Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

Bukowska, Dorota; Kempisty, Bartosz; Jackowska, M.; Woźna, M.; Antosik, Paweł; Piotrowska, H.; Jaśkowski, J. M.

doi:10.2478/v10181-011-0038-3

Cited by 15 publications

(12 citation statements)

References 28 publications

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“…Recently (Kowalewski et al 2011a), we have speculated that PRL acting through endo-and/or paracrine mechanisms might be involved in endometrial glandular secretory function in the dog. Furthermore, Bukowska et al (2011) reported an increased expression of ITGA2B, ITGB2, and ITGB3 and of VEGF -165, -182, and -188 in the uterus of earlypregnant bitches.…”

Section: Introductionmentioning

confidence: 99%

Expression of genes involved in the embryo–maternal interaction in the early-pregnant canine uterus

et al. 2014

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Although there is no acute luteolytic mechanism in the absence of pregnancy in the bitch, a precise and well-timed embryo-maternal interaction seems to be required for the initiation and maintenance of gestation. As only limited information is available about these processes in dogs, in this study, the uterine expression of possible decidualization markers was investigated during the pre-implantation stage (days 10-12) of pregnancy and in the corresponding nonpregnant controls. In addition, the expression of selected genes associated with blastocyst development and/or implantation was investigated in embryos flushed from the uteri of bitches used for this study (unhatched and hatched blastocysts). There was an upregulated expression of prolactin receptor (PRLR) and IGF2 observed pre-implantation. The expression of PRL and of IGF1 was unaffected, and neither was the expression of progesterone-or estrogen receptor b (ESR2). In contrast, (ESR1) levels were elevated during early pregnancy. Prostaglandin (PG)-system revealed upregulated expression of PGE2-synthase and its receptors, PTGER2 and PTGER4, and of the PG-transporter. Elevated levels of AKR1C3 mRNA, but not the protein itself, were noted. Expression of prostaglandin-endoperoxide synthase 2 (PTGS2) remained unaffected. Most of the transcripts were predominantly localized to the uterine epithelial cells, myometrium and, to a lesser extent, to the uterine stroma. PGES (PTGES) mRNA was abundantly expressed in both groups of embryos and appeared higher in the hatched ones. The expression level of IGF2 mRNA appeared higher than that of IGF1 mRNA in hatched embryos. In unhatched embryos IGF1, IGF2, and PTGS2 mRNA levels were below the detection limit.

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Section: Introductionmentioning

confidence: 99%

Expression of genes involved in the embryo–maternal interaction in the early-pregnant canine uterus

et al. 2014

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“…The only available data indicate an upregulated messenger expression encoding for integrins-α2b, −β2, and −β3, as well as higher uterine activity of matrix metalloproteinases, especially MMP2, in response to free-fl oating embryos (Beceriklisoy et al 2007 ;Bukowska et al 2011 ). The signifi cantly elevated activity of uterine MMPs positively correlates with the activity of both MMP2 and MMP9 in blood serum, which is higher than in nonpregnant animals (Schafer-Somi et al 2005 ).…”

Section: Structural Remodelingmentioning

confidence: 98%

“…Interestingly, this was in contrast to the signifi cantly increased expression of extracellular matrix protein 1 (ECM1) and decreased expression of fi bronectin 1 (FN1) in embryo-exposed uteri(own data, unpublished). Moreover, as a part of induced structural remodeling, increased uterine vascularization was indicated by elevated expression of vascular endothelial growth factors (VEGF) −165, −182, and −188 and one of its receptors, particularly VEGFR-2 (Bukowska et al 2011 ;Schafer-Somi et al 2013 ).…”

Section: Structural Remodelingmentioning

confidence: 99%

“…It needs to be emphasized that all of the aforementioned studies (Schafer-Somi et al 2008Bukowska et al 2011 ;Gram et al 2013 ;Gram et al 2014a , b ;Kautz et al 2014 ) describe changes in the uterine response to free-fl oating canine embryos between days 10 and 12 of gestation. This corresponds to pregnancy stages in other species, i.e., ruminants and pigs, when the embryonic antiluteolytic signals are initiated.…”

