Analysis of intracytoplasmic sperm injection procedures related to delayed insemination and ejaculated, epididymal and testicular spermatozoa

Andrews, MM; Fishel, S. B.; Rowe, Philip H.; Berry, JA; Lisi, Franco; Rinaldi, Leonardo

doi:10.1016/s1472-6483(10)62231-6

Cited by 10 publications

(3 citation statements)

References 34 publications

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“…Microinjection after a minimum of one hour of in vitro matured human oocytes up to 8 hours after extrusion of the first polar body resulted in high fertilization rates and good embryo quality (11). Prolonged incubation of metaphase II oocytes to a maximum of 8 hours did not compromise fertilization and pregnancy rates (12)(13)(14)(15). The longest period of delay between retrieval and in vitro insemination without interfering outcome has been reported as 16 hours (16).…”

Section: Discussionmentioning

confidence: 98%

Birth after 12 hours of oocyte refrigeration

Coban¹,

Hacifazlioglu²,

Ciray³

et al. 2010

Fertility and Sterility

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Section: Discussionmentioning

confidence: 98%

Birth after 12 hours of oocyte refrigeration

Coban¹,

Hacifazlioglu²,

Ciray³

et al. 2010

Fertility and Sterility

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“…In rescue ICSI, the decrease in embryo development owing to oocyte aging associated with the delayed timing of fertilization has been a concern. The fertilization and pregnancy rates have reportedly reduced when the time from oocyte retrieval to ICSI was prolonged, 24 , 25 , 26 but it has also been reported that no effects were observed up to 9 h 27 and 12 h 28 ; as such, there is no consensus on the impact of delayed timing of ICSI implementation on embryo development. Studies on embryo development in early rescue ICSI have reported grades of cleavage stage embryos lower than IVF 20 or equal to ICSI, 8 , 17 but there are few studies on their development into blastocysts.…”

Section: Discussionmentioning

confidence: 99%

Usefulness of expanding the indications of early rescue intracytoplasmic sperm injection

Shibahara¹,

Fukasaku²,

Miyazaki³

et al. 2021

Reprod Medicine & Biology

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Purpose Early rescue intracytoplasmic sperm injection (ICSI) is often performed in cases in which not even a single oocyte has extruded a second polar body 6 h after insemination. We evaluated the usefulness of expanding the indications of early rescue ICSI to cases in which <80% of oocytes have extruded second polar bodies 6 h after insemination. Methods Early rescue ICSI was performed on oocytes that were denuded 2.5 h post‐insemination and whose extrusion of the second polar bodies had been examined 6 h post‐insemination with a PolScope. Results In vitro fertilization was performed on 24 496 oocytes of 4944 cycles, and 1438 cycles had <80% rate of the second polar body extrusion. Rescue ICSI was performed on 3933 oocytes. Three pronuclei (3PN) incidence of rescue ICSI was 3.0% in oocytes with ≥50% rate of the second polar body extrusion. With respect to the second polar body extrusion rate, no differences were observed in normal fertilization, blastocyst development, implantation, miscarriage, or live birth rates for rescue ICSI. Conclusion By expanding the indications of early rescue ICSI using the PolScope to cases in which <80% of oocytes have extruded the second polar bodies, many fertilized oocytes can be obtained without considerably increasing the 3PN rate.

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“…Indeed, successful fertilization may occur over a wide timeframe. Trounson et al [6] believed that a delay of 5-6.5 hours before insemination was required for maximizing the incidence of fertilization and pregnancy, which we now know is erroneous-successful fertilization may occur immediately after ovulation or much later without compromising results [7].) It remained for another 10 years following the Shettles paper for the inquiry of Edwards finally to elucidate and document precisely the detailed events of human oocyte maturation following release from the follicle: germinal vesicle breakdown up to 24 hours; diakinesis duration, 25-28 hours; metaphase-1, 36-43 hours; and metaphase-2 and polar body release, 36-43 hours (8).…”

Section: Ex Ovo Omniamentioning

confidence: 99%

First in vitro fertilization baby—this is how it happened

Fishel¹

2018

Fertility and Sterility

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On July 25, 1978, the first human was born following extracorporeal fertilization, an event that opened up a new medical science: expanding our knowledge of and developing novel treatments for infertility, radically changing the opportunities for families with inherited monogenic disorders, generating the new discipline of clinical embryology, and paving the way for studies into stem cell biology. In vitro fertilization (IVF), as it became known in its simplest form, went even further: it engaged the myriad of minds in human society. Not only were books written on the moral status of the human embryo, the ethics of IVF practice, and exercising governments on appropriate-which turned out to be disparate-regulation, it redefined family life! The prediction I made in 1985 that one day we may see five "parents" for one child became a reality quicker than we could have imagined at the time. The new medical science marched inexorably on in almost all countries of the world-a universal human plight at last had an opportunity for remedy: more than 10 million couples seeking a resolution to their infertility became parents, men who had no option to have their own genetic child became genetic fathers, and ever-increasing monogenic conditions were not being passed on to the next generation. The future may well bring to bear the opportunity for in vitro-developed viable gametes to generate successful pregnancies, and other "futuristic" opportunities for IVF science. But its story began over a century ago with seeking an understanding on how an egg matures and how to achieve successful fertilization-a fundamental scientific inquiry. It took one man to go beyond that scientific endeavor, to take head on a society unprepared and unwilling to accept human fertilization in vitro and unempathetic to the plight of the infertile; one man to see what prospects lay ahead for humanity should IVF become a reality, and for that man to battle every step of the way for nearly 2 decades to achieve that dream.

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Analysis of intracytoplasmic sperm injection procedures related to delayed insemination and ejaculated, epididymal and testicular spermatozoa

Cited by 10 publications

References 34 publications

Birth after 12 hours of oocyte refrigeration

Birth after 12 hours of oocyte refrigeration

Usefulness of expanding the indications of early rescue intracytoplasmic sperm injection

First in vitro fertilization baby—this is how it happened

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