Analysis of intestinal microbial communities of cerebral infarction and ischemia patients based on high throughput sequencing technology and glucose and lipid metabolism

Ji, Wenzhen; Zhu, Yulan; Kan, Pengcheng; Cai, Ying; Wang, Zhida; Wu, Zijian; Yang, Ping

doi:10.3892/mmr.2017.7227

Cited by 46 publications

(35 citation statements)

References 25 publications

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“…Benakis et al found that the microbiota dysbiosis would affect the ischemic stroke outcome via suppressing the traffick of effector T cells from the gut to the leptomeninges in middle cerebral artery occlusion model [9]. Though only a few studies have investigated the role of microbiota in stroke patient, dysregulation of the microbiota in patients following stroke have been identified [12–14]. Yin et al, found that ischemic stroke was associated with different microbiota structure and more opportunistic pathogens, such as Megasphaera , Enterobacter , Oscillibacter , along with fewer commensal or beneficial genera such as Prevotella , Bacteroides , and Faecalibacterium [13].…”

Section: Discussionmentioning

confidence: 99%

Change of intestinal microbiota in cerebral ischemic stroke patients

et al. 2019

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Background Gut microbiota has been suggested to play a role in stroke patients. Nevertheless, little is known about gut microbiota and the clinical indexes in stroke patients. Methods Total of 30 cerebral ischemic stroke (CI) patients and 30 healthy control were enrolled in this study and the fecal gut microbiota was profiled via Illumina sequencing of the 16S rRNA V1-V2. The National Institutes of Health Stroke Scale (NIHSS) were used to quantify stroke severity and modified Rankin scale (mRS) to assess outcome for CI patients. The correlations between the clinical indexes and microbiota were evaluated. Results Though the microbial α-diversity and structure is similar between CI patients and healthy controls, the gut microbiota of CI patients had more short chain fatty acids producer including Odoribacter , Akkermansia, Ruminococcaceae_ UCG_005 and Victivallis . We also found that the special microbes were correlation with serum index, such as norank_O _ _Mollicutes_RF9 , Enterobacter , Ruminococcaceae _UCG-002 were negative correlation with LDL (r = − 0.401, P < 0.01), HDL (r = − 0.425, P < 0.01) and blood glucose (r = − 0.439, P < 0.001), while the HDL was significantly positive correlation with the genus Ruminococcus _1 (r = 0.443, P < 0.001). The Christensenellaceae _R-7_group and norank_f_ Ruminococcaceae was significantly positive correlation with NIHSS1M (r = 0.514, P < 0.05; r = 0.449, P < 0.05) and mRS (r = 0.471, P < 0.05, r = 0.503, P < 0.01), respectively. On the other hand, the genus Enterobacter was significantly negative correlation with NIHSS1M (r = 0.449, P < 0.05) and mRS (r = 0.503, P < 0.01). Conclusions This study suggests that CI patients showed significant dysbiosis of the gut microbiota with enriched short chain fatty acids producer, including Odoribacter , Akkermansia . This dysbiosis was correlation with the outcomes and deserves further study. Electronic supplementary material The online version of this article (10.1186/s12866-019-1552-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Change of intestinal microbiota in cerebral ischemic stroke patients

et al. 2019

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“…Our previous study showed that ApoE levels in the serum of patients with CI were significantly higher than those in the healthy group. [ 16 , 17 ] Additionally, there was a correlation between ApoE and Bacteroidia and Gammaproteobacteria. Bacteroidia and Gammaproteobacteria are the major microorganisms in the human intestine and play important roles in lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

The characteristics analysis of intestinal microecology on cerebral infarction patients and its correlation with apolipoprotein E

Wang¹,

Xu²,

Yao

et al. 2018

Medicine

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Cerebral infarction (CI) is associated with high rates of disability, mortality, and death in China, but its mechanism is unclear. Therefore, early diagnosis of CI and determining its mechanism are very important. Intestinal microecology is thought to be related to cardiovascular and cerebrovascular diseases. We hypothesized that intestinal microecology is also related to CI and that the intestinal microecology in the stool of CI patients differs from that in healthy people.Fecal samples of healthy subjects and CI patient (all n = 10) and we investigated the intestinal microecology of CI patient and healthy people stool by 16 seconds sequencing and analyzed relative abundance and diversity of microorganisms by unweighted pair-group method with arithmetic mean analysis (UPGMA) and principal co-ordinates analysis (PCoA). We also measured apolipoprotein E (ApoE) levels in the serum by ELISA assay and analyzed the correlation between ApoE and intestinal flora.We found that the relative structure and diversity of intestinal microecology was significantly different between the stools of CI patients and healthy people. At the class level, Gammaproteobacteria was increased and Bacteroidia was decreased in CI patient stool. We found a correlation between ApoE in the serum and Bacteroidia and Gammaproteobacteria species.We considered the intestinal flora can be used as an indicator of CI and the up-regulation of ApoE may be the potential mediate for intestinal microecology contribute to CI.

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“…Composition of the microbiome is related with incidence of obesity, diabetes, certain cancers, diseases of the intestine and cardiovascular system, and kidneys pathologies [2][3][4][5][6][7][8]. It has been also shown that a number of diseases, for example, stroke and chronic kidney disease, in turn, lead to alterations in the composition of the microbiota, creating a "vicious circle" [9,10]. Some bacteria can reduce the barrier function of the mucous membrane by producing special lipopolysaccharides and proteases [11].…”

Section: Introductionmentioning

confidence: 99%

Microbiome-Metabolome Signature of Acute Kidney Injury

et al. 2020

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Intestinal microbiota play a considerable role in the host’s organism, broadly affecting its organs and tissues. The kidney can also be the target of the microbiome and its metabolites (especially short-chain fatty acids), which can influence renal tissue, both by direct action and through modulation of the immune response. This impact is crucial, especially during kidney injury, because the modulation of inflammation or reparative processes could affect the severity of the resulting damage or recovery of kidney function. In this study, we compared the composition of rat gut microbiota with its outcome, in experimental acute ischemic kidney injury and named the bacterial taxa that play putatively negative or positive roles in the progression of ischemic kidney injury. We investigated the link between serum creatinine, urea, and a number of metabolites (acylcarnitines and amino acids), and the relative abundance of various bacterial taxa in rat feces. Our analysis revealed an increase in levels of 32 acylcarnitines in serum, after renal ischemia/reperfusion and correlation with creatinine and urea, while levels of three amino acids (tyrosine, tryptophan, and proline) had decreased. We detected associations between bacterial abundance and metabolite levels, using a compositionality-aware approach—Rothia and Staphylococcus levels were positively associated with creatinine and urea levels, respectively. Our findings indicate that the gut microbial community contains specific members whose presence might ameliorate or, on the contrary, aggravate ischemic kidney injury. These bacterial taxa could present perspective targets for therapeutical interventions in kidney pathologies, including acute kidney injury.

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Analysis of intestinal microbial communities of cerebral infarction and ischemia patients based on high throughput sequencing technology and glucose and lipid metabolism

Cited by 46 publications

References 25 publications

Change of intestinal microbiota in cerebral ischemic stroke patients

Change of intestinal microbiota in cerebral ischemic stroke patients

The characteristics analysis of intestinal microecology on cerebral infarction patients and its correlation with apolipoprotein E

Microbiome-Metabolome Signature of Acute Kidney Injury

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