Analysis of intragenic recombination at<i>wx</i>in rice: Correlation between the molecular and genetic maps within the locus

Inukai, Tsuyoshi; Sako, Aya; Hirano, Hiroyuki; Sano, Yoshio

doi:10.1139/g00-015

Cited by 61 publications

(53 citation statements)

References 37 publications

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“…Both amino acid residues were conserved among the six waxy protein sequences derived from wheat, barley, maize, potato, pea and rice (Ainsworth et al 1993). Inukai et al (2000) characterized the M8F (74-8) mutant, which is one of the four wx leaky mutants screened by Amano (1985), at the molecular level and one amino acid substitution (IIe-182 to Asn-182 change) was also found in exon 5. This residue is conserved between the five plant species except for pea (Ainsworth et al 1993).…”

Section: Discussionmentioning

confidence: 98%

Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.).

et al. 2002

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In this paper, we characterized the Wx-mq gene for low amylose content in a rice variety, Milky Queen, at the molecular level. The Wx-mq gene was cloned by RT-PCR, and a nearly full-length cDNA sequence of the gene was determined. Sequence comparison between the Wx-mq gene and the wild type allele (Wx-b), cloned from cv. Koshihikari, revealed that two base changes existed within the coding region; a G to A base change at nucleotide position 497 and a T to C base change at nucleotide position 595. Each nucleotide substitution should generate a missense base change (an Arg-158 to His-158 change in exon4, and a Tyr-191 to His-191 change in exon5). However, it is not known which missense mutation is essential for the activity of the WX protein. To identify rice varieties and lines, which harbored the Wx-mq gene, PCR primers were designed at the gene level. These primers were able to amplify the Wx-mq specific 741 bp band in Milky Queen, and in other rice variety and lines, Milky Princess, Joiku 436 and Etsunan 190, all of which have the same pedigree as that of Milky Queen. On the other hand, no 741 bp band was amplified with the primers in Koshihikari which harbored the wild type allele (Wx-b), and the other low-amylose content variety and line, Snow Pearl and NM391, which do not have the pedigree. Thus, it is possible to detect the Wx-mq gene by PCR.

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Section: Discussionmentioning

confidence: 98%

Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.).

et al. 2002

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“…The latter observation is not surprising given the high degree of intergenic gross structural polymorphism in maize (36,47). It will be interesting to examine the genome-wide breakdown of recombination in rice, a grass species with intragenic recombination hotspots (48), like maize, but with much less repetitive DNA (49).…”

Section: Discussionmentioning

confidence: 99%

Polarized gene conversion at the bz locus of maize

Dooner

2014

Proc. Natl. Acad. Sci. U.S.A.

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Nucleotide diversity is greater in maize than in most organisms studied to date, so allelic pairs in a hybrid tend to be highly polymorphic. Most recombination events between such pairs of maize polymorphic alleles are crossovers. However, intragenic recombination events not associated with flanking marker exchange, corresponding to noncrossover gene conversions, predominate between alleles derived from the same progenitor. In these dimorphic heterozygotes, the two alleles differ only at the two mutant sites between which recombination is being measured. To investigate whether gene conversion at the bz locus is polarized, two large diallel crossing matrices involving mutant sites spread across the bz gene were performed and more than 2,500 intragenic recombinants were scored. In both diallels, around 90% of recombinants could be accounted for by gene conversion. Furthermore, conversion exhibited a striking polarity, with sites located within 150 bp of the start and stop codons converting more frequently than sites located in the middle of the gene. The implications of these findings are discussed with reference to recent data from genome-wide studies in other plants.meiotic recombination | conversion polarity

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“…In recent years, this concept seems to be changing because of more evidence demonstrating that intragenic recombination may also play an important role in gene evolution [46, see 42-45 for reviews] and the maintenance of population genetic variability in eukaryotes [47][48][49][50]. Higher intragenic recombination rates (recombination hotspots) than the genome average have been observed at a number of gene loci in plants, such as the wx locus in rice (Oryza sativa) [51] and maize (Zea mays) [52], the maize A1 locus [53], and the maize bronze locus [54,55]. However, this seems not to be the case in Arabidopsis.…”

Section: Intragenic Recombinationmentioning

confidence: 99%

“…High recombination rates have been observed at some of the non-disease resistance gene loci in plants [51][52][53][54][55]. Molecular characterization of the maize R gene complex identified recombinant alleles derived from the intragenic unequal exchange between the genes in this complex [70].…”

Section: Intragenic Recombinationmentioning

confidence: 99%

Meiosis-Driven Genome Variation in Plants

Cai¹,

Xu²

2007

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Meiosis includes two successive divisions of the nucleus with one round of DNA replication and leads to the formation of gametes with half of the chromosomes of the mother cell during sexual reproduction. It provides a cytological basis for gametogenesis and inheritance in eukaryotes. Meiotic cell division is a complex and dynamic process that involves a number of molecular and cellular events, such as DNA and chromosome replication, chromosome pairing, synapsis and recombination, chromosome segregation, and cytokinesis. Meiosis maintains genome stability and integrity over sexual life cycles. On the other hand, meiosis generates genome variations in several ways. Variant meiotic recombination resulting from specific genome structures induces deletions, duplications, and other rearrangements within the genic and non-genic genomic regions and has been considered a major driving force for gene and genome evolution in nature. Meiotic abnormalities in chromosome segregation lead to chromosomally imbalanced gametes and aneuploidy. Meiotic restitution due to failure of the first or second meiotic division gives rise to unreduced gametes, which triggers polyploidization and genome expansion. This paper reviews research regarding meiosis-driven genome variation, including deletion and duplication of genomic regions, aneuploidy, and polyploidization, and discusses the effect of related meiotic events on genome variation and evolution in plants. Knowledge of various meiosis-driven genome variations provides insight into genome evolution and genetic variability in plants and facilitates plant genome research.

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Analysis of intragenic recombination atwxin rice: Correlation between the molecular and genetic maps within the locus

Cited by 61 publications

References 37 publications

Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.).

Molecular Characterization of Wx-mq, a Novel Mutant Gene for Low-amylose Content in Endosperm of Rice (Oryza sativa L.).

Polarized gene conversion at the bz locus of maize

Meiosis-Driven Genome Variation in Plants

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