Section: Perspectivesmentioning

confidence: 99%

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The Dog: Nonconformist, Not Only in Maternal Recognition Signaling

Kowalewski

Gram

Kautz

et al. 2015

Regulation of Implantation and Establishment of Pregnancy in Mammals

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Although similar at the molecular and cellular levels, endocrine mechanisms governing reproductive function in the domestic dog (Canis familiaris) differ markedly at the regulatory level from those known in other domestic animal species. Some of the events, e.g., the lack of luteolysis in the absence of pregnancy, resulting in similar luteal function and, therefore, hormonal profiles in early pregnant and nonpregnant animals, are species-specific. Consequently, no early gestation marker has so far been identified for the dog. Following implantation, relaxin of fetal placental origin can be detected and used for pregnancy diagnosis. Characterized by the lack of an active luteolytic principle from intra- or extra-luteal sources, the canine reproductive cycle appears to represent a "basic" form of mammalian reproductive function with apparently reduced opportunities for facilitating fecundity and hastening reproduction. Nevertheless, in the dog some kind of mechanism for synchronization between blastocyst development and uterine preparation for pregnancy must have evolved in order to support gestation. Driven by this assumption, studies including our recent investigations have been initiated aimed at characterizing some of the embryo-mediated effects of the preimplantation embryo on the canine uterus. Moreover, the lack of a uterine luteolysin and consequently the absence of a need to develop an antiluteolytic strategy make the dog an interesting model for investigating early evolutionary mechanisms involved in the preparation for implantation and ensuring embryo survival. These mechanisms result in an inverse relationship between the duration of pregnancy and of the nonpregnant cycle in the dog, compared with all other domestic animal species.

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“…Ekspresja badanych genów wzrastała w pierwszej fazie procesu implantacji, co może być związane z indukcją szczególnych mechanizmów odpowiedzialnych za receptywność macicy podczas adhezji zarodków (5). Podobne wyniki uzyskano u królika i świni, zwięk-szoną ekspresję czynnika VEGF-A oraz jego receptora w endometrium odnotowano w okresie implantacyjnym.…”

Section: Czynnik Wzrostu śRódbłonka Naczyniowego (Vascular Endotheliaunclassified

Relevant aspects of the influence of chosen growth factors on the activity of the female reproductive system

Woźna¹,

Rybska²,

Swierczewska³

et al. 2017

Medycyna Weterynaryjna

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*) Projekt został sfinansowany ze środków Narodowego Centrum Nauki przyznanych na podstawie decyzji DEC-2013/09/N/NZ5/0183. Woźna M., Rybska M., Świerczewska M., Jaśkowski J. M.Relevant aspects of the influence of chosen growth factors on the activity of the female reproductive system Summary The endometrium of the uterus is a highly dynamic structure in terms of its changes during the various stages of the sexual cycle. These changes are the result of cyclical fluctuations in the concentrations of steroid hormones and local factors of an auto -and paracrine -nature. This condition indicates that the causes of degenerative processes of the uterus must be sought not only in disorders of the hormonal profile and bacterial infections but also disorders at the molecular level. Factors that may play a key role in the formation and development of various pathologies of the female reproductive system include growth factors and their receptors (growth factors -GFs). Discussing these growth factors in the work may provide useful molecular markers that identify pathological conditions of the endometrium. Subject to expression in the endometrium, they are involved in the regulation of cell proliferation, migration and secretion of the glandular epithelium, they also regulate physiological and pathological angiogenesis, revealing strong pro-inflammatory effects. In this research, the authors present an overview of current scientific reports indicating that changes in the expression of studied factors, and thus disturbances in their effects, may constitute one of the causes of pathogenesis within the uterus in many animal species.

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Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

Cited by 15 publications

References 28 publications

Expression of genes involved in the embryo–maternal interaction in the early-pregnant canine uterus

Expression of genes involved in the embryo–maternal interaction in the early-pregnant canine uterus

The Dog: Nonconformist, Not Only in Maternal Recognition Signaling

Relevant aspects of the influence of chosen growth factors on the activity of the female reproductive system

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