Analysis of Microbial Adhesion of Pathogenic Mushrooms S. Albicans on the Surface of Thermoplastic Material for Orthopedic Constructions

Abstract

Subject: The paper deals with measured biomedical characteristics of samples of a prosthodontic thermoplastic polymer, in regards to certain potentially pathogenic species of oral microflora. Evaluation and comparative analysis of С. albicans adhesion and colonization activity on the prosthodontic material surface were undertaken.
 Materials and methods: The research used the following reference strains: C. albicans, S. aureus, S. epidermidis and Escherichia coli. The biofilms were visualized by means of a plate reader, PowerWave X (USA) after staining with 0.1% aqueous solution of gentian violet and alcohol extraction of the dye. The bacteria were tested for viability by direct inoculation of selective media.
 Results: С. albicans was found to produce a more prominent film on the thermoplastic polymer than other potentially pathogenic microorganisms.
 Conclusions: Satisfactory biomedical characteristics of the thermoplastic material allow it to be used for prosthodontic purposes.