Analysis of Microbiota in Bellflower Root, Platycodon grandiflorum, Obtained from South Korea

Kim, Daeho; Hong, Sanghyun; Na, Hongjun; Chun, Jae Min; Guevarra, Robin B.; Kim, You‐Tae; Ryu, Sangryeol; Kim, Hyeun Bum; Lee, Ju-Hoon

doi:10.4014/jmb.1712.12031

Cited by 4 publications

(2 citation statements)

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“…Briefly, sequences that did not match the PCR primers were eliminated from demultiplexed sequence reads. We also removed sequences containing ambiguous base calls and sequences with a length less than 100 bp to minimize the effects of random sequencing errors [18,19]. Chimeric sequences were further deleted using the UCHIME algorithm implemented in Mothur.…”

Section: S Rrna Gene Sequence Analysismentioning

confidence: 99%

Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

Park

Guevarra

Kim

et al. 2019

Journal of Microbiology and Biotechnology

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Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.

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Section: S Rrna Gene Sequence Analysismentioning

confidence: 99%

Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

Park

Guevarra

Kim

et al. 2019

Journal of Microbiology and Biotechnology

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“…is a perennial herb in the Campanulaceae family that is widely distributed in northeast Asia 1,2 . Platycodon grandiflorum root (PG) is popularly known as “Jiegeng” in China, “Doraji” in Korea, “Kikyo” in Japan, and “Huridunzhaga” in Mongolia 3 and has been widely used to prepare side dishes and kimchi in northeast China and Korea 4,5 . Several studies have indicated that the starch of PG can be made into a high‐quality pastry, and PG extracts can be used as active components in anti‐ageing cosmetics and whitening body lotion 6 .…”

Section: Introductionmentioning

confidence: 99%

Integrated metabolomic and transcriptomic analysis reveals variation in the metabolites and genes of Platycodon grandiflorus roots from different regions

Chang

Pei

et al. 2022

Phytochemical Analysis

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Introduction Platycodon grandiflorum root (PG), a popular traditional Chinese medicine, contains considerable chemical components with broad pharmacological activities. The complexity and diversity of the chemical components of PG from different origins contribute to its broad biological activities. The quality of southern PG is superior to that of northern PG, but the mechanisms underlying these differences remain unclear. Objectives In order to study variation in the differentially accumulated metabolites (DAMs), differentially expressed genes (DEGs), as well as their interactions and signalling pathways among PG from Anhui and Liaoning. Methods The metabolomes based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) and the transcriptome based on high‐throughput sequencing technology were combined to comprehensively analyse PGn and PGb. Results A total of 6515 DEGs and 83 DAMs from the comparison of PG from Anhui and Liaoning were detected. Integrated analysis of metabolomic and transcriptomic data revealed that 215 DEGs and 57 DAMs were significantly enriched in 48 pathways according to KEGG pathway enrichment analysis, and 15 DEGs and 10 DAMs significantly enriched in the main pathway sesquiterpenoid and triterpenoid and phenylpropanoid biosynthesis might play a key role in complex response or regulatory processes. Conclusion Differences in PG from southern and northern China might thus stem from differences in environmental factors, such as precipitation, light duration, and humidity. The results of our study provide new insight into geographic variation in gene expression and metabolite accumulation and will enhance the utilisation of PG resources.

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Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

Paul

Nayak

Thatoi

2020

Journal of Genetic Engineering and Biotechnology

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Background Xylanase has long been recognized as a widely used industrially important enzyme. There are some bacterial species already reported to produce xylanase which have potent xylanolytic activity towards the use of this enzyme in the production of bioethanol from lignocellulosic biomass. In this view, an efficient xylanolytic bacterial strain was isolated and screened from the soil sample of Simlipal Biosphere Reserve. Enzymatic assay for the xylanase activity was evidenced from the most potent bacterial strain, and the culture condition was optimized for obtaining the maximum enzyme activity. The most potent xylanolytic strain was also identified using biochemical and molecular methods. Results Nineteen xylanolytic bacteria (SXB1-SXB19) were isolated from Simlipal forest soil samples following dilution plate technique using corn cob xylan-enriched nutrient agar medium and screened for their xylanase-producing ability. Among these isolates, SXB19 showed maximum xylanolytic potential with a halozone size of 2.5 cm as evident in the formation of prominent yellow patches surrounding its growth in xylan-enriched nutrient agar plate. In unoptimized condition, SXB19 showed the highest enzymatic activity of 22.5 IU/ml among the 19 bacterial strains. In order to optimize the culture conditions for maximizing the xylanase production, Box-Behnken design of response surface methodology (RSM) was used. Four variables such as incubation time, pH, substrate (corn cob xylan) concentration, and temperature were considered for the RSM optimization study. From the results, it is evident that in an optimized condition of incubation time 36 h, pH 6.0, xylan concentration 0.5%, and temperature 42.5 °C, the enzyme activity reached a maximum of 152 IU/ml with nearly 6.75 times increase from the unoptimised condition. Besides, xylanase production from SXB19 was considerable in the presence of xylan followed by starch, nitrogen source such as urea followed by yeast extract, and mineral ion sources such as KCl followed by MgSO4 and ZnSO4. From different biochemical tests, 16S rRNA gene sequencing, and phylogenetic analysis, the bacterial strain SXB19 was identified as Pseudomonas mohnii. Conclusion The isolation of Pseudomonas mohnii, a potent xylanolytic bacterium from Simlipal, is a new report which opens up an opportunity for industrial production of xylanase for bioethanol production and other applications. Graphical abstract

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Analysis of Microbiota in Bellflower Root, Platycodon grandiflorum, Obtained from South Korea

Cited by 4 publications

References 0 publications

Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

Integrated metabolomic and transcriptomic analysis reveals variation in the metabolites and genes of Platycodon grandiflorus roots from different regions

Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

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