Analysis of Interphase Nuclei

Schwarzacher, H. G.

doi:10.1007/978-3-642-65787-0_9

Cited by 4 publications

(3 citation statements)

References 50 publications

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“…However, simultaneous demonstration of the Barr body and of Y chornatin as a complementary diagnosis to prenatal chromosomal analysis is justified because of its simplicity. This view, expressed by Klinger & Moser (1972) and Murkherje et al (1974), is strengthened by Schwarzacher (1976), who claims that standardization of the method of fixation would lead to comparable results between various laboratories. For Barr-body demonstration, however, he recommends, in addition, later refixation of the preparations with 96 % alcohol.…”

Section: Discussionmentioning

confidence: 97%

Sex determination in nuclei of amnion fluid cells

1978

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Hypotonic treatment of amnion fluid cell-nuclei was found to enhance demonstration of Y chromatin and of autosomal fluorescence in interphase cell-nuclei following quinacrine mustard staining. In contrast, visualization of the Barr body was not improved by this treatment. Nuclear sex determination on cellular material from 200 samples of amniotic fluid was unequivocal in 197 cases; in only 3 cases were the findings uncertain. Therc were no erroneous determinations. In one instance maternal cellular tissue was observcd in smear preparations. Nuclear sex determination presents a simple method of supplementing and substantiating prenatal chromosomal diagnosis.

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Section: Discussionmentioning

confidence: 97%

Sex determination in nuclei of amnion fluid cells

1978

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“…After G-band staining, a very fine banding pattern can be observed (UNAKUL et at., 1973;SCHWARZACHER et at., 1974). As has been already pointed out, there are many more bands to be seen than even in prophase chromosomes, but the bands are of certain minimum size and are divided by negatively stained regions (Figs.…”

Section: Premature By Condensed Chromosomesmentioning

confidence: 99%

“…83). But even electron microscopy of prematurely condensed chromosomes in S-phase has so far not succeeded in demonstrating the replicating DNA molecule (SCHWARZACHER et a!., 1974). This has been called "fragmentation", but it resembles the condensed chromosome parts which are not replicating, whereas the replicating DNA is situated between the condensed parts (RAO and JOHNSON, 1974).…”

Section: Premature By Condensed Chromosomesmentioning

confidence: 99%

Chromosomes

Schwarzacher¹

1976

Handbuch Der Mikroskopischen Anatomie Des Menschen

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H-Y antigen expression in a case of XX true hermaphroditism

Moreira-Filho

Otto

Mustacchi³

et al. 1980

Hum Genet

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Cells from an XX true hermaphrodite expressed a reduced amount of H-Y antigen when compared with normal XY cells and with cells from his father, who had an XY/XX chromosomal constitution. His mother had a normal karyotype and was H-Y negative. The four brothers of the patient were clinically and karyotypically normal. An X-Y interchange followed by random inactivation of the X chromosome is proposed to explain the H-Y antigen titer found in the patient.

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Analysis of Interphase Nuclei

Cited by 4 publications

References 50 publications

Sex determination in nuclei of amnion fluid cells

Sex determination in nuclei of amnion fluid cells

Chromosomes

H-Y antigen expression in a case of XX true hermaphroditism

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