Analysis of Limbal Stem Cell Deficiency by Corneal Impression Cytology

Donisi, Pietro Maria; Rama, Paolo; Fasolo, Adriano; Ponzin, Diego

doi:10.1097/00003226-200308000-00009

Cited by 109 publications

(87 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3,4 Nevertheless, corneal impression cytology is not a routine clinical procedure in contact lens practice. Donissi and colleagues, 4 concluded that impression cytology could have an important role in evaluating patients undergoing therapeutic penetrating keratoplasty to select those who would benefit from limbal stem cell transplantation. Impression cytology is essential to confirm if these patients have LSCD induced by contact lens wear.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3] Patients with LSCD can present with a range of symptoms, including irritation, photophobia and decreased visual acuity. 3,4 The diagnosis relies on laboratory tests such as impression cytology to detect the presence of goblet cells or the expression of certain types of keratin in corneal epithelium. 3,4 Corneal diseases that manifest LSCD can be subdivided into two major categories.…”

mentioning

confidence: 99%

“…3,4 The diagnosis relies on laboratory tests such as impression cytology to detect the presence of goblet cells or the expression of certain types of keratin in corneal epithelium. 3,4 Corneal diseases that manifest LSCD can be subdivided into two major categories. 5 In the first, limbal epithelial stem cells are destroyed by known or recognisable agents, such as a chemical or thermal burn, Stevens-Johnson syndrome, toxic epidermal necrolysis, multiple ocular surgeries or long-term topical medications (iatrogenic), 6 severe microbial infection, radiation, anti-metabolites including 5-fluorouracil and mitomycin C, and contact lens wear.…”

mentioning

confidence: 99%

“…The underlying pathophysiology explains why transplantation of epithelial stem cells and restoration of the limbal stem cell stromal environment (for example, by amniotic membrane transplantation or limbal transplantation) are necessary for ocular surface reconstruction in cases of severe LSCD. 2 Contact lens-induced LSCD may represent 15 per cent of LSCD 4 but there are only clinical reports describing LSCD secondary to contact lens wear. 1 We present clinical characteristics of a series of long-standing contact lens patients with corneal conjunctivalisation without a specific disease known to cause LSCD.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Corneal conjunctivalisation in long‐standing contact lens wearers

Martín

2007

Clinical and Experimental Optometry

View full text Add to dashboard Cite

Background: There are many contact lens-related ocular surface disorders. Some can damage the limbal region where stem cells are thought to be located in its basal cell layer. This damage can result in destruction and a deficiency of corneal stem cells. One important sign of this complication is corneal conjunctivalisation. The purpose of this study is to describe clinical characteristics of a series of long-standing contact lens (CL) wearers with corneal conjunctivalisation (CC). Results: There were 24 eyes of 14 myopic patients (93 per cent women) with CC without a specific disease entity known to cause limbal stem cell deficiency (LSCD). Conjunctivalisation occurred in the inferior limbus of three eyes (12 per cent). Only four patients (28.6 per cent) reported previous ocular symptoms. All were myopic with a mean spherical equivalent of -8.80 ± 5.00 (SD) dioptres (range from -1.75 to -21.50 D) and mean visual acuity 0.9 ± 0.2 (range from 0.4 to 1.2). The mean years of CL wear was 17.6 ± 8.5 (CI 95% 13.2 to 22; range six to 30). All were daily-wear patients with a mean daily-wear time of 12.5 ± 1.8 hours per day (CI 95% 11.6 to 13.4).

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Corneal conjunctivalisation in long‐standing contact lens wearers

Martín

2007

Clinical and Experimental Optometry

View full text Add to dashboard Cite

show abstract

“…Las alteraciones a nivel del limbo producen importantes trastornos a nivel del la superficie del epitelio corneal que pueden ser detectados mediante la citología de impresión (18). En este trabajo planteamos un estudio trasversal para determinar las características citológicas del epitelio corneal en diferentes entidades etiológicas que tienen en común la presencia de un cuadro de deficiencia limbal.…”

Section: Discussionunclassified

Determinación del grado de metaplasia escamosa del epitelio corneal como factor diagnóstico de insuficiencia limbal

López-García

Rivas

García-Lozano³

2006

Arch Soc Esp Oftalmol

View full text Add to dashboard Cite

RESUMENObjetivos: Determinar si existe relación entre el grado de metaplasia escamosa de la superficie corneal mediante citología de impresión y la gravedad clínica en pacientes con insuficiencia limbal. Métodos: Hemos estudiado mediante citología de impresión 98 ojos de pacientes con insuficiencia limbal que fueron divididos en cuatro grupos según la gravedad clínica: 14 ojos no presentaban sintomatología alguna, 34 ojos presentaron un grado leve de insuficiencia limbal, 28 ojos un grado moderado y 22 ojos una insuficiencia limbal grave. A todos los pacientes se les realizó una citología corneal en la que se estudió el tamaño celular, tamaño nuclear, alteraciones nucleares y relación núcleo-citoplasma (N:C), así como la presencia de células caliciformes. Resultados: Los pacientes con insuficiencia limbal asintomática presentaron un tamaño celular medio de 477 DE 140 µm 2 , con una relación N:C de 1:5,25 DE 1,5. Los pacientes con insuficiencia limbal leve presentaron un tamaño celular medio de 764 DE 122,6 µm 2 y una relación N:C de 1:8,2 DE 1,4. Nin- ARTÍCULO ORIGINAL ABSTRACTPurpose: To determine the correlation between the squamous metaplasia grade of the corneal surface and the clinical severity of the limbal deficiency in patients with this disorder. Methods: We studied 98 eyes of patients with limbal deficiency by impression cytology. These patients were divided into four groups in relation to their clinical severity: 14 eyes had no symptoms, 34 eyes showed a mild grade of limbal deficiency, 28 eyes had a moderate grade and 22 eyes had severe limbal deficiency. Corneal cytology was performed in each patient. Cellular size, nuclear size, nuclear changes and the nuclear-cytoplasmic (N:C) ratio were defined in corneal epithelial cells, in addition to evaluation for the presence of goblet cells in the corneal epithelium. Results: In patients with limbal deficiency without symptoms, we found that the cellular size was 477 (SD140) µm 2 and the N:C ratio was 1:5.25 (SD1.5). In patients with mild limbal deficiency, the cellular size was 764 (SD122.6) µm 2 and the N:C ratio was 1:8.2 (SD1.4). These patients did not show corneal

show abstract

MUC16 as a Sensitive and Specific Marker for Epithelial Downgrowth

Pai

Glasgow²

2010

Arch Ophthalmol

View full text Add to dashboard Cite

Analysis of Limbal Stem Cell Deficiency by Corneal Impression Cytology

Cited by 109 publications

References 22 publications

Corneal conjunctivalisation in long‐standing contact lens wearers

Corneal conjunctivalisation in long‐standing contact lens wearers

Determinación del grado de metaplasia escamosa del epitelio corneal como factor diagnóstico de insuficiencia limbal

MUC16 as a Sensitive and Specific Marker for Epithelial Downgrowth

Contact Info

Product

Resources

About