Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus

Huang, Ailin; Wu, Tao; Wu, Xiuyun; Zhang, Biao; Shen, Yuanyuan; Wang, Suying; Song, Wei; Ruan, Haihua

doi:10.3389/fmicb.2021.752791

Cited by 4 publications

(7 citation statements)

References 44 publications

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“…The extracts were centrifuged at 4,500 rpm for 30 min, and the supernatant was filtered through a Minisart R filter with a pore size of 0.22 μm for later use. The HPLC detection conditions for adenosine and HEA were consistent with the previous studies ( Huang et al, 2021 ). The adenosine and HEA content in mycelium was measured in mg/g, with “g” representing the dry weight of the mycelium.…”

Section: Methodssupporting

confidence: 76%

“…The C. cicadae strain AH 10-4 with higher yield of HEA was used in this study, which was isolated and purified in our laboratory in the previous study ( Huang et al, 2021 ). Cordyceps cicadae stain AH 10-4 was plated to PDA medium and incubated at 26°C for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…Three 25 mm 2 PDA cultures were punched out by the inoculation spatula and then transferred to the seed medium. The seed culture was then grown in a 250-mL flask containing 100 mL of its medium (per liter: glucose 20 g, peptone 10 g, yeast extract 10 g, KH 2 PO 4 2 g, MgSO 4 0.5 g, ZnSO 4 0.5 g, and pH natural) at 26°C and 140 rpm for 2 days ( Huang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Since then, adenosine and HEA have become important indicators for assessing the quality of Cordyceps products. A wild cicada flowers from Anji, Zhejiang city in China was isolated and purified in our laboratory ( Huang et al, 2021 ), which was identified as a new strain of C. cicadae by phylogenetic tree with higher yield of adenosine and HEA as compared to previous studies ( Chunyu et al, 2019 ; Qu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Zhu,

Ruan,

et al. 2024

Front. Microbiol.

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Cordyceps cicadae, as a new food ingredient, is a valuable edible and medicinal fungi. However, its resources are severely depleted due to environmental limitations and excessive harvesting practices. N6-(2-hydroxyethyl) adenosine (HEA), as an important product of Cordyceps cicadae, has the potential to be used in medical industry due to its diverse disease curing potential. However, the disclosure of HEA synthesis still severely limited its application until now. In this study, the kinetic curves for adenosine and HEA under shaker fermentation were explored. The kinetics of HEA and adenosine production exhibited a competitive pattern, implicating a possibility of sharing a same step during their synthesis. Due to HEA as a derivative of nitrogen metabolism, the effect of different nitrogen sources (peptone, yeast extract, ammonium sulfate, diammonium oxalate monohydrate, ammonium citrate dibasic, and ammonium citrate tribasic) on HEA production in Cordyceps cicadae strain AH 10-4 had been explored under different incubation conditions (shaker fermentation, stationary fermentation, and submerged fermentation). Our results indicated that the complex organic nitrogen sources were found to improve the accumulation of HEA content under shaker fermentation. In contrast, the optimal nitrogen source for the accumulation of HEA under stationary fermentation and submerged fermentation was ammonium citrate tribasic. But submerged fermentation obviously shortened the incubation time and had a comparable capacity of HEA accumulation by 2.578 mg/g compared with stationary fermentation of 2.535 mg/g, implicating a possibility of scaled-up production of HEA in industry by submerged fermentation. Based on the dramatic HEA production by ammonium sulfate as nitrogen resources between stationary and shaker fermentations, alanine, aspartate and glutamate as well as arginine metabolic pathway were related to the production of HEA by comparative transcriptome. Further investigation indicated that glutamic acid, which is an analog of Asp, showed an optimum production of HEA in comparison with other amino acids.

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Section: Methodssupporting

confidence: 76%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Zhu,

Ruan,

et al. 2024

Front. Microbiol.

