Analysis of laser-induced plasmid DNA photolysis by capillary electrophoresis

Quantitative Analysis of Plasmid Forms by Agarose and Capillary Gel Electrophoresis

“…Furthermore, the authors' assumptions that migration time is a substance-specific constant and that the peak area represents the concentration could not be proven. We also may not follow the previous assignment of plasmid forms to CGE peaks (12)(13)(14).…”

“…Only one clear band will occur if the tested plasmid DNA was treated with a single cutting restriction enzyme. Determination of plasmid forms by CGE has been reported earlier (12)(13)(14). While the authors described the separation of monomeric forms, the various dimeric concatamers that are very often found in plasmid preparations were not considered.…”

Analysis of plasmid DNA damage induced by melanin with capillary electrophoresis

“…Several methods have been employed to induce the plasmid cleavage in order to obtain the different topological forms of plasmid DNA. Nackerdien et al have used laser irradiation to get the various forms of the plasmids [22]. Mao et al have applied a special restriction endonuclease to get the isomers of the plasmids [26].…”

“…Unlike linear ds-DNA separations, where a number of different polymers have been developed and tested in CE with varying degrees of success [20], little attention was paid to the use of matrices for plasmids DNA separation, although the electrophoretic behavior of plasmids differs greatly from the behavior of familiar and widely studied linear DNA [21]. In the sparse literature on CE of plasmid DNA only few matrices such as polyacrylamide [22], hydroxyethyl cellulose [1,21,23,24], hydroxypropylmethyl cellulose [18,25,26] and polydimethylacrylamide [27] are used for successful separations. Most of these studies have employed coated capillaries to suppress electroosmotic flow.…”

The use of capillary electrophoresis with entangled polymer matrix to analyse plasmid DNA and a cationic lipid/cholesterol liposome: DNA complex

“…This paper describes the use of CE with an entangled polymer matrix for the separation of conformational isomers in pDNA of approximately 9000 base pairs. Whilst separations of this type have been previously documented [21 ] this paper focuses on practical applications of the technique. The method was also successfully applied to the characterisation of pDNAwithin the Complex.…”