Analysis of legumain and cystatin 6 expression at the maternal-fetal interface in pigs

Shim, Ji-Yeon; Seo, Heewon; Choi, Yohan; Yoo, Inkyu; Lee, Chang Kyu; Hyun, Sang‐Hwan; Lee, Eunsong; Ka, Hakhyun

doi:10.1002/mrd.22192

Cited by 16 publications

(7 citation statements)

References 31 publications

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“…Interestingly, the genes LGMN and CTSB, which play important roles in the endosomal processing of endocytosed antigens, were down-regulated. The LGMN gene encodes a cysteine protease that presents bacterial and endogenous peptides to major histocompatibility complex (MHC) class II molecules in the lysosomal and endosomal systems [ 39 ]. The CTSB protein is a lysosomal cysteine proteinase that is comprised of a dimer of disulfide-linked heavy and light chains that are made from the same protein precursor that is involved in proteolytic processing [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs

et al. 2015

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A long-term high-energy diet affects human health and leads to obesity and metabolic syndrome in addition to cardiac steatosis and hypertrophy. Ectopic fat accumulation in the heart has been demonstrated to be a risk factor for heart disorders, but the molecular mechanism of heart disease remains largely unknown. Bama miniature pigs were fed a high-fat, high-sucrose diet (HFHSD) for 23 months. These pigs developed symptoms of metabolic syndrome and showed cardiac steatosis and hypertrophy with a greatly increased body weight (2.73-fold, P<0.01), insulin level (4.60-fold, P<0.01), heart weight (1.82-fold, P<0.05) and heart volume (1.60-fold, P<0.05) compared with the control pigs. To understand the molecular mechanisms of cardiac steatosis and hypertrophy, nine pig heart cRNA samples were hybridized to porcine GeneChips. Microarray analyses revealed that 1,022 genes were significantly differentially expressed (P<0.05, ≥1.5-fold change), including 591 up-regulated and 431 down-regulated genes in the HFHSD group relative to the control group. KEGG analysis indicated that the observed heart disorder involved the signal transduction-related MAPK, cytokine, and PPAR signaling pathways, energy metabolism-related fatty acid and oxidative phosphorylation signaling pathways, heart function signaling-related focal adhesion, axon guidance, hypertrophic cardiomyopathy and actin cytoskeleton signaling pathways, inflammation and apoptosis pathways, and others. Quantitative RT-PCR assays identified several important differentially expressed heart-related genes, including STAT3, ACSL4, ATF4, FADD, PPP3CA, CD74, SLA-8, VCL, ACTN2 and FGFR1, which may be targets of further research. This study shows that a long-term, high-energy diet induces obesity, cardiac steatosis, and hypertrophy and provides insights into the molecular mechanisms of hypertrophy and fatty heart to facilitate further research.

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Section: Discussionmentioning

confidence: 99%

Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs

et al. 2015

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“…Expression of CTSs and CSTs in the uterine endometrium has been shown in many species including rodents, humans, ruminants and pigs [ 50 – 52 ], and it has been suggested that CTSs and CSTs play important roles in endometrial remodeling in the uterine endometrium during the reproductive cycle and pregnancy [ 52 ]. In pigs, CTSB, CTSL1, and LGMN are expressed by endometrial luminal epithelial and chorionic epithelial cells at the maternal-conceptus interface during pregnancy and endometrial expression of CTSB and CTSL1 is induced by progesterone [ 51 , 53 ], suggesting that their action is required to remodel uterine endometrial and placental tissues and facilitate transplacental transport of nutrients. CST6 is expressed in the uterine endometrium during the estrous cycle and in pregnancy, and also in the chorionic epithelia of the placental membrane with increasing levels during late pregnancy [ 53 ], suggesting that cell type-specific expression and function of CST6 is critical in regulation of CTS action for appropriate maternal-fetal interactions.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Stage-Specific Gene Expression Profiles in the Uterine Endometrium during Pregnancy in Pigs

