Analysis of Metoclopramide and Related Compounds in Tablets by Liquid Chromatography

Abstract

A rapid, sensitive, stability indicating, reversed phase HPLC method for the quantitation of Metoclopramide and its related compounds is described. injected on a reverse phase CIS column with a mobile phase of 0.15M ammonium acetate and acetonitrile (80:20) w i t h W detection at 268 nm. The method is highly specific, sensitive and has the ability to separate Metoclopramide narnely, 4-acetylamino-5-chloro-N[2(diethylamino)ethyll-2-methoxybenzamide hydrochloride and 4-amino-5-chloro-2methoxybenzoic acid in quantities of up to 10 ucg/mL. is highly reproducible with average assay recovery of 104.7 2 1.1%.The sample is extracted in methanol and from its related compunds;The method 1365 Copyright 0 1989 by Marcel Dekker, Inc. Drug Dev Ind Pharm Downloaded from informahealthcare.com by McMaster University on 11/03/14 For personal use only.

Novel molecularly imprinted polymers for the selective extraction and determination of metoclopramide in human serum and urine samples using high-performance liquid chromatography

“…Both the British Pharmacopoeia (BP) and the United States Pharmacopoeia (USP) recommend a nonaqueous acid-base titration with potentiometric detection of the end-point for the evaluation of the raw material of metoclopramide; for its dosage forms, the BP describes spectrophotometric methods while the USP recommends highperformance liquid chromatography (HPLC) methods [2]. The drug has been quantified using different methods such as chemiluminescence [3], potentiometry [4], stripping voltammetry [5], liquid chromatography (LC) [6], gas chromatography-mass spectrometry (GC-MS) [7], gas chromatography-electron capture detection [8], high-performance capillary electrophoresis (HPCE) [9] and UV-vis spectrophotometry [10]. High-performance liquid chromatography (HPLC) has become a powerful tool for the analysis of pharmaceutical products and has been applied to the determination of the MCP in biological fluids [11][12][13][14].…”

Determination of Metoclopramide Hydrochloride in Pharmaceutical Formulations Using Three Different Spectrophotometric Methods

“…These methods include voltammetric [4][5][6][7][8][9][10], potentiometric [11][12][13], chromatographic methods [14][15][16][17][18][19]. There is no doubt that some of these methods are sensitive and selective, but they require expensive instruments, accessories, and solvents, not to mention their demand for cleaning procedures.…”