This study presents a methodology used in developing the competitiveness improvement framework (CIF) for laboratories, in particular, Forensic Science Laboratories (FSLs). The cyclic nature of FSL processes allowed data collection for the purpose of identification of factors affecting FSL performance (cause factors). Flow charts were used to represent mathematical formulations for cause factor measurements and quantification of the baseline data on turnaround time (TAT), backlogs for case files (B g ), turnaround time in the supply chain (T sc ), and employee absenteeism (A b ). By quantifying the cause factors in addition to academic development coefficient (A d ) and complex longevity (L c ) for FSL employees, it was possible to establish the organizational design features requiring improvements. The relevance of cause factors to FSL stakeholders and means of improvement and sustainability were established. A detailed road map towards CIF was presented using D-MAIC methodology. The rated cause factors based on challenges in the FSL business environment were subjected to Pareto analysis to prioritize the challenges in order to improve FSLs' competitiveness. The interrelationship between the three dimensions of competitiveness improvement (process, performance and planning) was presented in terms of the affected six cause factors. Also, the potential lean practices for improving competitiveness of FSL based on measured cause factors have been presented. This paper introduced methods and measures for improving operational competitiveness of laboratories. The CIF was finally presented in a form of a series of three flow charts summarizing all steps implemented in its development with inputs and cause factors involved. Case-file backlogs were identified as one of the cause factors affecting the competitiveness of the (FSL). Backlogs represent case-files that remain unprocessed or unreported within a selected time interval (year, week or month) which leads to increased customer complaints, rework, cost of analysis, degradation of biological samples, etc. Case-file backlogging was quantified in three consecutive years (Y2014 to Y2016) to study variations as case files are processed [2] [3] [4] [5]. Data were collected for the case-files received and case-files processed, difference of which gives case-files backlogged. The time interval for a case-file to be regarded as backlogged was one week, which can translate into backlogged case-files per month or year. A data collection tool was established and used for three laboratory disciplines (forensic chemistry, biology/DNA and toxicology).