Analysis of Insect Cuticular Compounds by Non-lethal Solid Phase Micro Extraction with Styrene-Divinylbenzene Copolymers

Ferreira-Caliman, M. J.; Turatti, Izabel Cristina Casanova; Lopes, Norberto Peporine; Zucchi, Ronaldo; Nascimento, Fábio Santos do

doi:10.1007/s10886-012-0109-7

Cited by 12 publications

(11 citation statements)

References 24 publications

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“…We next performed validation of SPME by comparing it to hexane extraction, but we found the two methods resulted in slight quantitative differences ( Fig 2 ). Similarly, previous studies on Drosophila melanogaster [ 25 ], Tenebrio molitor [ 40 ], and Schistocerca gregaria [ 41 ] also revealed this difference in the relative proportions of some CHC components. Unlike quantitative differences, there occurred qualitative differences in CHC profiles of Melipona marginata and Apis mellifera with the two methods, with more or fewer components detected in one but not the other method [ 40 ].…”

Section: Discussionsupporting

confidence: 73%

“…Similarly, previous studies on Drosophila melanogaster [ 25 ], Tenebrio molitor [ 40 ], and Schistocerca gregaria [ 41 ] also revealed this difference in the relative proportions of some CHC components. Unlike quantitative differences, there occurred qualitative differences in CHC profiles of Melipona marginata and Apis mellifera with the two methods, with more or fewer components detected in one but not the other method [ 40 ]. Given that solvent extraction generally involves a whole body wash, rather than a regional investigation in SPME, these differences may be attributed to the non-uniform distribution of cuticular lipids, as recently reported in D .…”

Section: Discussionsupporting

confidence: 73%

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Solid-phase microextraction-based cuticular hydrocarbon profiling for intraspecific delimitation in Acyrthosiphon pisum

et al. 2017

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Insect cuticular hydrocarbons (CHCs) play critical roles in reducing water loss and chemical communication. Species-specific CHC profiles have been used increasingly as an excellent character for species classification. However, considerably less is known about their potential for population delimitation within species. The aims of this study were to develop a solid-phase microextraction (SPME)-based CHC collection method and to investigate whether CHC profiles could serve as potential chemotaxonomic tools for intraspecific delimitation in Acyrthosiphon pisum. Optimization of fibers for SPME sampling revealed that 7 μm polydimethylsiloxane (PDMS) demonstrated the most efficient adsorption of CHCs among five different tested fibers. SPME sampling showed good reproducibility with repeated collections of CHCs from a single aphid. Validation of SPME was performed by comparing CHC profiles with those from conventional hexane extractions. The two methods showed no qualitative differences in CHCs, although SPME appeared to extract relatively fewer short-chained CHCs. While CHC profiles of a given population differed among developmental stages, wing dimorphism types, and host plants, wingless adult aphids showed very low variance in relative proportions of individual CHC components. Reproducibility of CHC profiles was explored further to classify wingless adult morphs of A. pisum from five different geographic regions that showed no variation in mitochondrial COI gene sequences. Our results demonstrate that CHC profiles are useful in intraspecific delimitation in the field of insect chemotaxonomy.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionsupporting

confidence: 73%

Solid-phase microextraction-based cuticular hydrocarbon profiling for intraspecific delimitation in Acyrthosiphon pisum

et al. 2017

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“…The cuticles of honey bees and other Hymenoptera are typically dominated by hydrocarbons 63–65 , which are transferred via the hemolymph from the oenocytes to the insect cuticle 66 , where they function both to prevent desiccation and facilitate communication 63,67–69 . When used for communication, CHC type and quantity can be discriminated with varying degrees of sensitivity for inter- and intra-colonial recognition tasks 70 .…”

Section: Introductionmentioning

confidence: 99%

Stock-specific chemical brood signals are induced by Varroa and Deformed Wing Virus, and elicit hygienic response in the honey bee

Wagoner

Spivak

Hefetz

et al. 2019

Sci Rep

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The health of the honey bee Apis mellifera is challenged by the ectoparasitic mite Varroa destructor , and the numerous harmful pathogens it vectors. Existing pesticide-based Varroa controls are not sustainable. In contrast, one promising approach for improved honey bee health is the breeding of hygienic bees, capable of detecting and removing brood that is parasitized or diseased. In three experiments we find evidence to support the hypothesis that stock-specific chemical brood signals are induced by Varroa and Deformed Wing Virus, and elicit hygienic response in the honey bee. By collecting, analyzing, and running bioassays involving mite-infested and control brood extracts from three honey bee breeding stocks we: 1) found evidence that a transferrable chemical signal for hygienic behavior is present in Varroa -infested brood extracts, 2) identified ten stock-specific hydrocarbons as candidates of hygienic signaling, and 3) found that two of these hydrocarbons linked to Varroa and DWV were also elevated in brood targeted for hygienic behavior. These findings expand our understanding of honey bee chemical communication, and facilitate the development of improved hygienic selection tools to breed honey bees with greater resistance to Varroa and associated pathogens.

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“…(1989) [6]. The total volume of acetone was evaporated up to 3 mL under a constant nitrogen flow for further gas chromatography analysis [7].…”

Section: Methodsmentioning

confidence: 99%

Influence of physicochemical factors on environmental availability and distribution of semiochemicals that affect Varroa destructor and phylogenetically close organisms: classification by VHWOC PCA-clustering

Pérez

Espinosa-Montaño

Moral-Ramírez

et al. 2019

Heliyon

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Varroa destructor parasites Apis mellifera larvae following the interception of the semiochemicals involved in bee communication; thus, the semiochemical availability and distribution pathways take place within different physicochemical environments. The structure of 172 molecules with semiochemical activity on Varroa destructor was used to compute the representative physicochemical descriptors of the thermodynamic partition among different physicochemical environments: vapor pressure (V), Henry's coefficient (H), water solubility constant (W), octanol-water partition coefficient (O) and organic carbon partition coefficient (C); VHWOC. The principal component analysis (PCA) and hierarchical clustering of VHWOC descriptors allowed us to establish the trend in availability and distribution of the semiochemicals resulting in a 4 classes model of physicochemical environments: Class 1, Soluble/Volatile; Class 2, Soluble; Class 3, Contact; Class 4, Adsorbed/Volatile. Our results suggest that semiochemicals can transit between different thermodynamic equilibrium phases depending on environment conditions. The classification prediction of the model was tested on 6 new molecules obtained from ketonic extracts of L5 Apis mellifera drone larvae; locating them in class 4, which was consistent with their molecular structure. This study can be the starting point for the design of synthetic semiochemicals or for the control of Varroa destructor . In addition, the method can be used in the analysis of other semiochemical groups.

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Analysis of Insect Cuticular Compounds by Non-lethal Solid Phase Micro Extraction with Styrene-Divinylbenzene Copolymers

Cited by 12 publications

References 24 publications

Solid-phase microextraction-based cuticular hydrocarbon profiling for intraspecific delimitation in Acyrthosiphon pisum

Solid-phase microextraction-based cuticular hydrocarbon profiling for intraspecific delimitation in Acyrthosiphon pisum

Stock-specific chemical brood signals are induced by Varroa and Deformed Wing Virus, and elicit hygienic response in the honey bee

Influence of physicochemical factors on environmental availability and distribution of semiochemicals that affect Varroa destructor and phylogenetically close organisms: classification by VHWOC PCA-clustering

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