Analysis of Lipids by Gas Chromatography

Cruz‐Hernandez, Cristina; Destaillats, Frédéric

doi:10.1016/b978-0-12-385540-4.00023-7

Cited by 6 publications

(2 citation statements)

References 103 publications

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“…However, later-eluting compounds were not qualitatively analyzable by the GC-MS method due to several reasons. Different GC-MS methods need to be employed in order to reduce thermal stress of both eluting compounds and stationary phase, which results in a better identification. , Moreover, the Wiley10/NIST11 database alone is not able to provide a complete identification of all classes of extractives that could be found in lignocellulosic materials. This requires in-house solutions instead.…”

Section: Discussionmentioning

confidence: 99%

Tools for Bark Biorefineries: Studies toward Improved Characterization of Lipophilic Lignocellulosic Extractives by Combining Supercritical Fluid and Gas Chromatography

Barbini

Sriranganadane

Orozco

et al. 2020

ACS Sustainable Chem. Eng.

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The bark of trees contains an interesting mixture of bioactive compounds, or so-called extractives. The use of supercritical carbon dioxide (sc-CO2) eliminates both the need for organic solvents as extractants and the danger that solvent traces might compromise the purity of the extracts. Unfortunately, the complexity and natural variability of extracts’ composition render any utilization attempts rather challenging. Thus, in order to implement exploitation concepts in a meaningful way, appropriate analytical techniques for characterizing extracts must be available beforehand. In our work, we explored gas chromatography coupled to both mass spectrometry and a flame ionization detector (GC-MS/FID), in combination with ultraperformance convergence chromatography and quadrupole time-of-flight mass spectrometry (UPC2-QTof-MS), for the characterization of bark extracts from pine (Pinus sylvestris L.) in both qualitative and quantitative terms. Although the conventional GC-MS/FID approach is a robust method for overall quantification of extractives, it fails to provide ample information about native sterol esters and triglycerides. These data are provided by a new, complementary analytical technique based on supercritical carbon dioxide, as the chromatographic eluant, coupled to a high-resolution mass spectrometer. The combination of both techniques and the use of sc-CO2 as both an extraction solvent and eluant made this combined tool especially powerful. The most prominent triglycerides in the extract were identified qualitatively and quantitatively, and the dominating sterol esters were identified qualitatively, by UPC2-QTof-MS.

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Section: Discussionmentioning

confidence: 99%

Tools for Bark Biorefineries: Studies toward Improved Characterization of Lipophilic Lignocellulosic Extractives by Combining Supercritical Fluid and Gas Chromatography

Barbini

Sriranganadane

Orozco

et al. 2020

ACS Sustainable Chem. Eng.

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“…Flexible fused-silica columns coated with the highly polar cyanoalkyl polysiloxane stationary phase have been recommended for the analyses of FAME samples containing geometric and/or positional isomers of monounsaturated fatty acids and conjugated linoleic acid (CLA) isomers as well as a range of FA from butyric acid (4:0) to long chain polyunsaturated fatty acids (LC-PUFA, e.g., as present in milk or other animal tissues). These columns are generally available in various lengths up to 100 m from different suppliers and give satisfactory separations of samples containing fatty acids with different chain length (Cruz-Hernandez and Destaillats 2012;Cruz-Hernandez et al 2004;Wolff and Precht 2002). The 100 % cyanopropyl polysiloxane stationary phases are available as CP-Sil 88 (Agilent J&W Technologies, Palo Alto, CA), SP-2560 (Supelco Inc., Bellefonte, PA), and BPX-70 columns (SGE, Melbourne, Au).…”

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confidence: 99%

Gas Chromatography of Fatty Acid Methyl Esters: Resolution Using Conventional and High-Resolution Columns

Cruz‐Hernandez

Destaillats

2016

Encyclopedia of Lipidomics

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GC; FAME; GC columns No single method is currently available for separate fatty acids from complex mixture of natural (e.g., animal tissues such as milk, muscle, adipose tissue, or plasma) or modified lipids. However, a set of comprehensive analytical techniques could maximize the separation and quantification of most of the fatty acids. GC remains by far the most convenient method to identify and quantify fatty acid methyl esters (FAME). The nature of the stationary phase, column internal diameter, film thickness, and column length are important factors to select a proper column for specific application. Polar stationary phases are recommended for the separation of FAME. Packed and capillary (also known as open tubular) are the two main categories of columns. Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase. Most packed

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Advances in Analytical and Preparative Supercritical Fluid Chromatography. Food and Nutraceutical Applications

Montañés

Catchpole

Tallon

et al. 2014

Food Engineering Series

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Analysis of Lipids by Gas Chromatography

Cited by 6 publications

References 103 publications

Tools for Bark Biorefineries: Studies toward Improved Characterization of Lipophilic Lignocellulosic Extractives by Combining Supercritical Fluid and Gas Chromatography

Tools for Bark Biorefineries: Studies toward Improved Characterization of Lipophilic Lignocellulosic Extractives by Combining Supercritical Fluid and Gas Chromatography

Gas Chromatography of Fatty Acid Methyl Esters: Resolution Using Conventional and High-Resolution Columns

Advances in Analytical and Preparative Supercritical Fluid Chromatography. Food and Nutraceutical Applications

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