Analysis of isoflavones in Puerariae radix by high-performance liquid chromatography with amperometric detection

Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography

“…These isoflavones distribute in fractions of different polarity. The contents determined are consistent with those published [30].…”

Rapid Simultaneous Determination of Major Isoflavones of Pueraria lobata and Discriminative Analysis of its Geographical Origins by Principal Component Analysis

“…In all samples, puerarin was the most abundant compound while the amounts of daidzin, genistin and daidzein were low. This was consistent with that reported earlier (Kitada et al , ), where puerarin, daidzin and daidzein within a commercial Radix Puerariae sample from Japan were studied simultaneously by using HPLC and amperometric detection. The amounts of the four isoflavones varied from sample to sample to different extent (Table ).…”

“…For example, puerarin and daidzein in PLO were determined by capillary electrophoresis (CE; Chen et al, 2001;Cao et al, 2002). HPLC was also used successfully in the quantification of RPI (Kitada et al, 1985;Prasain et al, 2003;Zhang et al, 2005). However, a single HPLC run usually takes 1 h or longer, and the time and mobile phase consumption can be problematic.…”

Separation and identification of isoflavones with on‐line liquid chromatography‐electrochemistry‐thermospray mass spectrometry

“…The isoflavones are a group of plant metabolites based on the structure of 3-phenyl-4H-l-benzopyran-4-one ( Figure 1). They have been identified in clovers [ 1-31, cowpea (41, alfalfa [ 51, Pueranhe radix [6] and soybeans [ 7-91. They are of primary interest as a result of their estrogenic and fungitoxic properties.…”

“…These have generally used linear gradient solvent systems utilizing a mixture of either methanol or acetronitrile and a neutral or acidic buffer, such as ammonium acetate or phosphate [2,4,[16][17][18][19]. Also, isocratic solvent systems have been described [6,8,9,21,22 In recent decades electrochemical methods in combination with spectroscopy (spectroelectrochemistry) and/ or liquid chromatography (LCEC) have resulted in significant contributions to the study of redox mechanisms and metabolic pathways of many biological molecules [23, 241. The combination of analytical techniques often results in more useful information than can be obtained from each technique used alone.…”