et al. 2015

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The uterine endometrium plays a critical role in regulating the estrous cycle and the establishment and maintenance of pregnancy in mammalian species. Many studies have investigated the expression and function of genes in the uterine endometrium, but the global expression pattern of genes and relationships among genes differentially expressed in the uterine endometrium during gestation in pigs remain unclear. Thus, this study investigated global gene expression profiles using microarray in pigs. Diverse transcriptome analyses including clustering, network, and differentially expressed gene (DEG) analyses were performed to detect endometrial gene expression changes during the different gestation stages. In total, 6,991 genes were found to be differentially expressed by comparing genes expressed on day (D) 12 of pregnancy with those on D15, D30, D60, D90 and D114 of pregnancy, and clustering analysis of detected DEGs distinguished 8 clusters. Furthermore, several pregnancy-related hub genes such as ALPPL2, RANBP17, NF1B, SPP1, and CST6 were discovered through network analysis. Finally, detected hub genes were technically validated by quantitative RT-PCR. These results suggest the complex network characteristics involved in uterine endometrial gene expression during pregnancy and indicate that diverse patterns of stage-specific gene expression and network connections may play a critical role in endometrial remodeling and in placental and fetal development to establish and maintenance of pregnancy in pigs.

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“…Cathepsin L1 (CTSL1) was localized to chorionic epithelia that form areolae, which absorb secretions from uterine glands. Message for the cysteine endopeptidase LGMN and its inhibitor CST6 localized with strong intensity in both the luminal epithelium (LE) and glandular epithelium (GE) as well as in the chorionic epithelium by day 30 of pregnancy in pigs (Shim et al, 2013). In SCNT pregnancies, both LGMN and CST6 were misregulated in the endometrium underlying the placenta compared to IVV pregnancies.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of PigIn Vivo,In Vitro–Fertilized, and Nuclear Transfer Blastocyst-Stage Embryos Treated with Histone Deacetylase Inhibitors Postfusion and Activation Reveals Changes in the Lysosomal Pathway

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Mao

Lee

et al. 2015

Cellular Reprogramming

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Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated with suberoylanilide hydroxamic acid (SAHA), 4-iodo-SAHA (ISAHA), or Scriptaid as compared to untreated SCNT, in vitro-fertilized (IVF), and in vivo (IVV) blastocyst-stage embryos. SAHA (10 μM) had the highest level of blastocyst development at 43.9%, and all treatments except 10 μM ISAHA had the same percentage of blastocyst development as Scriptaid (p<0.05). Two treatments, 1.0 μM ISAHA and 1.0 μM SAHA, had higher mean cell number than No HDACi treatment (p<0.021). Embryo transfers performed with 10 μM SAHA- and 1 μM ISAHA-treated embryos resulted in the birth of healthy piglets. GenBank accession numbers from up- and downregulated transcripts were loaded into the Database for Annotation, Visualization and Integrated Discovery to identify enriched biological themes. HDACi treatment yielded the highest enrichment for transcripts within the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway, lysosome. The mean intensity of LysoTracker was lower in IVV embryos compared to IVF and SCNT embryos (p<0.0001). SAHA and ISAHA can successfully be used to create healthy piglets from SCNT.

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Analysis of legumain and cystatin 6 expression at the maternal-fetal interface in pigs

Cited by 16 publications

References 31 publications

Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs

Global Transcriptomic Profiling of Cardiac Hypertrophy and Fatty Heart Induced by Long-Term High-Energy Diet in Bama Miniature Pigs

Analysis of Stage-Specific Gene Expression Profiles in the Uterine Endometrium during Pregnancy in Pigs

Transcriptome Analysis of PigIn Vivo,In Vitro–Fertilized, and Nuclear Transfer Blastocyst-Stage Embryos Treated with Histone Deacetylase Inhibitors Postfusion and Activation Reveals Changes in the Lysosomal Pathway

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