Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing

Abstract

Aged refuse is widely considered to have certain soil fertility. 16S rRNA amplicon sequencing is used to investigate the microbial community of aged refuse. The aged refuse is found to contain higher soil fertility elements (total nitrogen, total phosphorus, total potassium, etc.) and higher concentrations of heavy metals (Pb, Cd, Zn, and Hg). Taxonomy based on operational taxonomic units (OTUs) shows that Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes are the main bacterial phyla in the two soils and there is a palpable difference in the microbial community composition between the two groups of samples. The genera Paramaledivibacter, Limnochorda, Marinobacter, Pseudaminobacter, Kocuria, Bdellovibrio, Halomonas, Gillisia, and Membranicola are enriched in the aged refuse. Functional predictive analysis shows that both the control soil and aged refuse have a high abundance of “carbohydrate metabolism” and “amino acid metabolism”, and show differences in the abundance of several metabolism pathways, such as “xenobiotics biodegradation and metabolism” and “lipid metabolism”. Aged refuse and undisturbed soil show significant differences in alpha diversity and microbial community composition. Multiple environmental factors (Hg, TN, Cr, Cd, etc.) significantly impact microorganisms’ abundance (Marinobacter, Halomonas, Blastococcus, etc.). Our study provides valuable knowledge for the ecological restoration of closed landfills.

Soil metabolomics and bacterial functional traits revealed the responses of rhizosphere soil bacterial community to long-term continuous cropping of Tibetan barley

“…These genera have been frequently detected in the rhizosphere soils after the continuous cropping of different crops, including maize ( Zhao et al, 2021 ), cucumber ( Li et al, 2021 ), cotton ( Han et al, 2017 ), and peanuts ( Chen et al, 2018 ). Solirubrobacter and Blastococcus exhibit heavy metal tolerance and advantages in stress resistance, indicating the promising potential for alleviating polluted soil ecosystems ( Hou et al, 2021 ; Wang et al, 2021 ). A recent study suggested that Sphingomonas possesses multifaceted functions ranging from the remediation of environmental contamination to producing highly beneficial phytohormones that promote plant growth ( Asaf et al, 2020 ).…”