Analysis of Interaction Between Interfacial Structure and Fibrinogen at Blood-Compatible Polymer/Water Interface

Ueda, Tomoya; Murakami, Daiki; Tanaka, Masaru

doi:10.3389/fchem.2018.00542

Cited by 27 publications

(25 citation statements)

References 38 publications

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“…Research supporting this result was reported recently from the interaction force measurement between fibrinogen and PMEA, 89 and the molecular weight dependence of PMEA on the interfacial structure and the blood compatibility. 90 We speculate that the water-rich region prevents the adsorption of fibrinogen, because much intermediate water exists there.…”

Section: Observation Of Pmea/air and Pmea/water Interfacessupporting

confidence: 85%

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

Tanaka

Kobayashi

Murakami

et al. 2019

Bulletin of the Chemical Society of Japan

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During this period, he received a fellowship from the Japan Society for the Promotion of Science (JSPS Postdoctoral Fellowships). He worked as a researcher at Japan Science and Technology Agency (JAT) ERATO Project and Kyushu Synchrotron Light Research Center. He moved to Kyushu University as an Assistant Professor in 2015. His current research activities are concerned with the analysis of structure, properties, and hydration state of biomaterial interfaces.

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Section: Observation Of Pmea/air and Pmea/water Interfacessupporting

confidence: 85%

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

Tanaka

Kobayashi

Murakami

et al. 2019

Bulletin of the Chemical Society of Japan

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“…Polymers often exhibit their functions in water, or in the presence of water, that is not just a solvent but plays an important role in the expression of functions. The water molecules related to those functions exist in the vicinity of the polymers and, compared to bulk water, have different properties, which have been studied by various techniques such as differential scanning calorimetry (DSC), 1 , 2 neutron reflectometry, 3 − 5 atomic force microscopy, 6 and contact angle measurements. 7 , 8 Vibrational spectroscopy has been used to analyze the structural and dynamical properties of aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

Infrared Spectra and Hydrogen-Bond Configurations of Water Molecules at the Interface of Water-Insoluble Polymers under Humidified Conditions

et al. 2022

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Elucidating the state of interfacial water, especially the hydrogen-bond configurations, is considered to be key for a better understanding of the functions of polymers that are exhibited in the presence of water. Here, an analysis in this direction is conducted for two water-insoluble biocompatible polymers, poly(2-methoxyethyl acrylate) and cyclic(poly(2-methoxyethyl acrylate)), and a non-biocompatible polymer, poly( n -butyl acrylate), by measuring their IR spectra under humidified conditions and by carrying out theoretical calculations on model complex systems. It is found that the OH stretching bands of water are decomposed into four components, and while the higher-frequency components (with peaks at ∼3610 and ∼3540 cm –1 ) behave in parallel with the C=O and C–O–C stretching and CH deformation bands of the polymers, the lower-frequency components (with peaks at ∼3430 and ∼3260 cm –1 ) become pronounced to a greater extent with increasing humidity. From the theoretical calculations, it is shown that the OH stretching frequency that is distributed from ∼3650 to ∼3200 cm –1 is correlated to the hydrogen-bond configurations and is mainly controlled by the electric field that is sensed by the vibrating H atom. By combining these observed and calculated results, the configurations of water at the interface of the polymers are discussed.

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“…To understand how IW contributes to the blood compatibility of the hydrated polymers, several studies have investigated the interfacial structure of PMEA analogues at the polymer/water interface. − Regular nanometer-scale protrusions were found at the PMEA analogue/water interfaces, while irregular large aggregates were observed at the interface between nonblood compatible polymers such as poly( n -butyl acrylate) (PBA) and water. − The protruded interfacial structure was suggested to be a phase separation of the polymer and water in the interface region resulting from the partial entanglement of polymers with the bulk polymer phase. , The protrusions (called polymer-rich regions) of both blood- and nonblood-compatible polymers adsorbed more fibrinogen than the flat regions (called water-rich regions) . In addition, the fibrinogens, which were adsorbed on the surface of blood-compatible polymers, exhibited less denaturation behavior and easily desorbed from the surface in comparison with those on the surface of nonblood-compatible polymers. , Therefore, water molecules in the water-rich regions have been suggested to have a capacity for preventing fibrinogen adsorption and denaturation, and IW molecules would play an important role in this preferred characteristic. , …”

Section: Introductionmentioning

confidence: 99%

Molecular Dynamics Study on the Water Mobility and Side-Chain Flexibility of Hydrated Poly(ω-methoxyalkyl acrylate)s

Kuo¹,

Urata²,

Koguchi³

et al. 2020

ACS Biomater. Sci. Eng.

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Intermediate water (IW) is known to play an important role in the antifouling property of biocompatible polymers. However, how IW prevents protein adsorption is still unclear. To understand the role of IW in the antifouling mechanism, molecular dynamics simulation was used to investigate the dynamic properties of water and side-chains for hydrated poly(ω-methoxyalkyl acrylate)s (PMCxA, where x indicates the number of methylene carbons) with x = 1–6 and poly(n-butyl acrylate) (PBA) in this study. Since the polymers uptake more water than their equilibrium water content (EWC) at the polymer/water interface, we analyzed the hydrated polymers at a water content higher than that of EWC. It was found that the water molecules interacting with one polymer oxygen atom (BW1), of which most are IW molecules, in PMC2A exhibit the lowest mobility, while those in PBA and PMC1A show a higher mobility. The result was consistent with the expectation that the biocompatible polymer with a long-resident hydration layer possesses good antifouling property. Through the detailed analysis of side-chain binding with three different types of BW1 molecules, we found that the amount of side-chains simultaneously interacting with two BW1 molecules, which exhibit the highest flexibility among the three kinds of side-chains, is the lowest for PMC1A. The high mobility of BW1 is thus suggested as the main factor for the poor protein adsorption resistance of PMC1A even though it possesses enough IW content and relatively flexible side-chains. Contrarily, a maximum amount of side-chains simultaneously interacting with two BW1 molecules was found in the hydrated PMC3A. The moderate side-chain length of PMC3A allows side-chains to simultaneously interact with two BW1 molecules and minimizes the hydrophobic part attractively interacting with a protein at the polymer/water interface. The unique structure of PMC3A may be the reason causing the best protein adsorption resistance among the PMCxAs.

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Analysis of Interaction Between Interfacial Structure and Fibrinogen at Blood-Compatible Polymer/Water Interface

Cited by 27 publications

References 38 publications

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

Design of Polymeric Biomaterials: The “Intermediate Water Concept”

Infrared Spectra and Hydrogen-Bond Configurations of Water Molecules at the Interface of Water-Insoluble Polymers under Humidified Conditions

Molecular Dynamics Study on the Water Mobility and Side-Chain Flexibility of Hydrated Poly(ω-methoxyalkyl acrylate)s

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