Analysis of lipid hydroperoxides

Dobarganes, M. C.; Velasco, Joaquı́n

doi:10.1002/1438-9312(200207)104:7<420::aid-ejlt420>3.0.co;2-n

Cited by 124 publications

(95 citation statements)

References 56 publications

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“…Peroxides are the main initial products of lipid oxidation and can be measured by iodometric methods (AOCS, 2004), techniques based on iron oxidation (SAFETY ASSOCIATES, 1998;, enzymatic reactions, or by physical or chromatographic methods (DOBARGANES; VELASCO, 2002).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the quality of pet foods using fast techniques and official methods

Osawa¹,

Gonçalves²,

Ragazzi³

2008

Ciênc. Tecnol. Aliment.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the quality of pet foods using fast techniques and official methods

Osawa¹,

Gonçalves²,

Ragazzi³

2008

Ciênc. Tecnol. Aliment.

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“…Routine analysis techniques to estimate oxidative stress in biological samples include 46 iodometric assays [9], spectrophotometric determination of conjugated dienes [7,10], 47 avoid oxidation (unoxidized rat plasma, URP). Plasma samples used to determine the 108 precision and recovery of the method were stored in the dark at 4ºC and analyzed within 24h 109 after extraction (fresh URP).…”

Section: Introduction 34mentioning

confidence: 99%

Determination of total plasma hydroperoxides using a diphenyl-1-pyrenylphosphine fluorescent probe

Santas

Guardiola

Rafecas

et al. 2013

Analytical Biochemistry

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19Plasma hydroperoxides (HP) are widely accepted to be good indicators of oxidative stress. By 20 means of the method proposed herein, which uses diphenyl-1-pyrenylphosphine (DPPP) as a 21 fluorescent probe, all types of plasma HP were determined. The detection and quantification 22 limits of the method were 0.08 and 0.25 nmols of cumene hydroperoxide (CHP) equivalents 23 in 40 µL of plasma, respectively. The method is satisfactory in terms of precision (5.3% for 24 14.5 µM CHP eq.; n=8) and the recoveries were 91% and 92% after standard additions of 26 25 and 52 µM of CHP, respectively. The selectivity of the proposed method is higher than 96%. 26Moreover, optimization of the reaction conditions and the addition of 27 ethylenediaminetetraacetic acid (EDTA) disodium salt and 2,6-di-tert-butyl-4-methylphenol 28 (BHT) prevented the formation of HP artifacts during the analysis. Therefore, the proposed 29 method is useful for simple and quantitative determination of total plasma HP. 30 31

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“…The latter is especially useful for small samples and has the advantage of being nondestructive (Dobarganes and Velasco, 2002).…”

Section: Introductionmentioning

confidence: 99%

Relationship between changes in peroxide value and conjugated dienes during oxidation of sunflower oils with different degree of unsaturation

Marmesat¹,

Morales²,

Velasco³

et al. 2009

Grasas y Aceites

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RESUMENRelaciones entre los cambios en el índice de peróxi-dos e índice de dienos conjugados durante la oxidación de aceites de girasol con diferente grado de instauración.El índice de peróxidos y la medida de dienos conjugados mediante la extinción específica a 232 nm se utilizan alternativamente para la evaluación de compuestos primarios de oxidación en estudios de oxidación de aceites y grasas. El objetivo de este estudio fue definir las relaciones entre ambas medidas en aceites de girasol con diferente grado de insaturación. Los aceites estudiados fueron aceite de girasol convencional (SO) y aceite de girasol alto oleico (HOSO). Con fines comparativos, también se estudió el linoleato de metilo, cuyos hidroperóxidos presentan estructura de dienos conjugados. Las muestras fueron oxidadas a 40 °C en la oscuridad. Con el fin de acelerar la oxidación a 40 °C, se eliminaron los antioxidantes naturales de los aceites, y se aplicó una relación superficie/volumen de aceite elevada (0.8 cm Ϫ1) para garantizar total disponibilidad de aire. Los resultados obtenidos se ajustaron a regresiones lineales con elevados coeficientes de correlación (0.9988, 0.9991 y 0.9977 para el linoleato de metilo, SO y HOSO, respectivamente). Sin embargo, se encontraron diferencias significativas entre las pendientes de las rectas (0.0974, 0.0854 y 0.0503 para el linoleato de metilo, SO y HOSO, respectivamente), lo que indica la formación en los aceites de hidroperóxidos no conjugados formados a partir del ácido oleico, incluso en el aceite SO, de alto grado de insaturación. En consecuencia, sólo el índice de peróxidos sería un índice de confianza para la evaluación de los hidroperóxidos en estudios de oxidación con aceites de diferente grado de insaturación, salvo que se utilicen rectas de calibración adecuadas. PALABRAS-CLAVE: Ácidos grasos -Dienos conjugadosHidroperóxidos -Índice de peróxidos -Linoleato de metiloOxidación. SUMMARYRelationship between changes in peroxide value and conjugated dienes during oxidation of sunflower oils with different degree of unsaturation.Peroxide value and the determination of conjugated dienes by UV absorption at 232 nm are normally applied alternatively for the evaluation of primary oxidation compounds. The objective of this study was to define the relationship between both indexes during oil oxidation in sunflower oils with different degree of unsaturation. The oils studied were conventional sunflower oil (SO) and high oleic sunflower oil (HOSO). For comparative purposes, methyl linoleate, whose hydroperoxides are conjugated diene hydroperoxides, was also studied. Samples were oxidized at 40 °C in the dark. In order to accelerate oxidation at 40 °C, the natural antioxidants present in the oils were removed, and a high surface to oil volume ratio (0.8 cm Ϫ1) was applied to guarantee total air availability. Linear regressions with high correlation coefficients between peroxide values and conjugated dienes were found for the three lipid systems studied (0.9988, 0.9991 and 0.9977 for methyl linoleate, SO an...

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Analysis of lipid hydroperoxides

Cited by 124 publications

References 56 publications

Evaluation of the quality of pet foods using fast techniques and official methods

Evaluation of the quality of pet foods using fast techniques and official methods

Determination of total plasma hydroperoxides using a diphenyl-1-pyrenylphosphine fluorescent probe

Relationship between changes in peroxide value and conjugated dienes during oxidation of sunflower oils with different degree of unsaturation

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