Analysis of lead distribution in avian organs by LA-ICP-MS: Study of experimentally lead-exposed ducks and kites

Torimoto, Ryouta; Ishii, Chikako; Sato, Hiroshi; Saito, Keisuke; Watanabe, Yukiko; Ogasawara, Kohei; Kubota, Ayano; Matsukawa, Tetsuya; Yokoyama, Kazuhito; Kobayashi, Atsushi; Kimura, Takashi; Nakayama, Shouta M.M.; Ikenaka, Yoshinori; Ishizuka, Mayumi

doi:10.1016/j.envpol.2021.117086

Cited by 9 publications

(2 citation statements)

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“…Based on the available literature, low laser energy conditions and a relatively high scanning rates are recommended for LA-ICP-MS when analysing thin slides of biological tissues polymer embedded [ 14 , 18 , 21 , 31 , 32 ]. The use of soft ablation conditions by scan line as an ablation work mode is specially needed when analytes such as NPs must be imaged since longer periods of time laser incidence and too high laser energy would lead to NP ionisation resulting in a continuous signal (smoother discrete signals) [ 21 ] instead of well-defined and recognised peaks in the continuous LA-ICP-MS record.…”

Section: Resultsmentioning

confidence: 99%

Quantitative titanium imaging in fish tissues exposed to titanium dioxide nanoparticles by laser ablation-inductively coupled plasma-mass spectrometry

et al. 2023

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Imaging studies by laser ablation–inductively coupled plasma mass spectrometry have been successfully developed to obtain qualitative and quantitative information on the presence/distribution of titanium (ionic titanium and/or titanium dioxide nanoparticles) in sea bream tissues (kidney, liver, and muscle) after exposure assays with 45-nm citrate-coated titanium dioxide nanoparticles. Laboratory-produced gelatine standards containing ionic titanium were used as a calibration strategy for obtaining laser ablation–based images using quantitative (titanium concentrations) data. The best calibration strategy consisted of using gelatine-based titanium standards (from 0.1 to 2.0 μg g−1) by placing 5.0-μL drops of the liquid gelatine standards onto microscope glass sample holders. After air drying at room temperature good homogeneity of the placed drops was obtained, which led to good repeatability of measurements (calibration slope of 4.21 × 104 ± 0.39 × 104, n = 3) and good linearity (coefficient of determination higher than 0.990). Under the optimised conditions, a limit of detection of 0.087 μg g−1 titanium was assessed. This strategy allowed to locate prominent areas of titanium in the tissues as well as to quantify the bioaccumulated titanium and a better understanding of titanium dioxide nanoparticle spatial distribution in sea bream tissues. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Quantitative titanium imaging in fish tissues exposed to titanium dioxide nanoparticles by laser ablation-inductively coupled plasma-mass spectrometry

et al. 2023

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“…Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an elemental imaging technique in which small sections of laser-ablated tissues are sequentially introduced into ICP-MS to create a grid image of metal concentrations (Becker et al, 2014;Qin et al, 2011). LA-ICP-MS has recently been used in various studies to investigate the renal distribution of metals, such as mercury (Hg) (Iwase et al, 2021;Liu et al, 2021), lead (Torimoto et al, 2021), and platinum (Pt) (Egger et al, 2014;Legin et al, 2016;Moreno-Gordaliza et al, 2011). Many studies have shown that the renal toxicity of heavy metals, such as Hg, Pt, Cd, and chromium, is manifested in a region-specific manner, i.e., some metals induce more pronounced damage in the S1 and S2 regions of the proximal tubules while others do so in the S3 regions (Dorian et al, 1992;Ichimura et al, 2004;Zalups et al, 2014;Zhang et al, 2008;Zhou et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Spatial localization of cadmium and metallothionein in the kidneys of mice at the early phase of cadmium accumulation

et al. 2022

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Chronic exposure to cadmium (Cd) leads to an accumulation of Cd in the kidneys. Metallothionein (MT) is a low-molecular-weight protein having a high affinity for Cd. Cd bound to MT in serum is filtered through the glomeruli of kidney nephrons and reabsorbed by endocytosis into the proximal tubules from the luminal side. Accumulation of Cd in renal cells induces MT synthesis, leading to long-term deposition of Cd and the suppression of Cd toxicity. Recently, many studies have investigated the tissue distribution of metals using laser ablation ICP-MS (LA-ICP-MS). However, little information has been available regarding renal Cd distribution. Hence, we dually investigated the renal distribution of Cd by LA-ICP-MS and that of MT by immunohistochemistry to clarify the dose-and time-dependent changes in the distributions of Cd and MT in mice exposed to Cd from drinking water for 1, 2, and 4 months. Both Cd and MT exhibited characteristic heterogeneous distribution patterns in the renal cortex. The accumulation of Cd and MT near the surface of the cortex suggests a preferential accumulation of Cd in the surface nephrons. MT distribution was more pronounced in the proximal tubules than in the distal tubules, and there were clear differences in MT immunostaining even among the proximal tubules. The heterogeneous localization of MT may reflect the nephron-specific accumulation of Cd. Combining elemental imaging of Cd with immunostaining of MT proved a successful strategy to reveal the characteristic renal Cd distribution, especially in the early stages of Cd accumulation.

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Consequences of Developmental Exposure to Pollution: Importance of Stress-Coping Mechanisms

Angelier

2022

Development Strategies and Biodiversity

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Analysis of lead distribution in avian organs by LA-ICP-MS: Study of experimentally lead-exposed ducks and kites

Cited by 9 publications

References 62 publications

Quantitative titanium imaging in fish tissues exposed to titanium dioxide nanoparticles by laser ablation-inductively coupled plasma-mass spectrometry

Quantitative titanium imaging in fish tissues exposed to titanium dioxide nanoparticles by laser ablation-inductively coupled plasma-mass spectrometry

Spatial localization of cadmium and metallothionein in the kidneys of mice at the early phase of cadmium accumulation

Consequences of Developmental Exposure to Pollution: Importance of Stress-Coping Mechanisms

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