Analysis of Lingolytic Enzymes and Decolorization of Disperse Violet S3rl, Yellow Brown S2rfl, Red W4bs, Yellow SRLP and Red S3b by Brown Rot Fungi

Abstract

Current study was designed to investigate the ability of Coniophora puteana IEBL-1, Daedalea dickinsii IEBL-2, Piptoporus betulinus IEBL-3 and Fimitopsis pinicola IEBL-4 to decolorized disperse textile dyes. Five disperse dyes, purchased from supplier were studied including; disperse violet S3RL, yellow brown S2RFL, red W4BS, yellow SRLP and red S3B. The decolorization process was observed for 10 consecutive days with the analysis of process on each day. The results showed that D. dickinsii IEBL-2 (70-80 %) has the more potential of biodegradation of disperse dyes while P. betulinus IEBL-3 (47-59 %) has the least. The study of lignolytic enzymes i.e. lignin peroxidase, manganese peroxidase and laccase showed that C. puteana IEBL-1 and D. dickinsii IEBL-2 produced most active enzymes. Higher enzymatic activities related with more degradation indicated that these are involved in this process. Enzymes showed maximum activities at 30 0 C and pH 6.5 with good affinity towards their substrates as indicated by kinetic values. The result indicates that these BRF isolates are suitable for the treatment of textile wastewater.

Archives of Environmental Protection

“…It was reported earlier by Mahmood et al 2017 that C. puteana IEBL-1 has a very good ability of biodegradation of various disperse textile dyes during screening of different brown rot fungi for their potential. In continuation of that study, followed by successful optimization and enhancement of biodegradation of textile dyes by this fungus, the potential was tested on real industrial effl uents.…”

Physicochemical parameters optimization and peroxidase characterization from Aspergillus niger native strain by solid-state fermentation for improved dye decolorization

“…The purified enzyme Km value was 126 µM with a reaction velocity of 537 UmL -1 with the substrate (guaiacol). A. niger POX with H2O2 and guaiacol as a substrate had values of Km obtained which were 0.751 mM for Li and Mn POX while Vmax values were 1000 to 1250 µM/mL (Mahmood et al 2017). Similarly, the value of purified Trametes versicolor laccase Km of 73 µM and 780 U/mL Vmax was obtained using 2,2'-azinobis [3-ethylbenzothiazoline-6-sulfonic acid]diammonium salt as substrate (Asgher et al 2012).…”

“…POX catalyzes the detoxification of substrates with hydrogen peroxide as an electron acceptor that is widely distributed in plants, animals, fungi, bacteria, and mammalian cells (Chen et al 2023). POX had wide applications as animal fodder, contaminants breakdown, wastewater treatment, dye decolorization, bioethanol production, and for lignin breakdown in pulp and paper industry applications (Bansal and Kanwar 2013;Mahmood et al 2017;Liu et al 2020;Xu et al 2021). Enhanced POX activity has been reported for Anthracophyllum discolor with optimized media conditions having KH2PO4 and Tris/HCl with a pH of 5.5 at 50 °C (Acevedo et al 2011).…”

First Report on the Bioremediation of Textile Industrial Effluents by Piptoporus Betulinus IEBL-3 by Using Response Surface Methodology

“…Biodegradation of textile effluents: Considering the dyes biodegradation potential of P. betulinus IEBL-3 [12] the biodegradation of industrial effluents was optimized in the current study for expanding its industrial applications. For this, 90 mL of effluent was taken in a 250 mL flask, mixed with 10 mL broth media and fungal inoculum, and the effluent concentration was maintained as mentioned in Table 1.…”