Analysis of inflammatory cells and mediators in skin wound biopsies to determine wound age in living subjects in forensic medicine

Fronczek, Judith; Lulf, Ronald; Korkmaz, Halil İbrahim; Witte, Birgit I.; Goot, Franklin R.W. van de; Begieneman, Mark P.V.; Schalkwijk, Casper G.; Krijnen, Paul A.J.; Rozendaal, Lawrence; Niessen, Hans W.M.; Reijnders, Udo

doi:10.1016/j.forsciint.2014.11.014

Cited by 25 publications

(19 citation statements)

References 16 publications

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“…Ethical issues related to the use of tissue from living donors are also important. Indeed, the use of humans in research is subject to approval by the local ethics committee [8,9]. Moreover, as samples from injuries sustained by live human subjects must be obtained in a non-invasive manner (swabbing), so the sample size is frequently inadequate, leading to unreliable, sometimes false-negative results [8,9,15].…”

Section: Common Tissues Used To Date Woundsmentioning

confidence: 99%

“…Yagi et al [61] used immunohistochemistry to evaluate cluster of differentiation (CD)¡14, CD32B, and CD68 expression in human skin wounds, which exhibited greater specificity and reduced the wound age range compared with the assessment using a single marker. Moreover, for accurate wound-age estimation, van de Goot et al [106] and Fronczek et al [8] developed probability scoring systems for the morphological analysis of various indicators. Although these methods yield much information and suggest the utility of wound-age estimation using multiple markers, accurate evaluation of the timing of an injury is hampered by the influence of operator's skill and the many factors involved in injured tissue repair.…”

Section: Data Analysis and Applicationmentioning

confidence: 99%

“…Another obstacle is the availability of, and the not negligible ethical issues involved with, using human specimens with a known time of death [7][8][9]. Therefore, animal studies are essential, but the applicability of the results to humans lacks definitive supporting evidence.…”

Section: Introductionmentioning

confidence: 99%

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Vitality and wound-age estimation in forensic pathology: review and future prospects

Bai

et al. 2018

Forensic Sciences Research

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Determining the age of a wound is challenging in forensic pathology, but it can contribute to the reconstruction of crime scenes and lead to arrest of suspects. Forensic scholars have tended to focus on evaluating wound vitality and determining the time elapsed since the wound was sustained. Recent progress in forensic techniques, particularly high-throughput analyses, has enabled evaluation of materials at the cellular and molecular levels, as well as simultaneous assessment of multiple markers. This paper provides an update on wound-age estimation in forensic pathology, summarizes the recent literature, and considers useful additional information provided by each marker. Finally, the future prospects for estimating wound age in forensic practise are discussed with the hope of providing something useful for further study.

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Section: Common Tissues Used To Date Woundsmentioning

confidence: 99%

Section: Data Analysis and Applicationmentioning

confidence: 99%

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Vitality and wound-age estimation in forensic pathology: review and future prospects

Bai

et al. 2018

Forensic Sciences Research

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“…Fronczek ve ark. yaşayan kişilerden yara bölgesinden alınan yüzeyel deri biopsi materyallerinde inflamatuar hücreleri (MPO, CD45, CD68) ve inflamatuar mediatörleri (MIP-1, IL-8, CML, vitronektin) değerlendirerek yara yaşı değerlen-dirmesinde kullanılabilecek bir skorlama sistemi öner-mişlerdir (37,38). Yagi ve ark (2016) ise transmembran proteini olan ve inflamatuar hücrelerden sentezlenen CD14'ün erken dönem yara iyileşmesinde (1-5) etkin olduğu, en yüksek seviyeyi 12-24 saatte yaptığını saptamıştır (39).Van de Goot ve ark ise (2014) erken dö-nem markerlardan olan fibronektin, CD62p ve faktör 8'i immunhistokimyasal yöntemle belirleyip bir skorlama sistemi oluşturmuştur.…”

