Analysis of metabolites in aqueous solutions by using laser Raman spectroscopy

Abstract

Laser Raman scattering is conducted on aqueous solutions that contain organic chemicals that include glucose, lactate, ascorbate, pyruvate, and urea. At the concentrations of interest (below 1.0 wt. %), these various metabolites are found to scatter light independently of each other, and the scattering is linearly proportional to their concentrations. Through proper subtraction of water background scattering, the spectrum that is due to metabolite scattering is obtained and the composition of the solution can be determined by fitting its Raman spectrum with a linear sum of the known pure metabolite spectra. The spectrum of rabbit aqueous humor is presented and the potential application of this analytical method, such as noninvasive glucose monitoring, is discussed.

Surface‐enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum

“…The potential of using Raman spectroscopy for biomolecules detection has been demonstrated. Wang et al [9] applied the Raman technique for metabolite analysis (glucose, lactic acid, pyruvate, urea) in aqueous solutions. Qu et al [10] detected multiple components (albumin, triglyceride, glucose) in serum using Raman spectroscopy.…”

Single‐Walled Carbon Nanotube Near‐Infrared Fluorescent Sensors for Biological Systems

“…In addition to fluorescence-based techniques, several photonics applications have also been proposed for glucose sensing [39,40]. However, the high-resolution gratings and notch filters necessary for Raman-based schemes are expensive [39,40].…”

“…However, the high-resolution gratings and notch filters necessary for Raman-based schemes are expensive [39,40]. A fluorescencebased device would require lower resolution and would be relatively inexpensive due to the affordability of current instrumentation and its amenability to microfabrication techniques [41].…”

Analysis of Near-infrared Raman Spectroscopy as a New Technique for a Transcutaneous Non-invasive Diagnosis of Blood Components

“…Several metabolites are being investigated, such as glucose, urea, cholesterol, lactic acid and triglyceride, and many others [18][19][20][21][22][23][24][25]. So far, e#orts are focused on the study of glucose content in blood, because of the huge number of diabetic patients for whom the monitoring of glucose is essential.…”