Analysis of Lipids by Thin-Layer Chromatography

Shantha, N.C.; Napolitano, Guillermo E.

doi:10.1016/s0301-4770(08)60307-3

Cited by 1 publication

(1 citation statement)

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“…Besides the aforementioned thin layer chromatographic techniques with varying detection reagents and principles for the semi-quantitative analysis of ether lipids and plasmalogens ( Schmid et al, 1975 ; Shantha and Napolitano, 1998 ), and other chromatographic approaches ( Christie and Han, 2012 ), a diversity of analytical approaches has been developed ( Messias et al, 2018 ). A series of assays based on radiolabeled substrates have been established, often to study the respective metabolic pathway structures and enzymology in a targeted manner ( Paltauf, 1972 ; Wykle et al, 1972 ; Blank and Snyder, 1992 ).…”

Section: History Of Ether Lipid Research and The Progress In Ether Li...mentioning

confidence: 99%

Tricky Isomers—The Evolution of Analytical Strategies to Characterize Plasmalogens and Plasmanyl Ether Lipids

Koch

Watschinger

Werner

et al. 2022

Front. Cell Dev. Biol.

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Typically, glycerophospholipids are represented with two esterified fatty acids. However, by up to 20%, a significant proportion of this lipid class carries an ether-linked fatty alcohol side chain at the sn-1 position, generally referred to as ether lipids, which shape their specific physicochemical properties. Among those, plasmalogens represent a distinct subgroup characterized by an sn-1 vinyl-ether double bond. The total loss of ether lipids in severe peroxisomal defects such as rhizomelic chondrodysplasia punctata indicates their crucial contribution to diverse cellular functions. An aberrant ether lipid metabolism has also been reported in multifactorial conditions including Alzheimer’s disease. Understanding the underlying pathological implications is hampered by the still unclear exact functional spectrum of ether lipids, especially in regard to the differentiation between the individual contributions of plasmalogens (plasmenyl lipids) and their non-vinyl-ether lipid (plasmanyl) counterparts. A primary reason for this is that exact identification and quantification of plasmalogens and other ether lipids poses a challenging and usually labor-intensive task. Diverse analytical methods for the detection of plasmalogens have been developed. Liquid chromatography–tandem mass spectrometry is increasingly used to resolve complex lipid mixtures, and with optimized parameters and specialized fragmentation strategies, discrimination between ethers and plasmalogens is feasible. In this review, we recapitulate historic and current methodologies for the recognition and quantification of these important lipids and will discuss developments in this field that can contribute to the characterization of plasmalogens in high structural detail.

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