Analysis of lamprey meis genes reveals that conserved inputs from Hox, Meis and Pbx proteins control their expression in the hindbrain and neural tube

Parker, Hugo J.; Kumar, Bony De; Pushel, Irina; Bronner, Marianne E.; Krumlauf, Robb

doi:10.1016/j.ydbio.2021.07.014

Cited by 6 publications

(4 citation statements)

References 96 publications

(167 reference statements)

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“…The other genes also regulate development [24][25][26][27]. Hmx interacts with Gsx in development of the brain's hypothalamus [28].…”

Section: Resultsmentioning

confidence: 99%

DNA conserved in diverse animals since the Precambrian controls genes for embryonic development

Frith

2023

Preprint

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How does one fertilized cell develop into an exquisitely complex animal? Complex development evolved via simple development, and retains ancient components: genes that control development in similar ways in distantly-related animals, like humans and flies. Expression of these genes is controlled by DNA segments near the genes, but these segments seem less widely conserved. Arguably, just four have been found conserved between vertebrates and non-deuterostome animals. Here are reported twenty-five regulatory DNA segments conserved across bilaterian animals, of which seven are conserved in cnidaria (coral and sea anemone). They control developmental genes (e.g. Nr2f, Ptch, Rfx1/3, Sall, Smad6, Sp5, Tbx2/3), including six homeobox genes: Gsx, Hmx, Meis, Msx, Six1/2, and Zfhx3/4. The segments contain perfectly or near-perfectly conserved CCAAT boxes, E-boxes, and other sequences recognized by regulatory proteins. More such DNA conservation will surely be found soon, as more genomes are published and sequence comparison is optimized. This reveals a control system for animal development conserved since the Precambrian.

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“…The other genes also regulate development [24][25][26][27]. Hmx interacts with Gsx in development of the brain's hypothalamus [28].…”

Section: Resultsmentioning

confidence: 99%

DNA conserved in diverse animals since the Precambrian controls genes for embryonic development

Frith

2023

Preprint

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“…The other genes also regulate development ( Lorente-Sorolla et al 2018 ; Robinton et al 2019 ; Guntur et al 2021 ; Parker et al 2021 ). Hmx interacts with Gsx in development of the brain’s hypothalamus ( Wang et al 2004 ).…”

Section: Resultsmentioning

confidence: 99%

DNA Conserved in Diverse Animals Since the Precambrian Controls Genes for Embryonic Development

Frith,

2023

Molecular Biology and Evolution

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DNA that controls gene expression (e.g. enhancers, promoters) has seemed almost never to be conserved between distantly related animals, like vertebrates and arthropods. This is mysterious, because development of such animals is partly organized by homologous genes with similar complex expression patterns, termed “deep homology.” Here, we report 25 regulatory DNA segments conserved across bilaterian animals, of which 7 are also conserved in cnidaria (coral and sea anemone). They control developmental genes (e.g. Nr2f, Ptch, Rfx1/3, Sall, Smad6, Sp5, Tbx2/3), including six homeobox genes: Gsx, Hmx, Meis, Msx, Six1/2, and Zfhx3/4. The segments contain perfectly or near-perfectly conserved CCAAT boxes, E-boxes, and other sequences recognized by regulatory proteins. More such DNA conservation will surely be found soon, as more genomes are published and sequence comparison is optimized. This reveals a control system for animal development conserved since the Precambrian.

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“…Next, we focused on Hoxa2 , which is widely considered a key determinant of the hyoid arch development. Hoxa2 has been reported to be directly regulated by MEIS-PBX complex in zebrafish and lamprey ( Parker et al, 2019 , 2021 ) and MEIS2 protein is strongly expressed in the E10.5 hyoid arch ( Fabik et al, 2020 ). However, we found no perceivable change in the expression of Hoxa2 in E10.5 Meis2 cKOs (3/3) ( Figure 9A–B′ ).…”

Section: Resultsmentioning

confidence: 99%

Meis2 controls skeletal formation in the hyoid region

Fabik

Psutkova

Machoň

2022

Front. Cell Dev. Biol.

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A vertebrate skull is composed of many skeletal elements which display enormous diversity of shapes. Cranial bone formation embodies a multitude of processes, i.e., epithelial-mesenchymal induction, mesenchymal condensation, and endochondral or intramembranous ossification. Molecular pathways determining complex architecture and growth of the cranial skeleton during embryogenesis are poorly understood. Here, we present a model of the hyoid apparatus development in Wnt1-Cre2-induced Meis2 conditional knock-out (cKO) mice. Meis2 cKO embryos develop an aberrant hyoid apparatus—a complete skeletal chain from the base of the neurocranium to lesser horns of the hyoid, resembling extreme human pathologies of the hyoid-larynx region. We examined key stages of hyoid skeletogenesis to obtain a complex image of the hyoid apparatus formation. Lack of Meis2 resulted in ectopic loci of mesenchymal condensations, ectopic cartilage and bone formation, disinhibition of skeletogenesis, and elevated proliferation of cartilage precursors. We presume that all these mechanisms contribute to formation of the aberrant skeletal chain in the hyoid region. Moreover, Meis2 cKO embryos exhibit severely reduced expression of PBX1 and HAND2 in the hyoid region. Altogether, MEIS2 in conjunction with PBX1 and HAND2 affects mesenchymal condensation, specification and proliferation of cartilage precursors to ensure development of the anatomically correct hyoid apparatus.

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Analysis of lamprey meis genes reveals that conserved inputs from Hox, Meis and Pbx proteins control their expression in the hindbrain and neural tube

Abstract: Analyses of lamprey meis genes reveals that conserved inputs from hox, Meis and Pbx proteins control their expression in the hindbrain and neural tube, Developmental Biology (2021), doi:

Cited by 6 publications

References 96 publications

DNA conserved in diverse animals since the Precambrian controls genes for embryonic development

DNA conserved in diverse animals since the Precambrian controls genes for embryonic development

DNA Conserved in Diverse Animals Since the Precambrian Controls Genes for Embryonic Development

Meis2 controls skeletal formation in the hyoid region

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