Section: Lokal Faktörlerunclassified

Yara Yaşı ve Canlılık Değerlendirmesinde Güncel Yaklaşım

Pakiş

2016

Bull Leg Med

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Adli tıp uygulamalarının ana konularından biri olan yaralar, kişiler canlı iken, ölüm sırasında ve ölüm sonrası gelişebilmektedir. Yaralanmanın kişi canlı iken mi, öldükten sonra mı oluştuğu adli tıbbın önemli sorunlarından biridir. Kesici ya da künt travmalar, yanıklar, boğulmalar gibi travmatik olgularda canlılığın gösterilmesi travma ile ilişki sağlar. Canlılık kanıtlanırsa bu travmanın kişi yaşarken olduğunun kanıtıdır (1,2).Travma ile ölüm arasındaki geçen sürenin belirlenmesi yani yara yaşı tayini de adli tıbbın temel çalışma alanlarındandır. Bu süre dakikalardan aylara hatta yıllara uzayabilmektedir. Çok erken dönemlerde yara yaşı tayini canlılıkla eş değerdir. Travmadan sonraki ilk 30 dakikadaki değişimler hem canlılık bulgusu, hem de yara iyileşmesinin işaretleri olarak kabul edilebilir. En zor soru ÖzetYara yaşı ve canlılık değerlendirilmesi adli tıbbın temel alanlarından biridir. Yaralanmanın kişi canlı iken mi, öldük-ten sonra mı oluştuğu adli tıbbın önemli sorunlarından biridir. Kesici ya da künt travmalar, yanıklar, boğulmalar gibi travmatik olgularda travma ile ilişkiyi sağlar. Canlılık kanıt-lanırsa bu travmanın kişi yaşarken olduğunun kanıtıdır. Literatürde yara yaşı tahmini ve canlılık ile ilgili yapılmış çok sayıda araştırma bulunsa da yapılacak çok sayıda araştırmaya ve araştırmalardan elde edilen bilginin günlük uygulamalara aktarılmasına ihtiyaç vardır. Çalışmada amaç yara yaşı ve canlılık değerlendirmesinde güncel uygulamalar ışığında kullanılan yöntem ve maddeleri adli tıp pratiği açısından değerlendirmektir.Anahtar Kelimeler: Yara yaşı; Canlılık; Adli patoloji. AbstractResearch on vitality and wound age estimation are the classic fields in forensic medicine. Vitality as one of the basic issues in forensic practice deals with the question, whether injuries were caused during lifetime of an individual. It plays a role in connection with traumatic deaths due to sharp and blunt force injuries, burns or drowning. In relation to the vitality of wounds it must be asserted that an injury of the body was caused during lifetime. In spite of large literature data there is still considerable demand of further research and practical transfer of knowledge and techniques to daily casework. The study aims to classify current methods and materials used in evaluation of wound age and viability and determine their practical application in forensic medicine .

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“…Forensic cases present a challenge, as the age assessment of wounds is based on knowledge of healing in other species, and not on healing in the mink per se. Due to the lack of reparative tissue, the early stages of healing are specifically challenging to assess, but offer the unique and timely presence of specific inflammatory cells, which can be studied for more accurate determination of wound age [6, 7]. Wound healing has been studied extensively in a variety of species, especially in laboratory animals used for models of human disease [8–10].…”

Section: Introductionmentioning

confidence: 99%

The effect of color type on early wound healing in farmed mink (Neovison vison)

et al. 2017

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BackgroundIndividual differences of mink, including color type, are speculated to affect the course of wound healing, thereby impacting wound assessment and management on the farms, as well as the assessment of wounds in forensic cases. In this study, we examined the effect of color type on early wound healing in farmed mink. Full thickness excisional wounds (2 × 2 cm) were made on the back in 18 mink of the color types Brown, Silverblue and Blue Iris. Gross and microscopic pathology of the wounds was evaluated 2 days post-wounding together with degree of wound size reduction, presence of bacteria and blood analyses.ResultsPathological examination on day 2 showed the greatest mean wound size reduction in Brown mink (11.0%) followed by Blue Iris (7.9%) and Silverblue (1.6%). Bacteria were cultured from all wounds, and predominantly Staphylococcus species were recovered in mixed or pure culture. Histopathology from day 2 wounds showed a scab overlying necrotic wound edges, which were separated from underlying vital tissue by a demarcation zone rich in polymorphonuclear leukocytes. Fibroblasts and plump endothelial cells were more numerous in the deeper tissues. Complete blood count parameters were within normal ranges in most cases, however, the mink showed mildly to markedly decreased hematocrit and six mink of the color types Silverblue and Blue Iris showed moderately elevated numbers of circulating segmented neutrophils on day 2. There was a marked increase in concentration of serum amyloid A from day 0 to day 2 in all color types.ConclusionsWe have described differences in early wound healing between mink of the color types Brown, Silverblue and Blue Iris by use of an experimental wound model in farmed mink. The most pronounced difference pertained to the degree of wound size reduction which was greatest in Brown mink, followed by Blue Iris and Silverblue, respectively.

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Analysis of inflammatory cells and mediators in skin wound biopsies to determine wound age in living subjects in forensic medicine

Cited by 25 publications

References 16 publications

Vitality and wound-age estimation in forensic pathology: review and future prospects

Vitality and wound-age estimation in forensic pathology: review and future prospects

Yara Yaşı ve Canlılık Değerlendirmesinde Güncel Yaklaşım

The effect of color type on early wound healing in farmed mink (Neovison vison)

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