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“…It is known as chan hua in Chinese, or “cicada flower”. The fungus grows throughout the nymph and destroys it, forming fruiting bodies that sprout from the nymph’s head [ 2 , 3 ]. It has been used as a TCM ingredient for many years [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Poisoning from Ingestion of Fungus-Infected Cicada Nymphs: Characteristics and Clinical Outcomes of Patients in Thailand

Trakulsrichai,

Satsue,

Tansuwannarat

et al. 2023

Toxins

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The current data regarding poisoning associated with ingestion of fungus-infected cicada nymphs are limited. We performed a retrospective cohort study of patients who ingested fungus-infected cicada nymphs and were referred to the Ramathibodi Poison Center for consultation from June 2010 to June 2022. Thirty-nine patients were included for analysis. Most were men (53.8%). Mean age was 40.2 ± 15.0 years. All nymphs were ingested as a health/food supplement. Thirty-one patients (79.5%) reported gastrointestinal symptoms. Median time from ingestion to symptom onset was 5 h. Twenty-nine patients (74.4%) reported neurological symptoms, including tremor, myoclonus, muscle rigidity, nystagmus/ocular clonus, drowsiness, dysarthria, seizure, and confusion. Some complained of dizziness, urinary retention, and jaw stiffness. Most patients (94.9%) were admitted to the hospital. Median hospital stay was 3 days. Ibotenic acid was detected in the blood and urine samples of one patient. All received supportive care. Four patients developed infectious complications. No deaths occurred. Consuming fungus-infected cicada nymphs may cause poisoning in humans. Gastrointestinal and neurological symptoms were common. Ibotenic acid might be the underlying cause. The main treatment is supportive care and appropriate management of complications. Education of the general public is advocated to prevent the incidence of this type of poisoning.

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Gut microbiome insights from 16S rRNA analysis of 17-year periodical cicadas (Hemiptera: Magicicada spp.) Broods II, VI, and X

Brumfield

Raupp

Haji

et al. 2022

Sci Rep

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Periodical cicadas (Hemiptera: Magicicada) have coevolved with obligate bacteriome-inhabiting microbial symbionts, yet little is known about gut microbial symbiont composition or differences in composition among allochronic Magicicada broods (year classes) which emerge parapatrically or allopatrically in the eastern United States. Here, 16S rRNA amplicon sequencing was performed to determine gut bacterial community profiles of three periodical broods, including II (Connecticut and Virginia, 2013), VI (North Carolina, 2017), and X (Maryland, 2021, and an early emerging nymph collected in Ohio, 2017). Results showed similarities among all nymphal gut microbiomes and between morphologically distinct 17-year Magicicada, namely Magicicada septendecim (Broods II and VI) and 17-year Magicicada cassini (Brood X) providing evidence of a core microbiome, distinct from the microbiome of burrow soil inhabited by the nymphs. Generally, phyla Bacteroidetes [Bacteroidota] (> 50% relative abundance), Actinobacteria [Actinomycetota], or Proteobacteria [Pseudomonadota] represented the core. Acidobacteria and genera Cupriavidus, Mesorhizobium, and Delftia were prevalent in nymphs but less frequent in adults. The primary obligate endosymbiont, Sulcia (Bacteroidetes), was dominant amongst core genera detected. Chryseobacterium were common in Broods VI and X. Chitinophaga, Arthrobacter, and Renibacterium were common in Brood X, and Pedobacter were common to nymphs of Broods II and VI. Further taxonomic assignment of unclassified Alphaproteobacteria sequencing reads allowed for detection of multiple copies of the Hodgkinia 16S rRNA gene, distinguishable as separate operational taxonomic units present simultaneously. As major emergences of the broods examined here occur at 17-year intervals, this study will provide a valuable comparative baseline in this era of a changing climate.

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Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus

Abstract: Graphical AbstractBrief strategy for wild cicada flowers internal and external microorganism community analysis and its applications.

Cited by 4 publications

References 44 publications

Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Exploiting the roles of nitrogen sources for HEA increment in Cordyceps cicadae

Poisoning from Ingestion of Fungus-Infected Cicada Nymphs: Characteristics and Clinical Outcomes of Patients in Thailand

Gut microbiome insights from 16S rRNA analysis of 17-year periodical cicadas (Hemiptera: Magicicada spp.) Broods II, VI, and X

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