

Cited by 21 publications

References 17 publications

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

The complete nucleotide sequence of turnip mosaic potyvirus RNA

The Complete Nucleotide Sequence of Plum Pox Virus RNA

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